Opioid receptors : methods and protocols / edited by Santi M. Spampinato.

Contributor(s): Spampinato, Santi M [editor.]
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 1230.Publisher: New York, NY : Humana Press, [2015]Copyright date: ©2015Description: 1 online resource (xv, 326 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493917082; 1493917080; 1493917072; 9781493917075Subject(s): Opioids -- Receptors -- Laboratory manuals | Receptors, Opioid | Neurosciences | Pharmacology | Medical -- Pharmacology | Science -- Life Sciences -- Zoology -- General | Opioids -- Receptors | farmacologie | pharmacology | toxicologie | toxicology | biomedische wetenschappen | biomedicine | neurobiologie | neurobiology | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Opioid receptors.DDC classification: 615.7/822 LOC classification: RB127Online resources: Click here to access online
Contents:
Overview of genetic analysis of human opioid receptors / Santi M. Spampinato -- Computational structural biology of opioid receptors / Davide Provasi -- Analysis of epigenetic mechanisms regulating opioid receptor gene transcription / Cheol Kyu Hwang [and three others] -- Renilla luciferase reporter assay to study 3'UTR-Driven posttranscriptional regulations of OPRM1 / Gabriele Vincelli and Andrea Bedini -- High-throughput gene expression profiling of opioid-induced alterations in discrete brain areas / Michal Korostynski [and three others] -- Real-time imaging of mu opioid receptors by total internal reflection fluorescence microscopy / Cristina Roman-Vendrell and Guillermo Ariel Yudowski -- In vivo techniques to investigate the internalization profile of opioid receptors / Amynah A. Pradhan [and three others] -- Monitoring opioid receptor dimerization in living cells by bioluminescence resonance energy transfer (BRET) / Monica Baiula.
Analysis of potassium and calcium imaging to assay the function of opioid receptors / Viola Spahn, Dinah Nockemann, and Halina Machelska -- Electrophysiological patch clamp assay to monitor the action of opioid receptors / Viola Spahn, Dinah Nockemann, and Halina Machelska -- Skin-nerve preparation to assay the function of opioid receptors in peripheral endings of sensory neurons / Rabih Moshourab, Yvonne Schmidt, and Halina Machelska -- Mechanical nociception measurement in mice and rats with automated Von Frey equipment / Gabriele Campana and Roberto Rimondini -- Detecting zinc release induced by mu-opioid receptor agonists in brain slices / María Rodríguez-Muñoz [and three others] -- Opioid receptors : methods for detection and their modes of actions in the eye / Shahid Husain -- Evaluation of murine macrophage cytokine production after in vivo morphine treatment / Silvia Franchi [and four others].
Measurement of macrophage toll-like receptor 4 expression after morphine treatment / Mara Castelli [and three others] -- The role of opioid receptors in migration and wound recovery in vitro in cultured human keratinocytes and fibroblasts / Mei Bigliardi-Qi and Paul L. Bigliardi -- Role of opioid receptors in the reinstatement of opioid- seeking behavior : an overview / Liana Fattore [and three others] -- Analysis of opioid-seeking reinstatement in the rat / Liana Fattore [and three others] -- Induction of a high alcohol consumption in rats and mice : role of opioid receptors / Roberto Rimondini and Gabriele Campana -- Evaluation of social and nonsocial behaviors mediated by opioids in mouse pups / Francesca R. D'Amato.
Summary: Opioid Receptors: Methods and Protocols serves as a comprehensive guide to both key new techniques and established methods for the investigation of genetics, structural biology, transcription, and post-transcriptional events of opioid receptors. Other methods cover the cellular detection and trafficking of opioid receptors in vitro and in vivo. Procedures aimed to investigate signaling pathways modulated by opioid receptors and model systems to study opioid receptor-mediated functions are also included. Finally, methods to assay behavioral effects mediated by opioid receptors are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Wide-ranging and authoritative, Opioid Receptors: Methods and Protocols will help both experienced and new entrants in this field to carry out their experiments successfully and with new inspiration.
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Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed October 28, 2014).

Opioid Receptors: Methods and Protocols serves as a comprehensive guide to both key new techniques and established methods for the investigation of genetics, structural biology, transcription, and post-transcriptional events of opioid receptors. Other methods cover the cellular detection and trafficking of opioid receptors in vitro and in vivo. Procedures aimed to investigate signaling pathways modulated by opioid receptors and model systems to study opioid receptor-mediated functions are also included. Finally, methods to assay behavioral effects mediated by opioid receptors are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Wide-ranging and authoritative, Opioid Receptors: Methods and Protocols will help both experienced and new entrants in this field to carry out their experiments successfully and with new inspiration.

Overview of genetic analysis of human opioid receptors / Santi M. Spampinato -- Computational structural biology of opioid receptors / Davide Provasi -- Analysis of epigenetic mechanisms regulating opioid receptor gene transcription / Cheol Kyu Hwang [and three others] -- Renilla luciferase reporter assay to study 3'UTR-Driven posttranscriptional regulations of OPRM1 / Gabriele Vincelli and Andrea Bedini -- High-throughput gene expression profiling of opioid-induced alterations in discrete brain areas / Michal Korostynski [and three others] -- Real-time imaging of mu opioid receptors by total internal reflection fluorescence microscopy / Cristina Roman-Vendrell and Guillermo Ariel Yudowski -- In vivo techniques to investigate the internalization profile of opioid receptors / Amynah A. Pradhan [and three others] -- Monitoring opioid receptor dimerization in living cells by bioluminescence resonance energy transfer (BRET) / Monica Baiula.

Analysis of potassium and calcium imaging to assay the function of opioid receptors / Viola Spahn, Dinah Nockemann, and Halina Machelska -- Electrophysiological patch clamp assay to monitor the action of opioid receptors / Viola Spahn, Dinah Nockemann, and Halina Machelska -- Skin-nerve preparation to assay the function of opioid receptors in peripheral endings of sensory neurons / Rabih Moshourab, Yvonne Schmidt, and Halina Machelska -- Mechanical nociception measurement in mice and rats with automated Von Frey equipment / Gabriele Campana and Roberto Rimondini -- Detecting zinc release induced by mu-opioid receptor agonists in brain slices / María Rodríguez-Muñoz [and three others] -- Opioid receptors : methods for detection and their modes of actions in the eye / Shahid Husain -- Evaluation of murine macrophage cytokine production after in vivo morphine treatment / Silvia Franchi [and four others].

Measurement of macrophage toll-like receptor 4 expression after morphine treatment / Mara Castelli [and three others] -- The role of opioid receptors in migration and wound recovery in vitro in cultured human keratinocytes and fibroblasts / Mei Bigliardi-Qi and Paul L. Bigliardi -- Role of opioid receptors in the reinstatement of opioid- seeking behavior : an overview / Liana Fattore [and three others] -- Analysis of opioid-seeking reinstatement in the rat / Liana Fattore [and three others] -- Induction of a high alcohol consumption in rats and mice : role of opioid receptors / Roberto Rimondini and Gabriele Campana -- Evaluation of social and nonsocial behaviors mediated by opioids in mouse pups / Francesca R. D'Amato.

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