Insoluble proteins : methods and protocols / edited by Elena Garcia-Fruitos.

Contributor(s): García-Fruitós, Elena [editor.]
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 1258.Publisher: New York : Humana Press : Springer, [2015]Copyright date: ©2015Description: 1 online resource (xvi, 425 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493922055; 149392205XSubject(s): Recombinant proteins -- Laboratory manuals | Proteins -- Structure | Molecular Conformation | Technology | Biological Science Disciplines | Amino Acids, Peptides, and Proteins | Molecular Structure | Chemicals and Drugs | Technology, Industry, and Agriculture | Natural Science Disciplines | Technology, Industry, Agriculture | Disciplines and Occupations | Biochemical Phenomena | Chemical Phenomena | Phenomena and Processes | Proteins | Biotechnology | Protein Conformation | Science -- Life Sciences -- Biochemistry | Proteins | Proteins -- Structure | Recombinant proteinsGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Insoluble proteins.DDC classification: 572.6 LOC classification: TP248.65.P76QD431-431.7Online resources: Click here to access online
Contents:
General introduction : recombinant protein production and purification of insoluble proteins / Neus Ferrer-Miralles [and 4 others] -- Overcoming the solubility problem in E. coli : available approaches for recombinant protein production / Agustín Correa and Pablo Oppezzo -- Optimization of culture parameters and novel strategies to improve protein solubility / Ranjana Arya [and 3 others] -- Cleavable self-aggregating tags (cSAT) for protein expression and purification / Zhanglin Lin [and 4 others] -- Beyond the cytoplasm of Escherichia coli : localizing recombinant proteins where you want them / Jason T. Boock [and 3 others] -- Characterization of amyloid-like properties in bacterial intracellular aggregates / Anna Villar-Pique, Susanna Navarro, and Salvador Ventura -- Co-translational stabilization of insoluble proteins in cell- free expression systems / Lei Kai [and 5 others] -- Functional expression of plant membrane proteins in Lactococcus lactis / Sylvain Boutigny [and 6 others] -- High cell-density expression system : yeast cells in a phalanx efficiently produce a certain range of "Difficult-to-Express" secretory recombinant proteins / Yasuaki Kawarasaki, Takeshi Kurose, and Keisuke Ito -- Insect cells-baculovirus system for the production of difficult to express proteins / Judit Osz-Papai [and 6 others] -- Transient expression in HEK 293 cells : an alternative to E. coli for the production of secreted and intracellular mammalian proteins / Joanne E. Nettleship [and 11 others] -- Recombinant glycoprotein production in human cell lines / Kamilla Swiech [and 4 others] -- Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 / Maria Giuliani [and 5 others] -- Screening methodology for purifying proteins with aggregation problems / Mario Lebendiker, Michal Maes, and Assaf Friedler -- Solubilization and refolding of inclusion body proteins / Anupam Singh, Vaibhav Upadhyay, and Amulya K. Panda -- Bacterial inclusion body purification / Joaquin Seras-Franzoso [and 4 others] -- Characterization of intracellular aggresomes by fluorescent microscopy / Lianwu Fu and Elizabeth Sztul -- Dialysis : a characterization method of aggregation tendency / Mireia Pesarrodona, Ugutz Unzueta, and Esther Vázquez -- Applications of mass spectrometry to the study of protein aggregation / Sílvia Bronsoms and Sebastián A. Trejo -- Insoluble protein assemblies characterized by Fourier transform infrared spectroscopy / Antonino Natalello and Silvia M. Doglia -- Insoluble protein characterization by circular dichroism (CD) spectroscopy and nuclear magnetic resonance (NMR) / Shaveta Goyal [and 3 others] -- Methods for characterization of protein aggregates / Witold Tatkiewicz [and 6 others] -- Predicting the solubility of recombinant proteins in Escherichia coli / Roger G. Harrison and Miguel J. Bagajewicz -- Insoluble protein applications : the use of bacterial inclusion bodies as biocatalysts / Eva Hrabárová, Lucia Achbergerová, and Jozef Nahálka.
Summary: With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.
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Includes bibliographical references and index.

General introduction : recombinant protein production and purification of insoluble proteins / Neus Ferrer-Miralles [and 4 others] -- Overcoming the solubility problem in E. coli : available approaches for recombinant protein production / Agustín Correa and Pablo Oppezzo -- Optimization of culture parameters and novel strategies to improve protein solubility / Ranjana Arya [and 3 others] -- Cleavable self-aggregating tags (cSAT) for protein expression and purification / Zhanglin Lin [and 4 others] -- Beyond the cytoplasm of Escherichia coli : localizing recombinant proteins where you want them / Jason T. Boock [and 3 others] -- Characterization of amyloid-like properties in bacterial intracellular aggregates / Anna Villar-Pique, Susanna Navarro, and Salvador Ventura -- Co-translational stabilization of insoluble proteins in cell- free expression systems / Lei Kai [and 5 others] -- Functional expression of plant membrane proteins in Lactococcus lactis / Sylvain Boutigny [and 6 others] -- High cell-density expression system : yeast cells in a phalanx efficiently produce a certain range of "Difficult-to-Express" secretory recombinant proteins / Yasuaki Kawarasaki, Takeshi Kurose, and Keisuke Ito -- Insect cells-baculovirus system for the production of difficult to express proteins / Judit Osz-Papai [and 6 others] -- Transient expression in HEK 293 cells : an alternative to E. coli for the production of secreted and intracellular mammalian proteins / Joanne E. Nettleship [and 11 others] -- Recombinant glycoprotein production in human cell lines / Kamilla Swiech [and 4 others] -- Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 / Maria Giuliani [and 5 others] -- Screening methodology for purifying proteins with aggregation problems / Mario Lebendiker, Michal Maes, and Assaf Friedler -- Solubilization and refolding of inclusion body proteins / Anupam Singh, Vaibhav Upadhyay, and Amulya K. Panda -- Bacterial inclusion body purification / Joaquin Seras-Franzoso [and 4 others] -- Characterization of intracellular aggresomes by fluorescent microscopy / Lianwu Fu and Elizabeth Sztul -- Dialysis : a characterization method of aggregation tendency / Mireia Pesarrodona, Ugutz Unzueta, and Esther Vázquez -- Applications of mass spectrometry to the study of protein aggregation / Sílvia Bronsoms and Sebastián A. Trejo -- Insoluble protein assemblies characterized by Fourier transform infrared spectroscopy / Antonino Natalello and Silvia M. Doglia -- Insoluble protein characterization by circular dichroism (CD) spectroscopy and nuclear magnetic resonance (NMR) / Shaveta Goyal [and 3 others] -- Methods for characterization of protein aggregates / Witold Tatkiewicz [and 6 others] -- Predicting the solubility of recombinant proteins in Escherichia coli / Roger G. Harrison and Miguel J. Bagajewicz -- Insoluble protein applications : the use of bacterial inclusion bodies as biocatalysts / Eva Hrabárová, Lucia Achbergerová, and Jozef Nahálka.

With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.

English.

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