Mycobacteria protocols / edited by Tanya Parish and David M. Roberts.

Contributor(s): Parish, Tanya | Roberts, David M
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 1285.Publisher: New York, NY : Humana Press : Springer, ©2015Edition: Third editionDescription: 1 online resource (xii, 416 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493924509; 1493924508; 1493924494; 9781493924493Subject(s): Mycobacteria -- Laboratory manuals | Mycobacterium | Genetic Techniques | Molecular Biology -- methods | Cytological Techniques | Mycobacteria | bacteria | microbiologie | microbiology | Microbiology (General) | Microbiologie (algemeen)Genre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Mycobacteria protocols.DDC classification: 579.3/74 LOC classification: QR82.M8Online resources: Click here to access online
Contents:
Whole-genome sequencing for comparative genomics and de novo genome assembly / Andrej Benjak, Claudia Sala, and Ruben C. Hartkoorn -- Whole-transcriptome sequencing for high-resolution transcriptomic analysis in mycobacterium tuberculosis / Andrej Benjak, Claudia Sala, and Ruben C. Hartkoorn -- RNA sequencing for transcript 5'-end mapping in mycobacteria / Scarlet S. Shell [and 3 otherst] -- Fractionation and analysis of mycobacterial proteins / Megan C. Lucas [and 6 others] -- Lipid and lipoarabinomannan isolation and characterization / Marie-Antoinette Lanéelle, Jérôme Nigou, and Mamadou Daffé -- Metabolomics of mycobacterium tuberculosis / Madhumitha Nandakumar [and 3 others] -- Electroporation of mycobacteria / Renan Goude, David M. Roberts, and Tanya Parish -- Targeted gene knockout and essentiality testing by homologous recombination / Krishnamoorthy Gopinath, Digby F. Warner, and Valerie Mizrahi -- Construction of conditional knockdown mutants in mycobacteria / Dirk Schnappinger, Kathryn M. O'Brien, and Sabine Ehrt -- Mycobacterial recombineering / Kenan C. Murphy, Kadamba Papavinasasundaram, and Christopher M. Sassetti -- In vitro models that utilize hypoxia to induce non-replicating persistence in mycobacteria / Charles D. Sohaskeyand Martin I. Voskuil -- Genetic dissection of mycobacterial biofilms / Anil K. Ojha, William R. Jacobs Jr., and Graham F. Hatfull -- Measuring efflux and permeability in mycobacteria / Liliana Rodrigues, Miguel Viveiros, and José A. Aínsa -- Single-cell analysis of mycobacteria using microfluidics and time-lapse microscopy / Neeraj Dharand Giulia Manina -- Antimicrobial susceptibility testing for Mycobacterium sp. / Delia Blanco-Ruano [and 6 others] -- Determination of compound kill kinetics against mycobacterium tuberculosis / Julie Early and Torey Alling -- Microplate alamar blue assay (MABA) and low oxygen recovery assay (LORA) for mycobacterium tuberculosis Sanghyun Cho, Hyung Sup Lee, and Scott Franzblau -- Multi-stress model for high throughput screening against non-replicating mycobacterium tuberculosis / Ben Gold, Thulasi Warrier, and Carl Nathan -- Isolation and characterization of compound-resistant isolates of mycobacterium tuberculosis / Theresa O'Malley and Eduard Melief -- Macrophage infection models for mycobacterium tuberculosis / Benjamin K. Johnson and Robert B. Abramovitch -- Infection of human neutrophils to study virulence properties of mycobacterium tuberculosis / Tobias Dallenga, Björn Corleis, and Ulrich E. Schaible -- Isolation of bead phagosomes to study virulence function of M. tuberculosis cell wall lipids / Anna C. Geffken, Emmanuel C. Patin, and Ulrich E. Schaible -- Live imaging of mycobacterium marinum infection in dictyostelium discoideum / Caroline Barisch, Ana T. López-Jiménez, and Thierry Soldati -- Testing chemical and genetic modulators in mycobacterium tuberculosis infected cells using phenotypic assays / Vincent Delorme [and 3 others].
Summary: Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory. {OCLCbr#A0}Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites.{OCLCbr#A0} Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. {OCLCbr#A0}Authoritative and up-to-date, Mycobacteria Protocols, Third Edition will be a resource both to those working in the field, and to newcomers.
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Includes bibliographical references and index.

Whole-genome sequencing for comparative genomics and de novo genome assembly / Andrej Benjak, Claudia Sala, and Ruben C. Hartkoorn -- Whole-transcriptome sequencing for high-resolution transcriptomic analysis in mycobacterium tuberculosis / Andrej Benjak, Claudia Sala, and Ruben C. Hartkoorn -- RNA sequencing for transcript 5'-end mapping in mycobacteria / Scarlet S. Shell [and 3 otherst] -- Fractionation and analysis of mycobacterial proteins / Megan C. Lucas [and 6 others] -- Lipid and lipoarabinomannan isolation and characterization / Marie-Antoinette Lanéelle, Jérôme Nigou, and Mamadou Daffé -- Metabolomics of mycobacterium tuberculosis / Madhumitha Nandakumar [and 3 others] -- Electroporation of mycobacteria / Renan Goude, David M. Roberts, and Tanya Parish -- Targeted gene knockout and essentiality testing by homologous recombination / Krishnamoorthy Gopinath, Digby F. Warner, and Valerie Mizrahi -- Construction of conditional knockdown mutants in mycobacteria / Dirk Schnappinger, Kathryn M. O'Brien, and Sabine Ehrt -- Mycobacterial recombineering / Kenan C. Murphy, Kadamba Papavinasasundaram, and Christopher M. Sassetti -- In vitro models that utilize hypoxia to induce non-replicating persistence in mycobacteria / Charles D. Sohaskeyand Martin I. Voskuil -- Genetic dissection of mycobacterial biofilms / Anil K. Ojha, William R. Jacobs Jr., and Graham F. Hatfull -- Measuring efflux and permeability in mycobacteria / Liliana Rodrigues, Miguel Viveiros, and José A. Aínsa -- Single-cell analysis of mycobacteria using microfluidics and time-lapse microscopy / Neeraj Dharand Giulia Manina -- Antimicrobial susceptibility testing for Mycobacterium sp. / Delia Blanco-Ruano [and 6 others] -- Determination of compound kill kinetics against mycobacterium tuberculosis / Julie Early and Torey Alling -- Microplate alamar blue assay (MABA) and low oxygen recovery assay (LORA) for mycobacterium tuberculosis Sanghyun Cho, Hyung Sup Lee, and Scott Franzblau -- Multi-stress model for high throughput screening against non-replicating mycobacterium tuberculosis / Ben Gold, Thulasi Warrier, and Carl Nathan -- Isolation and characterization of compound-resistant isolates of mycobacterium tuberculosis / Theresa O'Malley and Eduard Melief -- Macrophage infection models for mycobacterium tuberculosis / Benjamin K. Johnson and Robert B. Abramovitch -- Infection of human neutrophils to study virulence properties of mycobacterium tuberculosis / Tobias Dallenga, Björn Corleis, and Ulrich E. Schaible -- Isolation of bead phagosomes to study virulence function of M. tuberculosis cell wall lipids / Anna C. Geffken, Emmanuel C. Patin, and Ulrich E. Schaible -- Live imaging of mycobacterium marinum infection in dictyostelium discoideum / Caroline Barisch, Ana T. López-Jiménez, and Thierry Soldati -- Testing chemical and genetic modulators in mycobacterium tuberculosis infected cells using phenotypic assays / Vincent Delorme [and 3 others].

Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory. {OCLCbr#A0}Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites.{OCLCbr#A0} Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. {OCLCbr#A0}Authoritative and up-to-date, Mycobacteria Protocols, Third Edition will be a resource both to those working in the field, and to newcomers.

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