Pyrosequencing : methods and protocols / edited by Ulrich Lehmann, Jörg Tost.
Contributor(s): Lehmann, Ulrich [editor.] | Tost, Jörg [editor.]Material type: TextSeries: Methods in molecular biology (Clifton, N.J.): v. 1315.Publisher: New York, NY : Humana Press, 2015Edition: Second editionDescription: 1 online resource (xiv, 414 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493927159; 1493927159; 1493927140; 9781493927142Subject(s): Genetics -- Technique -- Laboratory manuals | Nucleotide sequence -- Laboratory manuals | Sequence Analysis, DNA -- methods | High-Throughput Nucleotide Sequencing -- methods | Genetics -- Technique | Nucleotide sequence | biomedische wetenschappen | biomedicine | moleculaire genetica | molecular genetics | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Pyrosequencing.DDC classification: 572.8 LOC classification: QH441Online resources: Click here to access online
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Online resource; title from PDF title page (SpringerLink, viewed July 2, 2015).
Annotation The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns associated with specific histone modifications. The volume further details tools and protocols for the detection of viruses and bacteria, and genetic and epigenetic analyses for forensics using Pyrosequencing. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.Comprehensive and adaptable, Pyrosequencing: Methods and Protocols, Second Edition will greatly aid doctorial students, postdoctoral investigators and research scientists studying different aspects of genetics and cellular and molecular biology.
Part I. Introduction. The History of Pyrosequencing® / Nyrén, Pål -- PyroMark® Instruments, Chemistry and Software / Kreutz, Martin (and others) -- Software-Based Pyrogram® Evaluation / Chen, Guoli (and others) -- Quantitative Validation and Quality Control of Pyrosequencing® Assays / Lehmann, Ulrich -- Part II. Analysis of Genetic Variation. Extended KRAS and NRAS Mutation Profiling by Pyrosequencing® Assays / Jung, Andreas -- Universal BRAF State Detection by the Pyrosequencing®-Based U-BRAFv600 Assay / Skorokhod, Alexander -- Pyrosequencing®-Based Identification of Low-Frequency Mutations Enriched through Enhance ice-COLD-PCR / How-Kit, Alexandre and Jörg Tost -- Analysis of Mutational Hotspots in Routinely Processed Bone Marrow Trephines by Pyrosequencing® / Bartels, Stephan and Ulrich Lehmann -- Analysis of Copy Number Variation by Pyrosequencing® Using Paralogous Sequences / Kringen, Marianne Kristiansen -- Prenatal Diagnosis of Chromosomal Aneuploidies by Quantitative Pyrosequencing® / Ye, Hui (and others) -- HLA-B and HLA-C Supratyping by Pyrosequencing® / Vanni, Irene (and others) -- Allele Quantification Pyrosequencing® at Designated SNP Sites to Detect Allelic Expression Imbalance and Loss-of-Heterozygosity / Kwok, Chau-To and Megan P. Hitchins -- Part III. Analysis of DNA Methylation Patterns. Quantitative DNA Methylation Analysis by Pyrosequencing® / Roessler, Jessica and Ulrich Lehmann -- Quantitative Methylation Analysis of the PCDHB Gene Cluster / Banelli, Barbara and Massimo Romani -- Assessment of Changes in Global DNA Methylation Levels by Pyrosequencing® of Repetitive Elements / Tabish, Ali M. (and others) -- Global Analysis of DNA 5-Methylcytosine Using the Luminometric Methylation Assay, LUMA / Luttropp, Karin (and others) -- Limiting Dilution Bisulfite Pyrosequencing®: a Method for Methylation Analysis of Individual DNA Molecules in a Single or a Few Cells / Hajj, Nady El (and others) -- Detection of Loss of Imprinting by Pyrosequencing® / Tabano, Silvia (and others) -- Analysis of DNA Methylation Patterns in Single Blastocysts by Pyrosequencing® / Huntriss, John (and others) -- Allele-Specific DNA Methylation Detection by Pyrosequencing® / Kristensen, Lasse Sommer (and others) -- SNP-Based Quantification of Allele-Specific DNA Methylation Patterns by Pyrosequencing® / Busato, Florence and Jörg Tost -- DNA Methylation Analysis of ChIP Products at Single Nucleotide Resolution by Pyrosequencing® / Moison, Céline (and others) -- Part IV. Bacterial Typing and Identification. Multiplex Pyrosequencing®: Simultaneous Genotyping Based on SNPs from Distant Genomic Regions / Dabrowski, Piotr Wojciech (and others) -- Detection of Drug-Resistant Mycobacterium tuberculosis / Engström, Anna and Pontus Jureen -- Application of Pyrosequencing® in Food Biodefense / Amoako, Kingsley Kwaku -- Forensic Analysis of Mitochondrial and Autosomal Markers Using Pyrosequencing® / Buś, Magdalena M. (and others) -- Tissue-Specific DNA Methylation Patterns in Forensic Samples Detected by Pyrosequencing® / Antunes, Joana (and others).
Includes bibliographical references and index.