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Ionotropic glutamate receptor technologies / edited by Gabriela K. Popescu.

Contributor(s): Popescu, Gabriela K [editor.]Material type: TextTextSeries: Neuromethods ; 106.Publisher: New York, NY : Humana Press, [2015]Copyright date: ©2015Description: 1 online resource (xii, 304 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493928125; 1493928120; 1493928112; 9781493928118Subject(s): Glutamic acid -- Receptors -- Laboratory manuals | Ion channels -- Laboratory manuals | Neurotransmitters -- Laboratory manuals | Receptors, Glutamate -- physiology | Ion Channels | Receptors, Neurotransmitter | Medical -- Physiology | Physiology | Medical -- Neuroscience | Neurosciences | Glutamic acid -- Receptors | Ion channels | NeurotransmittersGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Printed edition:: No titleDDC classification: 573.8/5 LOC classification: QP364.7Online resources: Click here to access online
Contents:
Assaying AMPA Receptor Oligomerization -- A Step-by-Step Guide to Single Subunit Counting Of Membrane-Bound Proteins in Mammalian Cells -- Counting NMDA Receptors at the Cell Surface -- Electron Microscopy Analysis of AMPA receptors in Dendritic Spines -- Electrophysiological Tagging of Ionotropic Receptors -- Functional Detection of Novel Triheteromeric NMDA Receptors -- Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses -- NMR Approaches to Functional Dynamics Of Genetically Separated iGluR Domains -- Computing Conformational Free Energies of iGluR Ligand-Binding Domains -- LRET Methods for Investigating Conformational Changes in Functional Ionotropic Glutamate Receptors -- Assaying the Energetics of NMDA Receptor Pore Opening -- Constructing a Rapid Solution Exchange System -- Assessing Effects of Ligand-Binding Mutations To AMPA And Kainate Receptor Kinetics -- Analysis of Whole-Cell NMDA Receptor Responses -- Expression, Purification and Crystallization of Full Length Ionotropic Glutamate Receptors -- Timing AMPA Receptor Activation with Laser-pulse Photolysis -- Current Recording and Kinetic Analyses for Single AMPA Receptors -- Extracting Rate Constants for NMDA Receptor Gating from One-channel Current Recordings.
Summary: This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology. Chapters in the book cover methods to help illuminate the assembly, trafficking, molecular composition and subcellular location of iGluRs; approaches used to understand the atomic organization of iGluRs; and techniques to monitor receptor activity in real time.
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Includes index.

Online resource; title from PDF title page (SpringerLink, viewed October 7, 2015).

This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology. Chapters in the book cover methods to help illuminate the assembly, trafficking, molecular composition and subcellular location of iGluRs; approaches used to understand the atomic organization of iGluRs; and techniques to monitor receptor activity in real time.

Assaying AMPA Receptor Oligomerization -- A Step-by-Step Guide to Single Subunit Counting Of Membrane-Bound Proteins in Mammalian Cells -- Counting NMDA Receptors at the Cell Surface -- Electron Microscopy Analysis of AMPA receptors in Dendritic Spines -- Electrophysiological Tagging of Ionotropic Receptors -- Functional Detection of Novel Triheteromeric NMDA Receptors -- Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses -- NMR Approaches to Functional Dynamics Of Genetically Separated iGluR Domains -- Computing Conformational Free Energies of iGluR Ligand-Binding Domains -- LRET Methods for Investigating Conformational Changes in Functional Ionotropic Glutamate Receptors -- Assaying the Energetics of NMDA Receptor Pore Opening -- Constructing a Rapid Solution Exchange System -- Assessing Effects of Ligand-Binding Mutations To AMPA And Kainate Receptor Kinetics -- Analysis of Whole-Cell NMDA Receptor Responses -- Expression, Purification and Crystallization of Full Length Ionotropic Glutamate Receptors -- Timing AMPA Receptor Activation with Laser-pulse Photolysis -- Current Recording and Kinetic Analyses for Single AMPA Receptors -- Extracting Rate Constants for NMDA Receptor Gating from One-channel Current Recordings.

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