Mammary gland development : methods and protocols / edited by Finian Martin, Torsten Stein, Jillian Howlin.

Contributor(s): Martin, Finian [editor.] | Stein, Torsten [editor.] | Howlin, Jillian [editor.]
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 1501.Publisher: New York, NY : Humana Press, [2017]Copyright date: ©2017Description: 1 online resource (xi, 358 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493964758; 1493964755; 1493964739; 9781493964734Subject(s): Mammary glands -- Growth -- Laboratory manuals | Mammary Glands, Animal -- growth & development | Mammary Glands, Human -- growth & development | Breast Neoplasms -- genetics | Stem Cells | Genetic Techniques | Mammary glands -- GrowthGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Mammary gland development.DDC classification: 573.6/79 LOC classification: QP188.M3Online resources: Click here to access online
Contents:
Overview of mammary gland development: A comparison of mouse and human -- Prenatal mammary gland development in the mouse: Research models and techniques for its study from past to present -- Pubertal mammary gland development: Elucidation of in vivo morphogenesis using murine models -- Analysis of mammary gland phenotypes by transplantation of the genetically marked mammary epithelium -- Pubertal ductal morphogenesis: Isolation and transcriptome analysis of the terminal end bud -- RNA profiling of non-cultured fibroblasts isolated from pubertal mouse mammary gland sections -- Analysis of the involuting mouse mammary gland: An in vivo model for cell death -- Contractility assay for established myoepithelial cell lines -- Using 3d culture of primary mammary epithelial cells to define molecular entities required for acinus formation: Analyzing MAP kinase phosphatases -- A 3d fibroblast-epithelium co-culture model for understanding microenvironmental role in branching morphogenesis of the mammary gland -- Next generation rna sequencing analysis reveals expression of a transient EMT profile during early organization of MCF10a cells in 3d -- A 3d culture model to study how fluid pressure and flow affect the behavior of aggregates of epithelial cells -- Purification of distinct subsets of epithelial cells from normal human breast tissue -- Techniques for the reprogramming of exogenous stem/progenitor cell populations towards a mammary epithelial cell fate -- Lineage tracing of mammary stem and progenitor cells -- Assessment of significance of novel proteins in breast cancer using tissue microarray technology -- Patient-derived xenografts of breast cancer.
Summary: This volume provides key molecular biological protocols and experimental strategies currently employed to study the various stages of mammary gland development. Chapters are divided into four parts covering genetically modified mouse models that exhibit prenatal and pubertal mammary gland phenotypes, 2D and 3D-model culture systems, methodologies for mammary stem cell isolation, and translational applications that provide a bridge between experimental studies of mammary gland development and the study of human breast cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammary Gland Development: Methods and Protocols will be useful for researchers whose primary interest is in mammary gland development; developmental biologists, epithelial cell biologists, and those with an interest in molecular mechanisms underlying breast cancer.
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Overview of mammary gland development: A comparison of mouse and human -- Prenatal mammary gland development in the mouse: Research models and techniques for its study from past to present -- Pubertal mammary gland development: Elucidation of in vivo morphogenesis using murine models -- Analysis of mammary gland phenotypes by transplantation of the genetically marked mammary epithelium -- Pubertal ductal morphogenesis: Isolation and transcriptome analysis of the terminal end bud -- RNA profiling of non-cultured fibroblasts isolated from pubertal mouse mammary gland sections -- Analysis of the involuting mouse mammary gland: An in vivo model for cell death -- Contractility assay for established myoepithelial cell lines -- Using 3d culture of primary mammary epithelial cells to define molecular entities required for acinus formation: Analyzing MAP kinase phosphatases -- A 3d fibroblast-epithelium co-culture model for understanding microenvironmental role in branching morphogenesis of the mammary gland -- Next generation rna sequencing analysis reveals expression of a transient EMT profile during early organization of MCF10a cells in 3d -- A 3d culture model to study how fluid pressure and flow affect the behavior of aggregates of epithelial cells -- Purification of distinct subsets of epithelial cells from normal human breast tissue -- Techniques for the reprogramming of exogenous stem/progenitor cell populations towards a mammary epithelial cell fate -- Lineage tracing of mammary stem and progenitor cells -- Assessment of significance of novel proteins in breast cancer using tissue microarray technology -- Patient-derived xenografts of breast cancer.

This volume provides key molecular biological protocols and experimental strategies currently employed to study the various stages of mammary gland development. Chapters are divided into four parts covering genetically modified mouse models that exhibit prenatal and pubertal mammary gland phenotypes, 2D and 3D-model culture systems, methodologies for mammary stem cell isolation, and translational applications that provide a bridge between experimental studies of mammary gland development and the study of human breast cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammary Gland Development: Methods and Protocols will be useful for researchers whose primary interest is in mammary gland development; developmental biologists, epithelial cell biologists, and those with an interest in molecular mechanisms underlying breast cancer.

Includes index.

Online resource; title from PDF title page (SpringerLink, viewed November 2, 2016).

Includes bibliographical references and index.

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