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Mitochondria : practical protocols / edited by Dejana Mokranjac, Fabiana Perocchi.

Contributor(s): Mokranjac, Dejana [editor.] | Perocchi, Fabiana [editor.]Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1567. | Springer protocols (Series)Publisher: New York : Humana Press imprint is published by Springer Nature, [2017]Copyright date: ©2017Edition: Second editionDescription: 1 online resource (xi, 409 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493968244; 1493968246Uniform titles: Mitochondria (New York, N.Y.) Subject(s): Mitochondria -- Laboratory manuals | Mitochondria -- physiology | Mitochondria -- genetics | Mitochondrial Proteins | Models, Biological | Cellular biology (cytology) | Science -- Life Sciences -- Biology -- Molecular Biology | MitochondriaGenre/Form: Electronic books. | Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Mitochondria.DDC classification: 571.6/57 LOC classification: QH603.M5NLM classification: QU 25Online resources: Click here to access online
Contents:
Guide to computational methods for predicting mitochondrial localization / Su Sun and Bianca H. Habermann -- Isolation of functional mitochondria from cultured cells and mouse tissues / Jennifer Wettmarshausen and Fabiana Perocchi -- Isolation of mitochondria from Saccharomyces cerevisiae / Toshiaki Izawa and Ann-Katrin Unger -- Isolation of contact sites between inner and outer mitochondrial membranes / Max Harner -- Isolation of mitochondria-associated membranes (MAM) from mouse brain tissue / Bernadette Schreiner and Maria Ankarcrona -- Label-free quantitative analysis of mitochondrial proteomes using the multienzyme digestion-filter aided sample preparation (MED-FASP) and "Total Protein Approach" / Jacek R. Wiśniewski -- Quantitative analysis of glycerophospholipids in mitochondria by mass spectrometry / Takashi Tatsuta -- Detection of cysteine redox states in mitochondrial proteins in intact mammalian cells / Markus Habich and Jan Riemer -- Chemical crosslinking in intact mitochondria / Rupa Banerjee, Umut Günsel, and Dejana Mokranjac -- Reconstitution of mitochondrial membrane proteins into nanodiscs by cell-free expression / Ketan Malhotra and Nathan N. Alder -- Detection of dual targeting and dual function of mitochondrial proteins in yeast / Reut Ben-Menachem and Ophry Pines -- Localizing mRNAs encoding mitochondrial proteins in yeast by fluorescence microscopy and subcellular fractionation / Dmitry Zabezhinsky, Hannah Sperber, and Jeffrey E. Gerst -- Assessing mitochondrial bioenergetics in isolated mitochondria from various mouse tissues using seahorse XF96 analyzer / Arcangela Iuso, Birgit Repp, Caroline Biagosch, Caterina Terrile, and Holger Prokisch -- Application of FRET-based biosensor "ATeam" for visualization of ATP levels in the mitochondrial matrix of living mammalian cells / Tomoki Yoshida, Soaad Alfaqaan, Norio Sasaoka, and Hiromi Imamura -- Microplate-based bioluminescence assay of mitochondrial calcium uptake / María Teresa Alonso, Paloma Navas-Navarro, and Javier García-Sancho -- New imaging tools to analyze mitochondrial morphology in Caenorhabditis elegans / Saroj G. Regmi and Stéphane G. Rolland -- Single molecule tracking and localization of mitochondrial protein complexes in live cells / Timo Appelhans and Karin Busch -- Analysis of yeast mitochondria by electron microscopy / Ann-Katrin Unger, Stefan Geimer, Max Harner, Walter Neupert, and Benedikt Westermann -- Analysis of mitochondrial membrane protein complexes by electron cryo-tomography / Vicki A.M. Gold, Tobias Brandt, Laetitia Cavellini, Mickael M. Cohen, Raffaele Ieva, and Martin van der Laan -- Assays for Mitophagy in Yeast / Akinori Eiyama and Koji Okamoto -- Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans / Konstantinos Palikaras and Nektarios Tavernarakis -- Assessing mitochondrial unfolded protein response in mammalian cells / Fiona Durand and Nicholas Hoogenraad -- Analysis of mitochondrial RNA-processing defects in patient-derived tissues by qRT-PCR and RNAseq / Robert Kopajtich, Johannes A. Mayr, and Holger Prokisch -- Identification of disease-causing mutations by functional complementation of patient-derived fibroblast cell lines / Laura S. Kremer and Holger Prokisch.
Summary: "This volume compiles a broad range of step-by-step protocols, complementary to the ones published in the first edition of this book, to study various aspects of mitochondrial structure and function in different model organisms, both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitochondria: Practical Protocols, Second Edition aims to be useful for beginners as well as for experienced researchers in the field"--Publisher's description.
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Includes bibliographical references and index.

Guide to computational methods for predicting mitochondrial localization / Su Sun and Bianca H. Habermann -- Isolation of functional mitochondria from cultured cells and mouse tissues / Jennifer Wettmarshausen and Fabiana Perocchi -- Isolation of mitochondria from Saccharomyces cerevisiae / Toshiaki Izawa and Ann-Katrin Unger -- Isolation of contact sites between inner and outer mitochondrial membranes / Max Harner -- Isolation of mitochondria-associated membranes (MAM) from mouse brain tissue / Bernadette Schreiner and Maria Ankarcrona -- Label-free quantitative analysis of mitochondrial proteomes using the multienzyme digestion-filter aided sample preparation (MED-FASP) and "Total Protein Approach" / Jacek R. Wiśniewski -- Quantitative analysis of glycerophospholipids in mitochondria by mass spectrometry / Takashi Tatsuta -- Detection of cysteine redox states in mitochondrial proteins in intact mammalian cells / Markus Habich and Jan Riemer -- Chemical crosslinking in intact mitochondria / Rupa Banerjee, Umut Günsel, and Dejana Mokranjac -- Reconstitution of mitochondrial membrane proteins into nanodiscs by cell-free expression / Ketan Malhotra and Nathan N. Alder -- Detection of dual targeting and dual function of mitochondrial proteins in yeast / Reut Ben-Menachem and Ophry Pines -- Localizing mRNAs encoding mitochondrial proteins in yeast by fluorescence microscopy and subcellular fractionation / Dmitry Zabezhinsky, Hannah Sperber, and Jeffrey E. Gerst -- Assessing mitochondrial bioenergetics in isolated mitochondria from various mouse tissues using seahorse XF96 analyzer / Arcangela Iuso, Birgit Repp, Caroline Biagosch, Caterina Terrile, and Holger Prokisch -- Application of FRET-based biosensor "ATeam" for visualization of ATP levels in the mitochondrial matrix of living mammalian cells / Tomoki Yoshida, Soaad Alfaqaan, Norio Sasaoka, and Hiromi Imamura -- Microplate-based bioluminescence assay of mitochondrial calcium uptake / María Teresa Alonso, Paloma Navas-Navarro, and Javier García-Sancho -- New imaging tools to analyze mitochondrial morphology in Caenorhabditis elegans / Saroj G. Regmi and Stéphane G. Rolland -- Single molecule tracking and localization of mitochondrial protein complexes in live cells / Timo Appelhans and Karin Busch -- Analysis of yeast mitochondria by electron microscopy / Ann-Katrin Unger, Stefan Geimer, Max Harner, Walter Neupert, and Benedikt Westermann -- Analysis of mitochondrial membrane protein complexes by electron cryo-tomography / Vicki A.M. Gold, Tobias Brandt, Laetitia Cavellini, Mickael M. Cohen, Raffaele Ieva, and Martin van der Laan -- Assays for Mitophagy in Yeast / Akinori Eiyama and Koji Okamoto -- Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans / Konstantinos Palikaras and Nektarios Tavernarakis -- Assessing mitochondrial unfolded protein response in mammalian cells / Fiona Durand and Nicholas Hoogenraad -- Analysis of mitochondrial RNA-processing defects in patient-derived tissues by qRT-PCR and RNAseq / Robert Kopajtich, Johannes A. Mayr, and Holger Prokisch -- Identification of disease-causing mutations by functional complementation of patient-derived fibroblast cell lines / Laura S. Kremer and Holger Prokisch.

"This volume compiles a broad range of step-by-step protocols, complementary to the ones published in the first edition of this book, to study various aspects of mitochondrial structure and function in different model organisms, both in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitochondria: Practical Protocols, Second Edition aims to be useful for beginners as well as for experienced researchers in the field"--Publisher's description.

Online resource; title from electronic title page (SpringerLink, viewed July 31, 2018).

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