RNA methylation : methods and protocols / edited by Alexandra Lusser.

Contributor(s): Lusser, Alexandra [editor.]
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 1562.; Springer protocols (Series): Publisher: New York : Humana Press : Springer, [2017]Copyright date: ©2017Description: 1 online resource (xi, 304 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493968077; 1493968076; 149396805X; 9781493968053Subject(s): Methylation | RNA | RNA | Methylation | Medical -- Genetics | Medical geneticsGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: RNA methylation.DDC classification: 572.8/8 LOC classification: QD281.M48Online resources: Click here to access online
Contents:
LC-MS analysis of methylated RNA / Kathrin Thüring, Katharina Schmid, Patrick Keller, and Mark Helm -- Comparative analysis of ribonucleic acid digests (CARD) by mass spectrometry / Mellie June Paulines and Patrick A. Limbach -- Liquid chromatography-mass spectrometry for analysis of RNA adenosine methylation / Bi-Feng Yuan -- Genome-wide location analyses of N6-methyladenosine modifications (m6A-Seq) / Benoit Molinie and Cosmas C. Giallourakis -- Mapping m6A at individual-nucleotide resolution using crosslinking and immunoprecipitation (miCLIP) / Anya V. Grozhik, Bastian Linder, Anthony O. Olarerin-George, and Samie R. Jaffrey -- Detection and quantification of N6-methyladenosine in messenger RNA by TLC / Zsuzsanna Bodi and Rupert G. Fray -- Illustrating the epitranscriptome at nucleotide resolution using methylation-iCLIP (miCLIP) / Harry George, Jernej Ule, and Shobbir Hussain -- Detection of 5-methylcytosine in specific poly(A) RNAs by bisulfite sequencing / Thomas Amort and Alexandra Lusser -- Transcriptome-wide detection of 5-methylcytosine by bisulfite sequencing / Thomas Amort, Xueguang Sun, Daria Khokhlova-Cubberley, and Alexandra Lusser -- Analysis of high-throughput RNA bisulfite sequencing data / Dietmar Rieder and Francesca Finotello -- Statistical methods for transcriptome-wide analysis of RNA methylation by bisulfite sequencing / Brian J. Parker -- High-throughput mapping of 2'-O-me residues in RNA using next-generation sequencing (Illumina RiboMethSeq Protocol) / Virginie Marchand, Lilia Ayadi, Aseel El Hajj, Florence Blanloeil-Oillo, Mark Helm, and Yuri Motorin -- RiboMeth-seq : profiling of 2'-O-me in RNA / Nicolai Krogh, Ulf Birkedal, and Henrik Nielsen -- In silico identification of RNA modifications from high-throughput sequencing data Uuing HAMR / Pavel P. Kuksa, Yuk Yee Leung, Lee E. Vandivier, Zachary Anderson, Brian D. Gregory, and Li-San Wang -- High-throughput small RNA sequencing enhanced by AlkB-facilitated RNA de-methylation (ARM-Seq) / Eva Hrabeta-Robinson, Erin Marcus, Aaron E. Cozen, Eric M. Phizicky, and Todd M. Lowe -- Transcriptome-wide mapping of N1-methyladenosine methylome / Xiaoyu Li, Jinying Peng, and Chengqi Yi -- In vitro assays for RNA methyltransferase activity / Sara Haag, Katherine E. Sloan, Claudia Höbartner, and Markus T. Bohnsack -- Crosslinking methods to identify RNA methyltransferase targets in vivo / Sara Haag, Jens Kretschmer, Katherine E. Sloan, and Markus T. Bohnsack -- Methylated mRNA nucleotides as regulators for ribosomal translation / Thomas P. Hoernes and Matthias D. Erlacher -- Automated chemical solid-phase synthesis and deprotection of 5-hydroxymethylcytosine-containing RNA / Christian Riml and Ronald Micura.
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Includes bibliographical references and index.

LC-MS analysis of methylated RNA / Kathrin Thüring, Katharina Schmid, Patrick Keller, and Mark Helm -- Comparative analysis of ribonucleic acid digests (CARD) by mass spectrometry / Mellie June Paulines and Patrick A. Limbach -- Liquid chromatography-mass spectrometry for analysis of RNA adenosine methylation / Bi-Feng Yuan -- Genome-wide location analyses of N6-methyladenosine modifications (m6A-Seq) / Benoit Molinie and Cosmas C. Giallourakis -- Mapping m6A at individual-nucleotide resolution using crosslinking and immunoprecipitation (miCLIP) / Anya V. Grozhik, Bastian Linder, Anthony O. Olarerin-George, and Samie R. Jaffrey -- Detection and quantification of N6-methyladenosine in messenger RNA by TLC / Zsuzsanna Bodi and Rupert G. Fray -- Illustrating the epitranscriptome at nucleotide resolution using methylation-iCLIP (miCLIP) / Harry George, Jernej Ule, and Shobbir Hussain -- Detection of 5-methylcytosine in specific poly(A) RNAs by bisulfite sequencing / Thomas Amort and Alexandra Lusser -- Transcriptome-wide detection of 5-methylcytosine by bisulfite sequencing / Thomas Amort, Xueguang Sun, Daria Khokhlova-Cubberley, and Alexandra Lusser -- Analysis of high-throughput RNA bisulfite sequencing data / Dietmar Rieder and Francesca Finotello -- Statistical methods for transcriptome-wide analysis of RNA methylation by bisulfite sequencing / Brian J. Parker -- High-throughput mapping of 2'-O-me residues in RNA using next-generation sequencing (Illumina RiboMethSeq Protocol) / Virginie Marchand, Lilia Ayadi, Aseel El Hajj, Florence Blanloeil-Oillo, Mark Helm, and Yuri Motorin -- RiboMeth-seq : profiling of 2'-O-me in RNA / Nicolai Krogh, Ulf Birkedal, and Henrik Nielsen -- In silico identification of RNA modifications from high-throughput sequencing data Uuing HAMR / Pavel P. Kuksa, Yuk Yee Leung, Lee E. Vandivier, Zachary Anderson, Brian D. Gregory, and Li-San Wang -- High-throughput small RNA sequencing enhanced by AlkB-facilitated RNA de-methylation (ARM-Seq) / Eva Hrabeta-Robinson, Erin Marcus, Aaron E. Cozen, Eric M. Phizicky, and Todd M. Lowe -- Transcriptome-wide mapping of N1-methyladenosine methylome / Xiaoyu Li, Jinying Peng, and Chengqi Yi -- In vitro assays for RNA methyltransferase activity / Sara Haag, Katherine E. Sloan, Claudia Höbartner, and Markus T. Bohnsack -- Crosslinking methods to identify RNA methyltransferase targets in vivo / Sara Haag, Jens Kretschmer, Katherine E. Sloan, and Markus T. Bohnsack -- Methylated mRNA nucleotides as regulators for ribosomal translation / Thomas P. Hoernes and Matthias D. Erlacher -- Automated chemical solid-phase synthesis and deprotection of 5-hydroxymethylcytosine-containing RNA / Christian Riml and Ronald Micura.

Online resource; title from PDF title page (SpringerProtocol, viewed March 28, 2017).

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