Promoter associated RNA : methods and protocols / edited by Sara Napoli.

Contributor(s): Napoli, Sara [editor.]
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 1543.Publisher: New York : Humana Press : Springer, [2017]Copyright date: ©2017Description: 1 online resource (x, 287 pages) : illustrations (chiefly color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493967162; 1493967169Subject(s): Non-coding RNA -- Laboratory manuals | RNA -- Laboratory manuals | RNA | Medical -- Genetics | Medical genetics | Non-coding RNA | RNAGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Promoter associated RNA.DDC classification: 572.8/8 LOC classification: QP623Online resources: Click here to access online
Contents:
ChIP-seq for the identification of functional elements in the human genome / Georgi K. Marinov -- Identification of candidate functional elements in the genome from ChIP-seq data / Georgi K. Marinov -- GRO-seq, a tool for identification of transcripts regulating gene expression / Rui Lopes, Reuven Agami, and Gozde Korkmaz -- NanoCAGE : a method for the analysis of coding and noncoding 5'-capped transcriptomes / Stéphane Poulain, Sachi Kato, Ophélie Arnaud, Jean-Étienne Morlighem, Makoto Suzuki, Charles Plessy, and Matthias Harbers -- Deep cap analysis of gene expression (CAGE) : genome-wide identification of promoters, quantification of their activity, and transcriptional network inference / Alexandre Fort and Richard J. Fish -- Deep-RACE : comprehensive search for novel ncRNAs associated to a specific locus / Chiara Pastori, Dmitry Velmeshev, and Veronica Julia Peschansky -- In silico prediction of RNA secondary structure / Fariza Tahi, Van Du T. Tran, and Anouar Boucheham -- Computational prediction of RNA-protein interactions / Carla M. Mann, Usha K. Muppirala, and Drena Dobbs -- Isolation of nuclear RNA-associated protein complexes / Ranveer Singh Jayani, Amanjot Singh, and Dimple Notani -- Identification of long noncoding RNAs associated to human disease susceptibility / Marco Magistri and Dmitry Velmeshev -- Targeting promoter-associated RNAs by siRNAs / Sara Napoli -- RNA-FISH to study regulatory RNA at the site of transcription / Marta Soler, Raquel Boque-Sastre, and Sonia Guil -- Detection and characterization of R loop structures / Raquel Boque-Sastre, Marta Soler, and Sonia Guil -- Induction of transcriptional gene silencing by expression of shRNA directed to c-Myc P2 promoter in hepatocellular carcinoma by tissue-specific virosomal delivery / Mohammad Khalid Zakaria, Debi P. Sarkar, and Parthaprasad Chattopadhyay -- Targeting promoter-associated noncoding RNA in vivo / Gianluca Civenni -- Manipulation of promoter-associated noncoding RNAs in mouse early embryos for controlling sequence-specific epigenetic status / Nobuhiko Hamazaki, Kinichi Nakashima, and Takuya Imamura.
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Includes bibliographical references and index.

ChIP-seq for the identification of functional elements in the human genome / Georgi K. Marinov -- Identification of candidate functional elements in the genome from ChIP-seq data / Georgi K. Marinov -- GRO-seq, a tool for identification of transcripts regulating gene expression / Rui Lopes, Reuven Agami, and Gozde Korkmaz -- NanoCAGE : a method for the analysis of coding and noncoding 5'-capped transcriptomes / Stéphane Poulain, Sachi Kato, Ophélie Arnaud, Jean-Étienne Morlighem, Makoto Suzuki, Charles Plessy, and Matthias Harbers -- Deep cap analysis of gene expression (CAGE) : genome-wide identification of promoters, quantification of their activity, and transcriptional network inference / Alexandre Fort and Richard J. Fish -- Deep-RACE : comprehensive search for novel ncRNAs associated to a specific locus / Chiara Pastori, Dmitry Velmeshev, and Veronica Julia Peschansky -- In silico prediction of RNA secondary structure / Fariza Tahi, Van Du T. Tran, and Anouar Boucheham -- Computational prediction of RNA-protein interactions / Carla M. Mann, Usha K. Muppirala, and Drena Dobbs -- Isolation of nuclear RNA-associated protein complexes / Ranveer Singh Jayani, Amanjot Singh, and Dimple Notani -- Identification of long noncoding RNAs associated to human disease susceptibility / Marco Magistri and Dmitry Velmeshev -- Targeting promoter-associated RNAs by siRNAs / Sara Napoli -- RNA-FISH to study regulatory RNA at the site of transcription / Marta Soler, Raquel Boque-Sastre, and Sonia Guil -- Detection and characterization of R loop structures / Raquel Boque-Sastre, Marta Soler, and Sonia Guil -- Induction of transcriptional gene silencing by expression of shRNA directed to c-Myc P2 promoter in hepatocellular carcinoma by tissue-specific virosomal delivery / Mohammad Khalid Zakaria, Debi P. Sarkar, and Parthaprasad Chattopadhyay -- Targeting promoter-associated noncoding RNA in vivo / Gianluca Civenni -- Manipulation of promoter-associated noncoding RNAs in mouse early embryos for controlling sequence-specific epigenetic status / Nobuhiko Hamazaki, Kinichi Nakashima, and Takuya Imamura.

Online resource; title from PDF title page (SpringerProtocol, viewed April 5, 2017).

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