Genomic mosaicism in neurons and other cell types / editors, José María Frade, Fred H. Gage.
Contributor(s): Frade, José María [editor.] | Gage, F. (Fred) [editor.]Material type: TextSeries: Neuromethods: 131.; Springer protocols (Series): Publisher: New York, NY : Humana Press imprint is published by Springer Nature, Copyright date: ©2017Description: 1 online resource (xvi, 382 pages) : illustrations (some color)Content type: text | still image Media type: computer Carrier type: online resourceISBN: 9781493972807; 1493972804Subject(s): Genomics -- Laboratory manuals | Neurons -- Laboratory manuals | Mosaicism | Neurons -- physiology | Polymorphism, Single Nucleotide | Genomics -- methods | Genomics | NeuronsGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Genomic mosaicism in neurons and other cell types.DDC classification: 576.5 LOC classification: QH447Online resources: Click here to access online
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Includes bibliographical references and index.
Introduction. Principles and approaches for discovery and validation of somatic mosaicism in the human brain / Alexej Abyzov, Alexander E. Urban, Flora M. Vaccarino -- Aneuploidy and ploidy variation. FISH-based assays for detecting genomic (chromosomal) mosaicism in human brain cells / Yuri B. Yurov, Svetlana G. Vorsanova, Ilia V. Soloviev, Alexei M. Ratnikov [and others] -- Flow cytometric and sorting analyses for nuclear DNA content, nucleotide sequencing, and interphase FISH / Gwendolyn E. Kaeser, Jerold Chun -- Flow cytometric quantification, isolation, and subsequent epigenetic analysis of tetraploid neurons / Noelia López-Sánchez, Iris Patiño-Parrado, José Mar̕a Frade -- A cytomic approach towards genomic individuality of neurons / Thomas Arendt, Birgit Belter, Martina K. Brückner, Uwe Ueberham, Markus Morawski [and others] / DNA copy number variation. Single-cell CNV detection in human neuronal nuclei / Margaret B. Wierman, Ian E. Burbulis, William D. Chronister, Stefan Bekiranov [and others] -- Multiple annealing and looping-based amplification cycles (MALBAC) for the analysis of DNA copy number variation / Chenghang Zong -- Competitive PCR for copy number assessment by restricting dNTPs / Luming Zhou, Robert A. Palais, Yotam Ardon, Carl T. Wittwer -- Using cloning to amplify neuronal genomes for whole-genome sequencing and comprehensive mutation detection and validation / Jennifer L. Hazen, Michael A. Duran, Ryan P. Smith, Alberto R. Rodriguez [and others] / LINE-1 retrotransposition. Analysis of LINE-1 retrotransposition in neural progenitor cells and neurons / Angela Macia, Alysson R. Muotri -- Estimation of LINE-1 copy number in the brain tissue and isolated neuronal nuclei / Miki Bundo, Tadafumi Kato, Kazuya Iwamoto -- Analysis of somatic LINE-1 insertions in neurons / Francisco J. Sanchez-Luque, Sandra R. Richardson, Geoffrey J. Faulkner -- Single-cell whole genome amplification and sequencing to study neuronal mosaicism and diversity / Patrick J. Reed, Meiyan Wang, Jennifer A. Erwin, Apuã C.M. Paquola, Fred H. Gage / Genetic and genomic mosaicism in aging and disease. FISH analysis of aging-associated aneuploidy in neurons and nonneuronal brain cells / Grasiella A. Andriani, Cristina Montagna -- Genomic analysis and in vivo functional validation of brain somatic mutations leading to focal cortical malformations / Jae Seok Lim, Jeong Ho Lee -- Using fluorescence in situ hybridization (FISH) analysis to measure chromosome instability and mosaic aneuploidy in neurodegenerative diseases / Julbert Caneus, Antoneta Granic, Heidi J. Chial, Huntington Potter -- Identification of low allele frequency mosaic mutations in Alzheimer disease / Carlo Sala Frigerio, Mark Fiers, Thierry Voet, Bart De Strooper.
"This volume presents methods for the analysis of genomic variability in vertebrate neurons and broadens our knowledge in the ways we understand the brain and its neurons. The chapters in this book are divided into 5 parts, and cover the following topics: principles and approaches for discovery of somatic mosaicism in the brain, aneuploidy and ploidy variation, DNA copy number variation, LINE-1 retrotransposition, and genetic and genomic mosaicism in aging and disease. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Genomic Mosaicism in Neurons and Other Cell Types is a valuable resource for learning about the latest techniques for the analysis of genome and genetic mosaicism in vertebrate neurons"--Publisher's description.
Online resource; title from electronic title page (SpringerLink, viewed September 15, 2017).