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Next generation sequencing : methods and protocols / editors Steven R. Head, Phillip Ordoukhanian, Daniel R. Salomon.

Contributor(s): Head, Steven R [editor.] | Ordoukhanian, Philip [editor.] | Salomon, Daniel R [editor.]
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 1712,Publisher: New York, NY : Humana Press : Springer, [2018]Description: 1 online resource (xiii, 266 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493975143; 1493975145Subject(s): Nucleotide sequence -- Laboratory manuals | High-Throughput Nucleotide Sequencing -- methods | Gene Expression Profiling -- methods | Nucleotide sequenceGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. DDC classification: 572.8/633 LOC classification: QP625.N89Online resources: Click here to access online
Contents:
Integrated polysome profiling and ribosome profiling method to investigate in vivo translatome / Hyun Yong Jin and Changchun Xiao -- Measuring nascent transcripts by nascent-seq / Fei Xavier Chen, Stacy A. Marshall, Yu Deng, and Sun Tianjiao -- Genome-wide copy number alteration detection in preimplantation genetic diagnosis / Lieselot Deleye, Dieter De Coninck, Dieter Deforce, and Filip Van Nieuwerburgh -- Multiplexed targeted sequencing for oxford nanopore MinION : a detailed library preparation procedure / Timokratis Karamitros and Gkikas Magiorkinis -- Hi-Plex for simple, accurate, and cost-effective amplicon-based targeted DNA sequencing / Bernard J. Pope, Fleur Hammet, Tu Nguyen-Dumont, and Daniel J. Park -- ClickSeq : replacing fragmentation and enzymatic ligation with click-chemistry to prevent sequence chimeras / Elizabeth Jaworski and Andrew Routh -- Genome-wide analysis of DNA methylation in single cells using a post-bisulfite adapter tagging approach / Heather J. Lee and Sébastien A. Smallwood -- Sequencing of genomes from environmental single cells / Robert M. Bowers, Janey Lee, and Tanja Woyke -- SNP discovery from single and multiplex genome assemblies of non-model organisms / Phillip A. Morin, Andrew D. Foote, Christopher M. Hill, Benoit Simon-Bouhet, Aimee R. Lang, and Marie Louis -- CleanTag adapters improve small RNA next-generation sequencing library preparation by reducing adapter dimers / Sabrina Shore, Jordana M. Henderson, and Anton P. McCaffrey -- Sampling, extraction, and high-throughput sequencing methods for environmental microbial and viral communities / Pedro J. Torres and Scott T. Kelley -- Bloody primer : analysis of RNA-Seq from tissue admixtures / Casey P. Shannon, Chen Xi Yang, and Scott J. Tebbutt -- Next-generation sequencing of genome-wide CRISPR screens / Edwin H. Yau and Tariq M. Rana -- Gene profiling and T cell receptor sequencing from antigen-specific CD4 T cells / Marie Holt, Anne Costanzo, Louis Gioia, Brian Abe, Andrew I. Su, and Luc Teyton -- Investigate global chromosomal interaction by Hi-C in human naive CD4 T cells / Xiangzhi Meng, Nicole Riley, Ryan Thompson, and Siddhartha Sharma -- Primer extension, capture, and on-bead cDNA ligation : an efficient RNAseq library prep method for determining reverse transcription termination sites / Phillip Ordoukhanian, Jessica Nichols, and Steven R. Head.
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Includes bibliographical references and index.

Integrated polysome profiling and ribosome profiling method to investigate in vivo translatome / Hyun Yong Jin and Changchun Xiao -- Measuring nascent transcripts by nascent-seq / Fei Xavier Chen, Stacy A. Marshall, Yu Deng, and Sun Tianjiao -- Genome-wide copy number alteration detection in preimplantation genetic diagnosis / Lieselot Deleye, Dieter De Coninck, Dieter Deforce, and Filip Van Nieuwerburgh -- Multiplexed targeted sequencing for oxford nanopore MinION : a detailed library preparation procedure / Timokratis Karamitros and Gkikas Magiorkinis -- Hi-Plex for simple, accurate, and cost-effective amplicon-based targeted DNA sequencing / Bernard J. Pope, Fleur Hammet, Tu Nguyen-Dumont, and Daniel J. Park -- ClickSeq : replacing fragmentation and enzymatic ligation with click-chemistry to prevent sequence chimeras / Elizabeth Jaworski and Andrew Routh -- Genome-wide analysis of DNA methylation in single cells using a post-bisulfite adapter tagging approach / Heather J. Lee and Sébastien A. Smallwood -- Sequencing of genomes from environmental single cells / Robert M. Bowers, Janey Lee, and Tanja Woyke -- SNP discovery from single and multiplex genome assemblies of non-model organisms / Phillip A. Morin, Andrew D. Foote, Christopher M. Hill, Benoit Simon-Bouhet, Aimee R. Lang, and Marie Louis -- CleanTag adapters improve small RNA next-generation sequencing library preparation by reducing adapter dimers / Sabrina Shore, Jordana M. Henderson, and Anton P. McCaffrey -- Sampling, extraction, and high-throughput sequencing methods for environmental microbial and viral communities / Pedro J. Torres and Scott T. Kelley -- Bloody primer : analysis of RNA-Seq from tissue admixtures / Casey P. Shannon, Chen Xi Yang, and Scott J. Tebbutt -- Next-generation sequencing of genome-wide CRISPR screens / Edwin H. Yau and Tariq M. Rana -- Gene profiling and T cell receptor sequencing from antigen-specific CD4 T cells / Marie Holt, Anne Costanzo, Louis Gioia, Brian Abe, Andrew I. Su, and Luc Teyton -- Investigate global chromosomal interaction by Hi-C in human naive CD4 T cells / Xiangzhi Meng, Nicole Riley, Ryan Thompson, and Siddhartha Sharma -- Primer extension, capture, and on-bead cDNA ligation : an efficient RNAseq library prep method for determining reverse transcription termination sites / Phillip Ordoukhanian, Jessica Nichols, and Steven R. Head.

Online resource; title from PDF title page (SpringerProtocol, viewed December 13, 2017).

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