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Mitochondrial bioenergetics : methods and protocols / edited by Carlos M. Palmeira and António J. Moreno.

Contributor(s): Palmeira, Carlos M [editor.] | Moreno, António J [editor.]Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1782.Publisher: New York : Humana Press ; Springer, [2018]Copyright date: ©2018Edition: Second editionDescription: 1 online resource (xi, 422 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493978311; 1493978314Subject(s): Mitochondrial pathology -- Laboratory manuals | Bioenergetics -- Laboratory manuals | Mitochondria -- physiology | Mitochondria -- genetics | Mitochondrial Proteins | Models, Biological | Cellular biology (cytology) | Science -- Life Sciences -- Biology -- Molecular Biology | Bioenergetics | Mitochondrial pathologyGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Mitochondrial bioenergetics.DDC classification: 616.07 LOC classification: RB147.5NLM classification: QU 25Online resources: Click here to access online
Contents:
Overview of mitochondrial bioenergetics / Vitor M. C. Madeira -- Evaluation of respiration with clark-type electrode in isolated mitochondria and permeabilized animal cells / Ana M. Silva and Paulo J. Oliveira -- High-resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated Mitochondria / Carolina Doerrier, Luiz F. Garcia-Souza, Gerhard Krumschnabel, Yvonne Wohlfarter, Andras T. Meszaros, and Erich Gnaiger -- High-throughput analysis of mitochondrial oxygen consumption / James Hynes, Rachel L. Swiss, and Yvonne Will -- Modulation of cellular respiration by endogenously produced nitric oxide in rat hippocampal slices / Ana Ledo, Rui M. Barbosa, and Joao Laranjinha -- Mitochondrial membrane potential (??) fluctuations associated with the metabolic states of mitochondria / Joao Soeiro Teodoro, Carlos Marques Palmeira, and Anabela Pinto Rolo -- Fluorescence measurement of mitochondrial membrane potential changes in cultured cells / David G. Nicholls -- Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production / Timea Koml odi, Ondrej Sobotka, Gerhard Krumschnabel, Nicole Bezuidenhout, Elisabeth Hiller, Carolina Doerrier, and Erich Gnaiger -- Measurement of proton leak in isolated mitochondria / Charles Affourtit, Hoi-Shan Wong, and Martin D. Brand -- Imaging mitochondrial calcium fluxes with fluorescent probes and single- or two-photon confocal microscopy / Sean M. Davidson and Michael R. Duchen -- Mitochondrial permeability transition pore and calcium handling / Randi J. Parks, Elizabeth Murphy, and Julia C. Liu -- Redox equivalents and mitochondrial bioenergetics / James R. Roede, Young-Mi Go, and Dean P. Jones -- Mitochondrial bioenergetics by 13C-NMR isotopomer analysis / Rui A. Carvalho and Ivana Jarak -- Computational modeling of mitochondrial function from a systems biology perspective / Sonia Cortassa, Steven J. Sollott, and Miguel A. Aon -- Monitoring the mitochondrial dynamics in mammalian cells / Luca Simula and Silvia Campello -- Plate-based measurement of superoxide and hydrogen peroxide production by isolated mitochondria / Hoi-Shan Wong, Pierre-Axel Monternier, Adam L. Orr, and Martin D. Brand -- Plate-based measurement of respiration by isolated mitochondria / Shona A. Mookerjee, Casey L. Quinlan, Hoi-Shan Wong, Pratiksha Dighe, and Martin D. Brand -- Detection of iron depletion- and hypoxia-induced mitophagy in mammalian cells / Shun-Ichi Yamashita and Tomotake Kanki -- Importance of calcium ions for determining mitochondrial glycerol-3-phosphate dehydrogenase activity when measuring uncoupling protein 1 (UCP1) function in mitochondria isolated from brown adipose tissue / Kieran J. Clarke and Richard K. Porter -- Isolation and analysis of mitochondrial small RNAs from rat liver tissue and HepG2 cells / Julian Geiger and Louise T. Dalgaard -- Imaging of mitochondrial pH using SNARF-1 / Venkat K. Ramshesh and John J. Lemasters -- Relation between mitochondrial membrane potential and ROS formation / Jan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski, and Mariusz R. Wieckowski -- Assessing spatiotemporal and functional organization of mitochondrial networks / Felix T. Kurz, Miguel A. Aon, Brian O'Rourke, and Antonis A. Armoundas -- Measurement of mitochondrial ROS formation / Soni Deshwal, Salvatore Antonucci, Nina Kaludercic, and Fabio Di Lisa.
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Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerProtocols, viewed June 1, 2018)

Overview of mitochondrial bioenergetics / Vitor M. C. Madeira -- Evaluation of respiration with clark-type electrode in isolated mitochondria and permeabilized animal cells / Ana M. Silva and Paulo J. Oliveira -- High-resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated Mitochondria / Carolina Doerrier, Luiz F. Garcia-Souza, Gerhard Krumschnabel, Yvonne Wohlfarter, Andras T. Meszaros, and Erich Gnaiger -- High-throughput analysis of mitochondrial oxygen consumption / James Hynes, Rachel L. Swiss, and Yvonne Will -- Modulation of cellular respiration by endogenously produced nitric oxide in rat hippocampal slices / Ana Ledo, Rui M. Barbosa, and Joao Laranjinha -- Mitochondrial membrane potential (??) fluctuations associated with the metabolic states of mitochondria / Joao Soeiro Teodoro, Carlos Marques Palmeira, and Anabela Pinto Rolo -- Fluorescence measurement of mitochondrial membrane potential changes in cultured cells / David G. Nicholls -- Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production / Timea Koml odi, Ondrej Sobotka, Gerhard Krumschnabel, Nicole Bezuidenhout, Elisabeth Hiller, Carolina Doerrier, and Erich Gnaiger -- Measurement of proton leak in isolated mitochondria / Charles Affourtit, Hoi-Shan Wong, and Martin D. Brand -- Imaging mitochondrial calcium fluxes with fluorescent probes and single- or two-photon confocal microscopy / Sean M. Davidson and Michael R. Duchen -- Mitochondrial permeability transition pore and calcium handling / Randi J. Parks, Elizabeth Murphy, and Julia C. Liu -- Redox equivalents and mitochondrial bioenergetics / James R. Roede, Young-Mi Go, and Dean P. Jones -- Mitochondrial bioenergetics by 13C-NMR isotopomer analysis / Rui A. Carvalho and Ivana Jarak -- Computational modeling of mitochondrial function from a systems biology perspective / Sonia Cortassa, Steven J. Sollott, and Miguel A. Aon -- Monitoring the mitochondrial dynamics in mammalian cells / Luca Simula and Silvia Campello -- Plate-based measurement of superoxide and hydrogen peroxide production by isolated mitochondria / Hoi-Shan Wong, Pierre-Axel Monternier, Adam L. Orr, and Martin D. Brand -- Plate-based measurement of respiration by isolated mitochondria / Shona A. Mookerjee, Casey L. Quinlan, Hoi-Shan Wong, Pratiksha Dighe, and Martin D. Brand -- Detection of iron depletion- and hypoxia-induced mitophagy in mammalian cells / Shun-Ichi Yamashita and Tomotake Kanki -- Importance of calcium ions for determining mitochondrial glycerol-3-phosphate dehydrogenase activity when measuring uncoupling protein 1 (UCP1) function in mitochondria isolated from brown adipose tissue / Kieran J. Clarke and Richard K. Porter -- Isolation and analysis of mitochondrial small RNAs from rat liver tissue and HepG2 cells / Julian Geiger and Louise T. Dalgaard -- Imaging of mitochondrial pH using SNARF-1 / Venkat K. Ramshesh and John J. Lemasters -- Relation between mitochondrial membrane potential and ROS formation / Jan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski, and Mariusz R. Wieckowski -- Assessing spatiotemporal and functional organization of mitochondrial networks / Felix T. Kurz, Miguel A. Aon, Brian O'Rourke, and Antonis A. Armoundas -- Measurement of mitochondrial ROS formation / Soni Deshwal, Salvatore Antonucci, Nina Kaludercic, and Fabio Di Lisa.

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Mitochondrial bioenergetics : ©2012
Mitochondrial bioenergetics : ©2012

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