Cellular senescence : methods and protocols / edited by Marco Demaria.

Contributor(s): Demaria, Marco [editor.]
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 1896.Publisher: New York, NY : Humana Press : Springer, [2018]Copyright date: ©2019Description: 1 online resource (xii, 253 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493989317; 1493989316Subject(s): Cells -- Aging -- Laboratory manuals | Cellular Senescence | Cells -- AgingGenre/Form: Electronic books. | Laboratory Manual. | Laboratory manuals. Additional physical formats: Print version:: Cellular senescence.DDC classification: 571.6 LOC classification: QH608Online resources: Click here to access online
Contents:
Detecting cellular senescence in reprogramming / Coralie Cazin, Mathieu von Joest, and Han Li -- DNA damage in situ ligation followed by proximity ligation assay (DI-PLA) / Alessandro Galbiati and Fabrizio d'Adda di Fagagna -- Reactive oxygen species detection in senescent cells / Stella Victorelli and Joa˜o F. Passos -- Cellular identification and quantification of senescence-associated ?-galactosidase activity in vivo / Bennett G. Childs, Tyler J. Bussian, and Darren J. Baker -- Relative human telomere length quantification by real-time PCR / A. Vasilishina, A. Kropotov, I. Spivak, and A. Bernadotte -- Assessing functional roles of the senescence-associated secretory phenotype (SASP) / Nicolas Malaquin, Véronique Tu, and Francis Rodier -- Measuring the inflammasome in oncogene-induced / Irene Fernández-Duran, Nú ria Tarrats, Priya Hari, and Juan Carlos Acosta -- Alarmin detection in senescent cells / Dong Eun Kim and Albert R. Davalos -- IMR90 ER:RAS: a cell model of oncogene-induced senescence / Andrew J. Innes and Jesú s Gil -- Genotoxic stress-induced senescence / Dorothy N. Y. Fan and Clemens A. Schmitt -- Multiparametric assay to evaluate senescent cells / Hilah Gal, Ziv Porat, and Valery Krizhanovsky -- Novel quantitative method for the detection of lipofuscin, the main by-product of cellular senescence, in fluids / Sophia V. Rizou, Konstantinos Evangelou, Vassilios Myrianthopoulos, Iordanis Mourouzis, Sophia Havaki, Aikaterini Athanasiou, Panagiotis V. S. Vasileiou, Aggelos Margetis, Athanassios Kotsinas, Nikolaos G. Kastrinakis, Petros Sfikakis, Paul Townsend, Emmanuel Mikros, Constantinos Pantos, and Vassilis G. Gorgoulis -- Measurement of metabolite changes in senescent cells by mass spectrometry / Christopher D. Wiley, Sonnet Davis, and Arvind Ramanathan -- Quantification of autophagy during senescence / Joon Tae Park, Young-Sam Lee, and Sang Chul Park -- Quantification of aneuploidy in mammalian systems / Hilda van den Bos, Bjorn Bakker, Aaron Taudt, Victor Guryev, Maria Colomé-Tatché, Peter M. Lansdorp, Floris Foijer, and Diana C. J. Spierings -- Identification of genomic alterations through multilevel DNA structural analysis / Ryan K. Shultzaberger and John Dresios -- Mouse models of accelerated cellular senescence / Matthew J. Yousefzadeh, Kendra I. Melos, Luise Angelini, Christin E. Burd, Paul D. Robbins, and Laura J. Niedernhofer -- Methods to quantify the NF-?B pathway during senescence / Lei Zhang, Jing Zhao, Aditi Gurkar, Laura J. Niedernhofer, and Paul D. Robbins.
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Includes bibliographical references and index.

Online resource; title from PDF title page (SpringerLink, viewed November 26, 2018).

880-01 Detecting cellular senescence in reprogramming / Coralie Cazin, Mathieu von Joest, and Han Li -- DNA damage in situ ligation followed by proximity ligation assay (DI-PLA) / Alessandro Galbiati and Fabrizio d'Adda di Fagagna -- Reactive oxygen species detection in senescent cells / Stella Victorelli and Joa˜o F. Passos -- Cellular identification and quantification of senescence-associated ?-galactosidase activity in vivo / Bennett G. Childs, Tyler J. Bussian, and Darren J. Baker -- Relative human telomere length quantification by real-time PCR / A. Vasilishina, A. Kropotov, I. Spivak, and A. Bernadotte -- Assessing functional roles of the senescence-associated secretory phenotype (SASP) / Nicolas Malaquin, Véronique Tu, and Francis Rodier -- Measuring the inflammasome in oncogene-induced / Irene Fernández-Duran, Nú ria Tarrats, Priya Hari, and Juan Carlos Acosta -- Alarmin detection in senescent cells / Dong Eun Kim and Albert R. Davalos -- IMR90 ER:RAS: a cell model of oncogene-induced senescence / Andrew J. Innes and Jesú s Gil -- Genotoxic stress-induced senescence / Dorothy N. Y. Fan and Clemens A. Schmitt -- Multiparametric assay to evaluate senescent cells / Hilah Gal, Ziv Porat, and Valery Krizhanovsky -- Novel quantitative method for the detection of lipofuscin, the main by-product of cellular senescence, in fluids / Sophia V. Rizou, Konstantinos Evangelou, Vassilios Myrianthopoulos, Iordanis Mourouzis, Sophia Havaki, Aikaterini Athanasiou, Panagiotis V. S. Vasileiou, Aggelos Margetis, Athanassios Kotsinas, Nikolaos G. Kastrinakis, Petros Sfikakis, Paul Townsend, Emmanuel Mikros, Constantinos Pantos, and Vassilis G. Gorgoulis -- Measurement of metabolite changes in senescent cells by mass spectrometry / Christopher D. Wiley, Sonnet Davis, and Arvind Ramanathan -- Quantification of autophagy during senescence / Joon Tae Park, Young-Sam Lee, and Sang Chul Park -- Quantification of aneuploidy in mammalian systems / Hilda van den Bos, Bjorn Bakker, Aaron Taudt, Victor Guryev, Maria Colomé-Tatché, Peter M. Lansdorp, Floris Foijer, and Diana C. J. Spierings -- Identification of genomic alterations through multilevel DNA structural analysis / Ryan K. Shultzaberger and John Dresios -- Mouse models of accelerated cellular senescence / Matthew J. Yousefzadeh, Kendra I. Melos, Luise Angelini, Christin E. Burd, Paul D. Robbins, and Laura J. Niedernhofer -- Methods to quantify the NF-?B pathway during senescence / Lei Zhang, Jing Zhao, Aditi Gurkar, Laura J. Niedernhofer, and Paul D. Robbins.

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