Guide to yeast genetics and molecular biology / edited by Christine Guthrie, Gerald R. Fink.

Contributor(s): Guthrie, Christine [editor.] | Fink, Gerald R [editor.]
Material type: TextTextSeries: Methods in enzymology: v. 194.Publisher: San Diego : Academic Press, ©1991Description: 1 online resource (xxxvii, 933 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 0080571115; 9780080571119; 0121820955; 9780121820954Subject(s): Yeast fungi -- Genetics | Yeast fungi -- Cytology | Genetic Techniques | Molecular Biology | Yeasts -- genetics | Levures (Botanique) -- Génétique | Biologie moléculaire | NATURE -- Plants -- Mushrooms | Enzymes | Biochimie | Yeast fungi -- Cytology | Yeast fungi -- Genetics | Gist | Genetica | Moleculaire biologie | Genetik | Hefeartige Pilze | Methode | Molekularbiologie | Saccharomycetaceae | Aufsatzsammlung | Fungi GeneticsGenre/Form: Electronic books. Additional physical formats: Print version:: Guide to yeast genetics and molecular biology.DDC classification: 579.5/63 LOC classification: QP601Other classification: 42.15 | VK 8700 | WC 4355 | WD 5050 | BIO 180f | BIO 220f | BIO 325f | CHE 885f Online resources: Click here to access online
Contents:
Section I. Basic methods in yeast genetics -- Section II. Cloning and recombinant DNA -- Section III. Making mutants -- Section IV. Biochemistry of gene expression -- Section V. Cell biology -- Section VI. Fission yeast -- Section VII. Appendix.
Action note: digitized 2011 committed to preserveSummary: Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein.
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Includes bibliographical references and indexes.

Section I. Basic methods in yeast genetics -- Section II. Cloning and recombinant DNA -- Section III. Making mutants -- Section IV. Biochemistry of gene expression -- Section V. Cell biology -- Section VI. Fission yeast -- Section VII. Appendix.

Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein.

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