Nucleosomes. Volume 170 / edited by Paul M. Wassarman, Roger D. Kornberg.

Contributor(s): Wassarman, Paul M [editor.] | Kornberg, Roger D [editor.]
Material type: TextTextSeries: Methods in enzymology: volume 170.Publisher: San Diego : Academic Press, ©1989Description: 1 online resource (xxix, 683 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 0121820718; 9780121820718Subject(s): Chromosomal proteins | Chromatin | Histones | Nucleosomes -- chemistry | Chromatine | Nucléosomes | Chromatin | Chromosomal proteins | Histones | Nucleosom | Aufsatzsammlung | Nucleosomen | Nucleosom | AufsatzsammlungGenre/Form: Electronic books. Additional physical formats: Print version:: Nucleosomes.DDC classification: 572.87 LOC classification: QP601 | .C733 v. 170Online resources: Click here to access online
Contents:
Preparation of nucleosomes and chromatin / Roger D. Kornberg, Jan W. Lapointe, Yahli Lorch -- Preparation of simian virus 40 minichromosomes / Pierre Oudet, Etienne Weiss, Evelyne Regnier -- Isolation of yeast plasmid chromatin / Ann Dean, David S. Pederson, Robert T. Simpson -- Affinity isolation of replicating simian virus 40 chromosomes / Timothy M. Herman -- Sucrose gradient techniques and applications to nucleosome structure / Hans Noll, Markus Noll -- Electrophoretic analyses of nucleosomes and other protein-DNA complexes / Sue-Ying Huang, William T. Garrard -- Electron microscopy of chromatin / Jose M. Sogo, Fritz Thoma -- Electron microscopy of simian virus 40 minichromosomes / Pierre Oudet, Patrick Schultz, Jean-Claude Homo, Pierre Colin -- Immunoelectron microscopy of nucleosomes / Christopher L. F. Woodcock -- Use of fluorescent probes to study nucleosomes / Joan-Ramon Daban, Charles R. Cantor -- Preparation and application of immunological probes for nucleosomes / Michael Bustin -- Use of antihistone antibodies with nucleosomes / Sylviane Muller, Marc H.V. Van Regenmortel -- Digestion of nucleosomes with deoxyribonucleases I and II / Leonard C. Lutter -- Analysis of hypersensitive sites in chromatin / Carl Wu -- Application of nucleases to visualizing chromatin organization at replication forks / Michael E. Cusick, Paul M. Wassarman, Melvin L. Depamphilis -- Nuclease digestion of transcriptionally active chromatin / Maria Bellard, Guy Dretzen, Angela Giangrande, Philippe Ramain -- Analysis of RNA polymerase III transcription in Vitro using chromatin and cloned gene templates / Joel M. Gottesfeld -- Nonenzymatic cleavage of chromatin / Iain L. Cartwright, Sarah C. R. Elgin -- Chemical radiolabeling of lysines that interact strongly with DNA in chromatin / Jean O. Thomas -- Mapping DNA-protein interactions by cross-linking / A. D. Mirzabekov, S. G. Bavykin, A. V. Belyavsky, V. L. Karpov, O.V. Preobrazhenskaya, V. V. Shick, K. K. Ebralidse -- Analysis of nucleosome positioning by indirect end-labeling and molecular cloning / S. A. Nedospasov, A. N. Shakhov, G. P. Georgiev -- Crystallization of nucleosome core particles / D. Rhodes, R.S. Brown, A. Klug -- Isolation and characterization of histones / C. von Holt, W. F. Brandt, H. J. Greyling, G. G. Lindsey, J. D. Retief, J. de A. Rodrigues, S. Schwager, B.T. Sewell -- Purification and analysis of H1 histones / R. David Cole -- Analysis of histone subtypes and their modified forms by polyacrylamide gel electrophoresis / Ronald W. Lennox, Leonard H. Cohen -- Chemical cross-linking of histones / Jean O. Thomas -- Assembly of nucleosomes and chromatin in Vitro / Daniela Rhodes, Ronald A. Laskey -- Reconstitution of chromatin from purified components / Arnold Stein -- Assembly of chromatin with oocyte extracts / Akiko Shimamura, Bret Jessee, Abraham Worcel -- Purification of Xenopus egg nucleoplasmin and its use in chromatin assembly in Vitro / Linda Sealy, Richard R. Burgess, Matt Cotten, Roger Chalkley -- Analysis of nucleosome positioning by in Vitro reconstitution / Bernd Neubauer, Wolfram Hörz.
Summary: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
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Includes bibliographical references and indexes.

Preparation of nucleosomes and chromatin / Roger D. Kornberg, Jan W. Lapointe, Yahli Lorch -- Preparation of simian virus 40 minichromosomes / Pierre Oudet, Etienne Weiss, Evelyne Regnier -- Isolation of yeast plasmid chromatin / Ann Dean, David S. Pederson, Robert T. Simpson -- Affinity isolation of replicating simian virus 40 chromosomes / Timothy M. Herman -- Sucrose gradient techniques and applications to nucleosome structure / Hans Noll, Markus Noll -- Electrophoretic analyses of nucleosomes and other protein-DNA complexes / Sue-Ying Huang, William T. Garrard -- Electron microscopy of chromatin / Jose M. Sogo, Fritz Thoma -- Electron microscopy of simian virus 40 minichromosomes / Pierre Oudet, Patrick Schultz, Jean-Claude Homo, Pierre Colin -- Immunoelectron microscopy of nucleosomes / Christopher L. F. Woodcock -- Use of fluorescent probes to study nucleosomes / Joan-Ramon Daban, Charles R. Cantor -- Preparation and application of immunological probes for nucleosomes / Michael Bustin -- Use of antihistone antibodies with nucleosomes / Sylviane Muller, Marc H.V. Van Regenmortel -- Digestion of nucleosomes with deoxyribonucleases I and II / Leonard C. Lutter -- Analysis of hypersensitive sites in chromatin / Carl Wu -- Application of nucleases to visualizing chromatin organization at replication forks / Michael E. Cusick, Paul M. Wassarman, Melvin L. Depamphilis -- Nuclease digestion of transcriptionally active chromatin / Maria Bellard, Guy Dretzen, Angela Giangrande, Philippe Ramain -- Analysis of RNA polymerase III transcription in Vitro using chromatin and cloned gene templates / Joel M. Gottesfeld -- Nonenzymatic cleavage of chromatin / Iain L. Cartwright, Sarah C. R. Elgin -- Chemical radiolabeling of lysines that interact strongly with DNA in chromatin / Jean O. Thomas -- Mapping DNA-protein interactions by cross-linking / A. D. Mirzabekov, S. G. Bavykin, A. V. Belyavsky, V. L. Karpov, O.V. Preobrazhenskaya, V. V. Shick, K. K. Ebralidse -- Analysis of nucleosome positioning by indirect end-labeling and molecular cloning / S. A. Nedospasov, A. N. Shakhov, G. P. Georgiev -- Crystallization of nucleosome core particles / D. Rhodes, R.S. Brown, A. Klug -- Isolation and characterization of histones / C. von Holt, W. F. Brandt, H. J. Greyling, G. G. Lindsey, J. D. Retief, J. de A. Rodrigues, S. Schwager, B.T. Sewell -- Purification and analysis of H1 histones / R. David Cole -- Analysis of histone subtypes and their modified forms by polyacrylamide gel electrophoresis / Ronald W. Lennox, Leonard H. Cohen -- Chemical cross-linking of histones / Jean O. Thomas -- Assembly of nucleosomes and chromatin in Vitro / Daniela Rhodes, Ronald A. Laskey -- Reconstitution of chromatin from purified components / Arnold Stein -- Assembly of chromatin with oocyte extracts / Akiko Shimamura, Bret Jessee, Abraham Worcel -- Purification of Xenopus egg nucleoplasmin and its use in chromatin assembly in Vitro / Linda Sealy, Richard R. Burgess, Matt Cotten, Roger Chalkley -- Analysis of nucleosome positioning by in Vitro reconstitution / Bernd Neubauer, Wolfram Hörz.

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

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