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Advanced bacterial genetics : Use of transposons and phage for genomic engineering / edited by Kelly T. Hughes, Stanley R. Maloy.

Contributor(s): Hughes, Kelly Thomas [editor.] | Maloy, Stanley R [editor.]
Material type: TextTextSeries: Methods in enzymology: v. 421.Publisher: San Diego, California : Elsevier Academic Press, ©2007Description: 1 online resource (xxxiv, 284 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9780080475103; 0080475108; 0123737494; 9780123737496; 1281003646; 9781281003645Other title: Use of transposons and phage for genomic engineeringSubject(s): Bacterial genetics | Transposons | Bacteriophages | Bacteria -- genetics | DNA, Bacterial | SCIENCE -- Life Sciences -- Biochemistry | Bacterial genetics | Bacteriophages | Transposons | Genetische manipulatie | Bacteriën | Transposonen | Bacteriofagen | Genoom | Genomica | Mutagenesis | genetica | genetics | bacteria | transposons | transposable elements | faagvectoren | phage vectors | genetische modificatie | genetic engineering | genexpressieanalyse | genomics | Genetic Engineering | Genetische modificatieGenre/Form: Electronic books. Additional physical formats: Print version:: Advanced bacterial genetics.DDC classification: 572/.7 LOC classification: QP601 | .M49 vol. 421Other classification: 42.33 | 35.71 Online resources: Click here to access online
Contents:
Strain collections and genetic nomenclature / Stanley R. Maloy and Kelly T. Hughes -- Use of antibiotic-resistant transposons for mutagenesis / Stanley R. Maloy -- In vivo mutagenesis using EZ-Tn5 / John R. Kirby -- Identification of essential genes in bacteria / David R. Hillyard and Michael J. Reed -- Isolation of transposon insertions / Stanley R. Maloy -- Localized mutagenesis / Stanley R. Maloy -- Generation of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons / Kelly T. Hughes -- Target-directed proteolysis in vivo / Markus Eser [and others] -- Sets of transposon-generated sequence-tagged mutants for structure-function analysis and engineering / Beth Traxler and Eliora Gachelet -- Using genomic microarrays to study insertional/transposon mutant libraries / David N. Baldwin and Nina R. Salama -- Screening transposon mutant libraries using full-genome oligonucleotide microarrays / Kelly M. Winterberg and William S. Reznikoff -- Creating recombination-activated genes and sequence-defined mutant libraries using transposons / Larry Gallagher [and others] -- Use of operon and gene fusions to study gene regulation in Salmonella / Kelly T. Hughes and Stanley R. Maloy -- Genomic screening for regulatory genes using the T-POP transposon / Changhan Lee [and others] -- Recombineering: In vivo genetic engineering in E. coli, S. enterica, and beyond / James A. Sawitzke [and others] -- [lambda]-red genetic engineering in Salmonella enterica serovar Typhimurium / Joyce E. Karlinsey -- Probing nucleoid structure in bacteria using phage lambda integrase-mediated chromosome rearrangements / Nathalie Garcia-Russell, Samantha S. Orchard, and Anca M. Segall -- Dissecting nucleic acid-protein interactions using challenge phage / Stanley R. Maloy and Jeffrey Gardner -- Mud-P22 / Jenny A. Crain and Stanley R. Maloy -- Phage metagenomics / Veronica Casas and Forest Rowher.
Summary: A volume of indispensable methods for the use of transposons and phage in genomic engineering.
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Includes bibliographical references and indexes.

Strain collections and genetic nomenclature / Stanley R. Maloy and Kelly T. Hughes -- Use of antibiotic-resistant transposons for mutagenesis / Stanley R. Maloy -- In vivo mutagenesis using EZ-Tn5 / John R. Kirby -- Identification of essential genes in bacteria / David R. Hillyard and Michael J. Reed -- Isolation of transposon insertions / Stanley R. Maloy -- Localized mutagenesis / Stanley R. Maloy -- Generation of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons / Kelly T. Hughes -- Target-directed proteolysis in vivo / Markus Eser [and others] -- Sets of transposon-generated sequence-tagged mutants for structure-function analysis and engineering / Beth Traxler and Eliora Gachelet -- Using genomic microarrays to study insertional/transposon mutant libraries / David N. Baldwin and Nina R. Salama -- Screening transposon mutant libraries using full-genome oligonucleotide microarrays / Kelly M. Winterberg and William S. Reznikoff -- Creating recombination-activated genes and sequence-defined mutant libraries using transposons / Larry Gallagher [and others] -- Use of operon and gene fusions to study gene regulation in Salmonella / Kelly T. Hughes and Stanley R. Maloy -- Genomic screening for regulatory genes using the T-POP transposon / Changhan Lee [and others] -- Recombineering: In vivo genetic engineering in E. coli, S. enterica, and beyond / James A. Sawitzke [and others] -- [lambda]-red genetic engineering in Salmonella enterica serovar Typhimurium / Joyce E. Karlinsey -- Probing nucleoid structure in bacteria using phage lambda integrase-mediated chromosome rearrangements / Nathalie Garcia-Russell, Samantha S. Orchard, and Anca M. Segall -- Dissecting nucleic acid-protein interactions using challenge phage / Stanley R. Maloy and Jeffrey Gardner -- Mud-P22 / Jenny A. Crain and Stanley R. Maloy -- Phage metagenomics / Veronica Casas and Forest Rowher.

Print version record.

A volume of indispensable methods for the use of transposons and phage in genomic engineering.

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