Cilia and flagella / edited by William Dentler and George Witman.

Contributor(s): Dentler, William [editor.] | Witman, George [editor.] | American Society for Cell Biology
Material type: TextTextSeries: Methods in cell biology: v. 47.Publisher: San Diego : Academic Press, [1995]Copyright date: ©1995Description: 1 online resource (xxxv, 603 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9780080859453; 0080859453; 0125641486; 9780125641487; 1281514144; 9781281514141Subject(s): Cilia and ciliary motion -- Laboratory manuals | Flagella (Microbiology) -- Laboratory manuals | Cilia -- physiology | Cilia -- ultrastructure | Flagella -- physiology | Flagella -- ultrastructure | Cils vibratiles | Flagellés | SCIENCE -- Life Sciences -- Cell Biology | Cilia and ciliary motion | Flagella (Microbiology) | Trilharen | Flagellen | Zilie | Methode | Geißel Biologie | Experiment | CytologyGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Cilia and flagella.DDC classification: 574.87/64 LOC classification: QP310.C5 | C55 1995ebOther classification: 42.15 | WC 5100 | WE 3750 Online resources: Click here to access online
Contents:
pt. 1. Isolation of cilia and flagella -- pt. 2. Culture and isolation of ciliated epithelial cells and cilia -- pt. 3. General methods -- pt. 4. Structure -- pt. 5. Motors and motion analysis -- pt. 6. Cytoskeletal preparations -- pt. 7. Flagellar fractionation and membrane characterization -- pt. 8. Assay and isolation of flagellar enzymes -- pt. 9. Genetics and molecular tools for the analysis of cilia and flagella.
Summary: Cilia and Flagella presents protocols accessible to all individuals working with eukaryotic cilia and flagella. These recipes delineate laboratory methods and reagents, as well as critical steps and pitfalls of the procedures. The volume covers the roles of cilia and flagella in cell assembly and motility, the cell cycle, cell-cell recognition and other sensory functions, as well as human diseases and disorders. Students, researchers, professors, and clinicians should find the book's combination of "classic" and innovative techniques essential to the study of cilia and flagella.
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"Prepared under the auspices of the American Society for Cell Biology."

Includes bibliographical references and index.

pt. 1. Isolation of cilia and flagella -- pt. 2. Culture and isolation of ciliated epithelial cells and cilia -- pt. 3. General methods -- pt. 4. Structure -- pt. 5. Motors and motion analysis -- pt. 6. Cytoskeletal preparations -- pt. 7. Flagellar fractionation and membrane characterization -- pt. 8. Assay and isolation of flagellar enzymes -- pt. 9. Genetics and molecular tools for the analysis of cilia and flagella.

Cilia and Flagella presents protocols accessible to all individuals working with eukaryotic cilia and flagella. These recipes delineate laboratory methods and reagents, as well as critical steps and pitfalls of the procedures. The volume covers the roles of cilia and flagella in cell assembly and motility, the cell cycle, cell-cell recognition and other sensory functions, as well as human diseases and disorders. Students, researchers, professors, and clinicians should find the book's combination of "classic" and innovative techniques essential to the study of cilia and flagella.

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