Molecular genetics of mammalian cells. Volume 151 / edited by Michael M. Gottesman.

Contributor(s): Gottesman, Michael M [editor.]
Material type: TextTextSeries: Methods in enzymology: volume 151.Publisher: San Diego : Academic Press, ©1987Description: 1 online resource (xxx, 609 pages, 4 unnumbered pages of plates) : illustrations, portraitContent type: text Media type: computer Carrier type: online resourceSubject(s): Mammals -- Genetics | Molecular genetics | Cytogenetics | Cells | Mammals | Molecular Biology | Génétique moléculaire | Génétique du développement | Cytogenetics | Mammals -- Genetics | Molecular genetics | Säugetiere | Molekulargenetik | Aufsatzsammlung | Cytogenetik | Genen | Zoogdieren | Karakterisering (algemeen) | ZelleGenre/Form: Electronic books. | Electronic books. Additional physical formats: Print version:: Molecular genetics of mammalian cells.DDC classification: 574.1925 LOC classification: QP601 | .M49 vol. 151QH430Online resources: Click here to access online
Contents:
Chinese hamster ovary cells / Michael M. Gottesman -- Cultured S49 mouse T lymphoma cells / Theodoor van Daalen Wetters, Philip Coffino -- Liver cell lines / G. J. Darlington -- HeLa cell lines / Shin-ichi Akiyama -- Somatic cell genetic analysis of myelomas and hybridomas / Deborah French, Theresa Kelly, Susan Buhl, Matthew D. Scharff -- Mouse teratocarcinoma cells / Hedwig Jakob, Jean-François Nicolas -- Amplification of genes in somatic mammalian cells / Robert T. Schimke, David S. Roos, Peter C. Brown -- Replica plating and detection of antigen variants / Susannah Gal -- Drug-resistant mutants: Selection and dominance analysis / Michael M. Gottesman -- Suicide selection of mammalian cell mutants / David Patterson, Charles A. Waldren -- Use of tritium-labeled precursors to select mutants / Jacques Pouysségur, Arlette Franchi -- Construction of immunotoxins using Pseudomonas exotoxin A / David J. P. Fitzgerald -- Isolation of temperature-sensitive mutants / Joseph Hirschberg, Menashe Marcus -- Fluorescence-activated cell sorting of hybrid and transfected cells / Michael E. Kamarck -- Isozyme identification of chromosomes in interspecific somatic cell hybrids / Michael J. Siciliano, Billie F. White -- Principles of electrofusion and electropermeabilization / Ulrich Zimmermann, Howard B. Urnovitz -- Cell enucleation, cybrids, reconstituted cells, and nuclear hybrids / Jerry W. Shay -- Toxin -- antitoxin selection for isolating heterokaryons and cell hybrids / Woodring E. Wright -- Chromosome sorting by flow cytometry / Marty Bartholdi, Julie Meyne, Kevin Albright, Mary Luedemann, Evelyn Campbell, Douglas Chritton, Larry L. Deaven, L. Scott Cram -- Methods for chromosome banding of human and experimental tumors in Vitro / Jeffrey M. Trent, Floyd H. Thompson -- In situ hybridization of metaphase and prometaphase chromosomes / Susan L. Naylor, John R. McGill, Bernhard U. Zabel -- Gene mapping with sorted chromosomes / Roger V. Lebo, Barry D. Bruce -- Transfer and selective retention of single specific human chromosomes via microcell-mediated chromosome transfer / Paul J. Saxon, Eric J. Stanbridge -- Strategies for isolation of mutant genes / Michael M. Gottesman -- Use of in-gel DNA renaturation for detection and cloning of amplified genes / Igor B. Roninson -- Detection and characterization of specific mRNA by microinjection and complementation of mutant cells / Pin-Fang Lin, David B. Brown, Pat Murphy, Masaru Yamaizumi, Frank H. Ruddle -- Use of the CAT reporter gene for optimization of gene transfer into eukaryotic cells / C. Michael Fordis, Bruce H. Howard -- Cosmid vector systems for genomic DNA cloning / Mary McCormick, Max E. Gottesman, George A. Gaitanaris, Bruce H. Howard -- Preparation of a genomic cosmid library / Robert Fleischmann, Mary McCormick, Bruce H. Howard -- Colony screening of genomic cosmid libraries / Bruce R. Troen -- Rescue of genes transferred from cosmid libraries / Yun-Fai Lau -- Phage-mediated transduction of cDNA expression libraries into mammalian cells / Hiroto Okayama -- Sib selection / Mary McCormick -- Repetitive cloning of mutant genes using locus-specific sticky ends / Lawrence A. Chasin, Adelaide M. Carothers, Gail Urlaub -- Strategies for mapping and cloning macroregions of mammalian genomes / Cassandra L. Smith, Simon K. Lawrance, Gerald A. Gillespie, Charles R. Cantor, Sherman M. Weissman, Francis S. Collins -- Insertional mutagenesis to isolate genes / Stephen P. Goff -- Microcloning of mammalian metaphase chromosomes / Jan-Erik Edström, Rolf Kaiser, Dan Röhme -- Antisense RNA inhibition of endogenous genes / J. Timothy Stout, C. Thomas Caskey -- In situ detection of transcription in transfected cells using biotin-labeled molecular probes / Gilbert H. Smith -- RNA detection and localization in cells and tissue sections by in Situ hybridization of 35S-labeled RNA probes / Mary E. Harper, Lisa M. Marselle -- Electrophoretic assay for DNA-binding proteins / Alexander Varshavsky.
Summary: Strategies needed to isolate mutant cell lines and to use the mutants to clone and map the genes are presented in detail. This is the first book in which such methodology and molecular biology are combined. The preferred cell lines for genetic analysis are presented in the first section, followed by special techniques for isolating a wide range of mutants. The remainder of the book is devoted to genetic mapping and cloning of mutant genes. The final section presents special techniques in gene regulation.
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Includes bibliographical references and indexes.

Chinese hamster ovary cells / Michael M. Gottesman -- Cultured S49 mouse T lymphoma cells / Theodoor van Daalen Wetters, Philip Coffino -- Liver cell lines / G. J. Darlington -- HeLa cell lines / Shin-ichi Akiyama -- Somatic cell genetic analysis of myelomas and hybridomas / Deborah French, Theresa Kelly, Susan Buhl, Matthew D. Scharff -- Mouse teratocarcinoma cells / Hedwig Jakob, Jean-François Nicolas -- Amplification of genes in somatic mammalian cells / Robert T. Schimke, David S. Roos, Peter C. Brown -- Replica plating and detection of antigen variants / Susannah Gal -- Drug-resistant mutants: Selection and dominance analysis / Michael M. Gottesman -- Suicide selection of mammalian cell mutants / David Patterson, Charles A. Waldren -- Use of tritium-labeled precursors to select mutants / Jacques Pouysségur, Arlette Franchi -- Construction of immunotoxins using Pseudomonas exotoxin A / David J. P. Fitzgerald -- Isolation of temperature-sensitive mutants / Joseph Hirschberg, Menashe Marcus -- Fluorescence-activated cell sorting of hybrid and transfected cells / Michael E. Kamarck -- Isozyme identification of chromosomes in interspecific somatic cell hybrids / Michael J. Siciliano, Billie F. White -- Principles of electrofusion and electropermeabilization / Ulrich Zimmermann, Howard B. Urnovitz -- Cell enucleation, cybrids, reconstituted cells, and nuclear hybrids / Jerry W. Shay -- Toxin -- antitoxin selection for isolating heterokaryons and cell hybrids / Woodring E. Wright -- Chromosome sorting by flow cytometry / Marty Bartholdi, Julie Meyne, Kevin Albright, Mary Luedemann, Evelyn Campbell, Douglas Chritton, Larry L. Deaven, L. Scott Cram -- Methods for chromosome banding of human and experimental tumors in Vitro / Jeffrey M. Trent, Floyd H. Thompson -- In situ hybridization of metaphase and prometaphase chromosomes / Susan L. Naylor, John R. McGill, Bernhard U. Zabel -- Gene mapping with sorted chromosomes / Roger V. Lebo, Barry D. Bruce -- Transfer and selective retention of single specific human chromosomes via microcell-mediated chromosome transfer / Paul J. Saxon, Eric J. Stanbridge -- Strategies for isolation of mutant genes / Michael M. Gottesman -- Use of in-gel DNA renaturation for detection and cloning of amplified genes / Igor B. Roninson -- Detection and characterization of specific mRNA by microinjection and complementation of mutant cells / Pin-Fang Lin, David B. Brown, Pat Murphy, Masaru Yamaizumi, Frank H. Ruddle -- Use of the CAT reporter gene for optimization of gene transfer into eukaryotic cells / C. Michael Fordis, Bruce H. Howard -- Cosmid vector systems for genomic DNA cloning / Mary McCormick, Max E. Gottesman, George A. Gaitanaris, Bruce H. Howard -- Preparation of a genomic cosmid library / Robert Fleischmann, Mary McCormick, Bruce H. Howard -- Colony screening of genomic cosmid libraries / Bruce R. Troen -- Rescue of genes transferred from cosmid libraries / Yun-Fai Lau -- Phage-mediated transduction of cDNA expression libraries into mammalian cells / Hiroto Okayama -- Sib selection / Mary McCormick -- Repetitive cloning of mutant genes using locus-specific sticky ends / Lawrence A. Chasin, Adelaide M. Carothers, Gail Urlaub -- Strategies for mapping and cloning macroregions of mammalian genomes / Cassandra L. Smith, Simon K. Lawrance, Gerald A. Gillespie, Charles R. Cantor, Sherman M. Weissman, Francis S. Collins -- Insertional mutagenesis to isolate genes / Stephen P. Goff -- Microcloning of mammalian metaphase chromosomes / Jan-Erik Edström, Rolf Kaiser, Dan Röhme -- Antisense RNA inhibition of endogenous genes / J. Timothy Stout, C. Thomas Caskey -- In situ detection of transcription in transfected cells using biotin-labeled molecular probes / Gilbert H. Smith -- RNA detection and localization in cells and tissue sections by in Situ hybridization of 35S-labeled RNA probes / Mary E. Harper, Lisa M. Marselle -- Electrophoretic assay for DNA-binding proteins / Alexander Varshavsky.

Print version record.

Strategies needed to isolate mutant cell lines and to use the mutants to clone and map the genes are presented in detail. This is the first book in which such methodology and molecular biology are combined. The preferred cell lines for genetic analysis are presented in the first section, followed by special techniques for isolating a wide range of mutants. The remainder of the book is devoted to genetic mapping and cloning of mutant genes. The final section presents special techniques in gene regulation.

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