Guide to techniques in glycobiology / edited by William J. Lennarz, Gerald W. Hart.

Contributor(s): Lennarz, William J [editor.] | Hart, Gerald W [editor.]
Material type: TextTextSeries: Methods in enzymology: v. 230.Publisher: San Diego : Academic Press, ©1994Description: 1 online resource (xxvi, 567 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 0121821315; 9780121821319Subject(s): Glycomics -- Technique | Glycoconjugates -- Technique | Glycoproteins -- Technique | Glycoconjugates -- analysis | Oligosaccharides -- analysis | Glycoprotéines | Glycoconjugués | Glykokonjugate | Methode | Glykolipide | Glykoproteine | Aufsatzsammlung | Oligosaccharide | Chemische Analyse | Biochimie -- Technique | Glycoconjugués | Glycoprotéines | Glycosidases | Oligosaccharides -- AnalyseGenre/Form: Electronic books. Additional physical formats: Print version:: Guide to techniques in glycobiology.DDC classification: 574.19245 LOC classification: QP601 | .M49 v.230Other classification: WC 4355 | WD 5050 | CHE 850f | CHE 820f | CHE 840f Online resources: Click here to access online
Contents:
Carbohydrate composition analysis of glycoconjugates by gas-liquid chromatography/mass spectrometry / Roberta K. Merkle and Izabella Poppe -- Metabolic radiolabeling of glycoconjugates / Ajit Varki -- Nonmetabolic radiolabeling and tagging of glycoconjugates / Carl G. Gahmberg and Martti Tovanen -- Enzymatic deglycosylation of aspargine-linked glycans: purification, properties, and specificity of oligosaccharide-cleaving enzymes from Flavobacterium meningosepticum / Anthony L. Tarentino and Thomas H. Plummer, Jr. -- Release of oligosaccharides from glycoproteins by hydrazinolysis / T.P. Patel and R.B. Parekh -- Use of lectins in analysis of glycoconjugates / Richard D. Cummings -- Saccharide linkage analysis using methylation and other techniques / Rudolf Geyer and Hildegard Geyer.
Mass spectrometry of carbohydrate-containing bipolymers / Anne Dell [and others] -- ¹H nuclear magnetic resonance spectroscopy of carbohydrate chains of glycoproteins / Herman Van Halbeek -- Determination of sialic acids / Gerd Reuter and Roland Schauer -- Size fractionation of oligosaccharides / Akira Kobata -- High-pH anion-exchange chromatography of glycoprotein-derived carbohydrates / Mark R. Hardy and R. Reid Townsend -- High-performance liquid chromatography of pyridylaminated saccharides / Sumihiro Hase -- High-performance liquid chromatography of oligosaccharides / Jacques U. Baenziger -- High-resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides / Peter Jackson.
Capillary electrophoresis of oligosaccharides / Robert J. Linhardt -- Glycosidases in structural analysis / Gary S. Jacob and Peter Scudder -- Glycosyltransferases in glycobiology / Monica M. Palcic -- Glycosidase inhibitors in study of glycoconjugates / Gur P. Kaushal and Alan D. Elbein -- Synthesis and uses of azido-substituted nucleoside diphosphate sugar photoaffinity analogs / Anna Radominska and Richard R. Drake -- Glycoform analysis of glycoproteins / R.B. Parekh -- Isolation of glycosphingolipids / Ronald L. Schnaar -- Thin-layer chromatography of glycosphingolipids / Ronald L. Schnaar and Leila K. Needham -- Isolation and characterization of proteoglycans / Vincent C. Hascall [and others] -- Structural analysis of glycosylphosphatidylinositol anchors / Anant K. Menon.
Detection of O-linked N-acetylglucosamine (O-GlcNAc) on cytoplasmic and nuclear proteins / Elizabeth P. Roquemore, Teh-Ying Chou and Gerald W. Hart -- Identification of polysialic acids in glycoconjugates / Jean Ye [and others] -- Neoglycolipids: probes of oligosaccharide structure, antigenicity, and function / Ten Feizi [and others].
Action note: digitized 2011 committed to preserveSummary: The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for carbohydrate chain analysis Among the topics covered: * Release of Oligosaccharides from Glycoproteins by Hydrazinolysis * Mass Spectrometry of Carbohydrate-Containing Biopolymers * High-resolution Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Reducing Saccharides * Capillary Electrophoresis of Oligosaccharides * Synthesis and Uses of Azido-Substituted Nucleoside Diphosphate Sugar Photoaffinity Analogs * Structural Analysis of Glycosylphosphatidylinositol Anchors * Identification of Polysialic Acids in Glycoconjugates.
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Includes bibliographical references and indexes.

Carbohydrate composition analysis of glycoconjugates by gas-liquid chromatography/mass spectrometry / Roberta K. Merkle and Izabella Poppe -- Metabolic radiolabeling of glycoconjugates / Ajit Varki -- Nonmetabolic radiolabeling and tagging of glycoconjugates / Carl G. Gahmberg and Martti Tovanen -- Enzymatic deglycosylation of aspargine-linked glycans: purification, properties, and specificity of oligosaccharide-cleaving enzymes from Flavobacterium meningosepticum / Anthony L. Tarentino and Thomas H. Plummer, Jr. -- Release of oligosaccharides from glycoproteins by hydrazinolysis / T.P. Patel and R.B. Parekh -- Use of lectins in analysis of glycoconjugates / Richard D. Cummings -- Saccharide linkage analysis using methylation and other techniques / Rudolf Geyer and Hildegard Geyer.

Mass spectrometry of carbohydrate-containing bipolymers / Anne Dell [and others] -- ¹H nuclear magnetic resonance spectroscopy of carbohydrate chains of glycoproteins / Herman Van Halbeek -- Determination of sialic acids / Gerd Reuter and Roland Schauer -- Size fractionation of oligosaccharides / Akira Kobata -- High-pH anion-exchange chromatography of glycoprotein-derived carbohydrates / Mark R. Hardy and R. Reid Townsend -- High-performance liquid chromatography of pyridylaminated saccharides / Sumihiro Hase -- High-performance liquid chromatography of oligosaccharides / Jacques U. Baenziger -- High-resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides / Peter Jackson.

Capillary electrophoresis of oligosaccharides / Robert J. Linhardt -- Glycosidases in structural analysis / Gary S. Jacob and Peter Scudder -- Glycosyltransferases in glycobiology / Monica M. Palcic -- Glycosidase inhibitors in study of glycoconjugates / Gur P. Kaushal and Alan D. Elbein -- Synthesis and uses of azido-substituted nucleoside diphosphate sugar photoaffinity analogs / Anna Radominska and Richard R. Drake -- Glycoform analysis of glycoproteins / R.B. Parekh -- Isolation of glycosphingolipids / Ronald L. Schnaar -- Thin-layer chromatography of glycosphingolipids / Ronald L. Schnaar and Leila K. Needham -- Isolation and characterization of proteoglycans / Vincent C. Hascall [and others] -- Structural analysis of glycosylphosphatidylinositol anchors / Anant K. Menon.

Detection of O-linked N-acetylglucosamine (O-GlcNAc) on cytoplasmic and nuclear proteins / Elizabeth P. Roquemore, Teh-Ying Chou and Gerald W. Hart -- Identification of polysialic acids in glycoconjugates / Jean Ye [and others] -- Neoglycolipids: probes of oligosaccharide structure, antigenicity, and function / Ten Feizi [and others].

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for carbohydrate chain analysis Among the topics covered: * Release of Oligosaccharides from Glycoproteins by Hydrazinolysis * Mass Spectrometry of Carbohydrate-Containing Biopolymers * High-resolution Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Reducing Saccharides * Capillary Electrophoresis of Oligosaccharides * Synthesis and Uses of Azido-Substituted Nucleoside Diphosphate Sugar Photoaffinity Analogs * Structural Analysis of Glycosylphosphatidylinositol Anchors * Identification of Polysialic Acids in Glycoconjugates.

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