DNA replication / edited by Judith L. Campbell.

Contributor(s): Campbell, Judith L [editor.]
Material type: TextTextSeries: Methods in enzymology: v. 262.Publisher: San Diego : Academic Press, ©1995Description: 1 online resource (xxix, 716 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceSubject(s): DNA replication | ADN -- Réplication | DNA replication | DNA | Replicatie (biochemie) | Polymerase kettingreactie | Enzymologia -- podręczniki laboratoryjne | DNA -- replikacja | ADN -- Synthèse | ADN polymérases | ADN -- Réplication | Replikation | Enzymologie | Methode | Aufsatzsammlung | DNA Replication -- physiology | DNA-Directed DNA Polymerase -- physiology | DNA SynthesisGenre/Form: Electronic books. Additional physical formats: Print version:: DNA replication.DDC classification: 574.873282 LOC classification: QP601 | .M49 vol. 262Other classification: 42.20 | 35.71 | 35.73 Online resources: Click here to access online
Contents:
Purification and characterization of DNA polymerases --Structural analysis of DNA polymerases -- Mechanisms of DNA polymerases -- Structure-function studies of DNA polymerases -- Polymerase accessory functions, replication proteins, multienzyme replication complexes -- In vitro replication systems: crude and reconstituted -- DNA synthesis in vivo.
Summary: The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Includes descriptions of functional, structural, kinetic, and genetic methods for analyzing major enzymes of DNA replication * Describes strategies for studying interactions of these proteins during replication * Provides comprehensive descriptions of uses of prokaryotic and eukaryotic crude in vitro replication systems and reconstitution of such systems from purified proteins * Includes methods for analyzing DNA replication in vivo.
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Includes bibliographical references and indexes.

Purification and characterization of DNA polymerases --Structural analysis of DNA polymerases -- Mechanisms of DNA polymerases -- Structure-function studies of DNA polymerases -- Polymerase accessory functions, replication proteins, multienzyme replication complexes -- In vitro replication systems: crude and reconstituted -- DNA synthesis in vivo.

The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Includes descriptions of functional, structural, kinetic, and genetic methods for analyzing major enzymes of DNA replication * Describes strategies for studying interactions of these proteins during replication * Provides comprehensive descriptions of uses of prokaryotic and eukaryotic crude in vitro replication systems and reconstitution of such systems from purified proteins * Includes methods for analyzing DNA replication in vivo.

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