Membrane transport : a practical approach / edited by Stephen A. Baldwin.

Contributor(s): Baldwin, Stephen A
Material type: TextTextSeries: Practical approach series: 230.Publisher: Oxford ; New York : Oxford University Press, 2000Description: 1 online resource (xxi, 320 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9780191565823; 0191565822Subject(s): Carrier proteins -- Laboratory manuals | Biological transport -- Laboratory manuals | Cell membranes -- Experiments | Carrier proteins -- Experiments | Carrier Proteins -- physiology -- Laboratory Manuals | Cell Membrane -- physiology -- Laboratory Manuals | Cells -- metabolism -- Laboratory Manuals | Cytological Techniques -- Laboratory Manuals | Protein Transport -- physiology -- Laboratory Manuals | SCIENCE -- Life Sciences -- Biochemistry | Biological transport | Carrier proteins | Membrantransport | Membranen | Transportverschijnselen | Biomoleculen | Eiwitten | Membranas celulares | Transporte através da membranaGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Membrane transport.DDC classification: 572/.69 LOC classification: QP552.C34 | M46 2000ebOther classification: 58.13 | 42.15 | BIO 200f | CHE 887f | WE 5400 Online resources: Click here to access online
Contents:
Assay of membrane transport in cells and membrane vesicles / Simon M. Jarvis -- Reconstitution of membrane proteins : the Ca²-ATPase of sarcoplasmic reticulum / Anthony G. Lee -- The Xenopus oocyte expression system for the cDNA cloning and characterization of plasma membrane transport proteins / Sylvia Y.M. Yao, Carol E. Cass, and James D. Young -- Expression of foreign transport proteins in yeast / N. Sauer and J. Stolz -- Baculovirus-mediated overexpression of transport proteins / Gary J. Litherland and Stephen A. Baldwin -- The amplified expression, identification, purification, assay, and properties of hexahistidine-tagged bacterial membrane transport proteins / Alison Ward [and others] -- Spectroscopic and kinetic approaches for probing the mechanisms of solute transporters / Adrian R. Walmsley -- Detection and analysis of glucose transporters using photolabelling techniques / Alison K. Gillingham [and others] -- Computer prediction of transporter topology and structure / Rong-I Hong and Mark S.P. Sansom -- Two-dimensional crystallization of membrane proteins / Philippe Ringler, Bernard Heymann, and Andreas Engel -- Crystallization of membrane proteins / Tina D. Howard, Katherine E. McAuley-Hecht, and Richard J. Cogdell.
Summary: Membrane Transport contains a collection of experimental protocols for the study of proteins responsible for the transport of small molecules across biological membranes. It includes information on the latest methods for production of transport proteins by recombinant DNA technology, and for their investigation by techniques accessible to any well-found laboratory.
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Includes bibliographical references and index.

Assay of membrane transport in cells and membrane vesicles / Simon M. Jarvis -- Reconstitution of membrane proteins : the Ca²-ATPase of sarcoplasmic reticulum / Anthony G. Lee -- The Xenopus oocyte expression system for the cDNA cloning and characterization of plasma membrane transport proteins / Sylvia Y.M. Yao, Carol E. Cass, and James D. Young -- Expression of foreign transport proteins in yeast / N. Sauer and J. Stolz -- Baculovirus-mediated overexpression of transport proteins / Gary J. Litherland and Stephen A. Baldwin -- The amplified expression, identification, purification, assay, and properties of hexahistidine-tagged bacterial membrane transport proteins / Alison Ward [and others] -- Spectroscopic and kinetic approaches for probing the mechanisms of solute transporters / Adrian R. Walmsley -- Detection and analysis of glucose transporters using photolabelling techniques / Alison K. Gillingham [and others] -- Computer prediction of transporter topology and structure / Rong-I Hong and Mark S.P. Sansom -- Two-dimensional crystallization of membrane proteins / Philippe Ringler, Bernard Heymann, and Andreas Engel -- Crystallization of membrane proteins / Tina D. Howard, Katherine E. McAuley-Hecht, and Richard J. Cogdell.

Print version record.

Membrane Transport contains a collection of experimental protocols for the study of proteins responsible for the transport of small molecules across biological membranes. It includes information on the latest methods for production of transport proteins by recombinant DNA technology, and for their investigation by techniques accessible to any well-found laboratory.

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