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A practical manual for musculoskeletal research / editors Kwok-Sui Leung [and others] ; associate editors Kwong-Man Lee, Jian-Quan Feng, Chun-Wai Chan.

Contributor(s): Leung, Kwok-suiMaterial type: TextTextPublication details: Singapore ; Hackensack, NJ : World Scientific, ©2008. Description: 1 online resource (xliii, 896 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9789812794093; 9812794093Subject(s): Musculoskeletal system | MEDICAL -- Anatomy | SCIENCE -- Life Sciences -- Human Anatomy & Physiology | Musculoskeletal system | Musculoskeletal Physiological Phenomena | Musculoskeletal System -- anatomy & histology | Biomedical Engineering -- methods | Biophysics -- methods | Models, Biological | Molecular Biology -- methodsGenre/Form: Electronic books. | Electronic books. Additional physical formats: Print version:: Practical manual for musculoskeletal research.DDC classification: 611.7 LOC classification: QM100 | .P725 2008ebNLM classification: 2008 M-780 | WE 101Online resources: Click here to access online
Contents:
Pt. I. Cell culture and molecular biology. ch. 1. DNA microarray / Xiao-Ling Zhang and Yan-Zhi Du. ch. 2. Visualizing gene products: immunohistochemistry, in situ hybridization, and staining for [symbol]-galactosidase activity / Yong-Bo Lu, Yi-Xia Xie and Jian-Quan Feng. ch. 3. Mesenchymal stem cell culture, expansion, and osteogenic and adipogenic differentiation / Hui Sheng ... [et al.]. ch. 4. Stem cells and their role in bone formation and regeneration / Yi-Zhi Meng and Yi-Xian Qin. ch. 5. Stem cells and tissue engineering applications in the musculoskeletal system / Chang-Hun Lee ... [et al.]. ch. 6. Osteoblast culture and pharmacological evaluation in vitro / Hong-Fu Wang ... [et al.]. ch. 7. Osteoclast culture and pharmacological evaluation in vitro / Jian-Jun Cao ... [et al.]. ch. 8. Primary cultures of human periosteal cells / Wing-Hoi Cheung ... [et al.]. ch. 9. Visualization of osteocytes and mineralization / Yi-Xia Xie ... [et al.]. ch. 10. Tissue culture of giant cell tumor of bone / Lin Huang and Ming-Hao Zheng. ch. 11. Chondrocyte mechanotransduction in three-dimensional cell culture / Xu Yang, Riaz Gillani and Qian Chen. ch. 12. Chondrocyte-pellet culture for cartilage repair research / Wing-Hoi Cheung ... [et al.] -- pt. II. Histology and histomorphometry. ch. 13. Tissue preparations / Yong-Bo Lu, Yi-Xia Xie and Jian-Quan Feng. ch. 14. Acceleration of bone decalcification by ultrasound / Xia Guo and Wai-Ling Lam. ch. 15. Stains of bone and cartilage / Yong-Bo Lu, Yi-Xia Xie and Jian-Quan Feng. ch. 16. Contact microradiography for studying the degree of bone mineralization / Yong-Ping Cao ... [et al.]. ch. 17. Undecalcified histology in studying hard tissue implanted with calcium phosphate-based ceramics / Chun-Wai Chan and Ling Qin -- pt. III. ch. 18. Protocols of micro-computed tomographic analysis established for musculoskeletal applications / Hiu-Yan Yeung ... [et al.]. ch. 19. Microangiography for studying neovascularization during long bone fracture repair in a rat model / Xiao-Zhong Zhou ... [et al]. ch. 20. High-resolution imaging of organs and tissues by in vivo micro-computed tomography / Engin Ozcivici ... [et al.]. ch. 21. Positron emission tomography of bone in small animals / Erik Mittra, Shahriar S. Yaghoubi and Yi-Xian Qin -- pt. IV. Laboratory animal models. ch. 22. Surgical anesthesia and analgesia for animals in musculoskeletal research / Dewi K. Rowlands and Anthony E. James. ch. 23. Mouse model of calvarial osteolysis / Chao Zhang and Ting-Ting Tang. ch. 24. Distraction osteogenesis model / Chun-Wai Chan and Kwok-Sui Leung. ch. 25. Fracture nonunion animal model / Xia Guo ... [et al.]. ch. 26. Establishment of osteoporosis model in goats / Wing-Sum Siu, Ling Qin and Kwok-Sui Leung. ch. 27. Posterior spinal fusion model / Chun-Wai Chan and Jack Chun-Yiu Cheng. ch. 28. Functional disuse model for musculoskeletal adaptation / Ho-Yan Lam and Yi-Xian Qin. ch. 29. Neurogenic limb disuse animal models / Xia Guo, Xiao-Yun Wang and Wai-Ling Lam. ch. 30. Establishment of steroid-associated osteonecrosis rabbit model / Ge Zhang, Ling Qin and Hui Sheng. ch. 31. Establishment of anterior cruciate ligament reconstruction model in rabbit / Chun-Yi Wen and Kai-Ming Chan. ch. 32. Establishment of normal and delayed bone-tendon junction repair models / Kwok-Sui Leung and Ling Qin. ch. 33. Anterior cruciate ligament transection (ACLT)-induced osteoarthritis in rats / Ya-Feng Zhang, Jun-Fei Wang and Ge Zhang. ch. 34. Establishment of rabbit partial growth plate defect model / Kwoon-Ho Chow ... [et al.] -- pt. V. X-ray- and MRI-based densitometry. ch. 35. Micro-CT 3D image analysis techniques for orthopedic applications: metal implant-to-bone contact surface and porosity of biomaterials / Phil Salmon. ch. 36. Application of DXA to assess orthopedic implants / Tom V. Sanchez and Jing-Mei Wang. ch. 37. Clinical monitoring of bone mineralization in distraction osteogenesis using DXA / Vivian Wing-Yin Hung, Bobby Kin-Wah Ng and Jack Chun-Yiu Cheng. ch. 38. In vivo and ex vivo bone mineral density and structure measurements using XtremeCT[symbol] -- a high-resolution pQCT (HRpQCT) / Maurus Neff ... [et al.]. ch. 39. Advanced 3D image processing methods for quantifying proximal femur and vertebra structures from QCT images / Wen-Jun Li, Ying Lu and Thomas Lang. ch. 40. Micro-finite element analysis of b-one / He Gong, Ming Zhang and Ling Qin. ch. 41. The characterization of cortical bone water distribution and structure changes on age, microdamage, and disuse by nuclear magnetic resonance / Qing-Wen Ni ... [et al.]. ch. 42. Dynamic contrast-enhanced magnetic resonance imaging of the musculoskeletal system: basic principles and clinical applications in bone sarcomas and pheumatoid arthritis / Yi-Xiang Wang. ch. 43. Noninvasive evaluation of knee cartilage morphology by magnetic resonance imaging / Yi-Xiang Wang -- pt. VI. Biomechanics and motion analysis. ch. 44. Cell traction force microscopy for musculoskeletal research / James Hui-Cong Wang, Bin Li and Jeen-Shang Lin. ch. 45. Nanoindentation: techniques and technical considerations for musculoskeletal research / Suzanne Ferreri, Stefan Judex and Yi-Xian Qin. ch. 46. Micromechanical testing of bone tissues in tension / Xiao-Du Wang ... [et al.]. ch. 47. Technical manual for biomechanical testing of musculoskeletal tissues / Daniel Hung-Kay Chow ... [et al.]. ch. 48. Motion analysis in musculoskeletal research / Zong-Ming Li.
Summary: This manual provides technical protocols for musculoskeletal research on a translational basis, i.e. a disease-orientated approach. It offers guidance on various laboratory techniques, including cell culture and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal models, CT- and MRI-based densitometry and microarchitectural analysis, biomechanics and functional analysis of orthopedic kinesiology, etc. The content is simple and straightforward, with illustrations and step-by-step procedures as an easy experimental reference for personnel in basic and clinical musculoskeletal research and education. This book will provide a unique multidisciplinary platform for various professions - not only orthopedics, but also biomedical engineering and biomaterial sciences - involving both basic and clinical medicine.
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Includes bibliographical references and index.

Print version record.

Pt. I. Cell culture and molecular biology. ch. 1. DNA microarray / Xiao-Ling Zhang and Yan-Zhi Du. ch. 2. Visualizing gene products: immunohistochemistry, in situ hybridization, and staining for [symbol]-galactosidase activity / Yong-Bo Lu, Yi-Xia Xie and Jian-Quan Feng. ch. 3. Mesenchymal stem cell culture, expansion, and osteogenic and adipogenic differentiation / Hui Sheng ... [et al.]. ch. 4. Stem cells and their role in bone formation and regeneration / Yi-Zhi Meng and Yi-Xian Qin. ch. 5. Stem cells and tissue engineering applications in the musculoskeletal system / Chang-Hun Lee ... [et al.]. ch. 6. Osteoblast culture and pharmacological evaluation in vitro / Hong-Fu Wang ... [et al.]. ch. 7. Osteoclast culture and pharmacological evaluation in vitro / Jian-Jun Cao ... [et al.]. ch. 8. Primary cultures of human periosteal cells / Wing-Hoi Cheung ... [et al.]. ch. 9. Visualization of osteocytes and mineralization / Yi-Xia Xie ... [et al.]. ch. 10. Tissue culture of giant cell tumor of bone / Lin Huang and Ming-Hao Zheng. ch. 11. Chondrocyte mechanotransduction in three-dimensional cell culture / Xu Yang, Riaz Gillani and Qian Chen. ch. 12. Chondrocyte-pellet culture for cartilage repair research / Wing-Hoi Cheung ... [et al.] -- pt. II. Histology and histomorphometry. ch. 13. Tissue preparations / Yong-Bo Lu, Yi-Xia Xie and Jian-Quan Feng. ch. 14. Acceleration of bone decalcification by ultrasound / Xia Guo and Wai-Ling Lam. ch. 15. Stains of bone and cartilage / Yong-Bo Lu, Yi-Xia Xie and Jian-Quan Feng. ch. 16. Contact microradiography for studying the degree of bone mineralization / Yong-Ping Cao ... [et al.]. ch. 17. Undecalcified histology in studying hard tissue implanted with calcium phosphate-based ceramics / Chun-Wai Chan and Ling Qin -- pt. III. ch. 18. Protocols of micro-computed tomographic analysis established for musculoskeletal applications / Hiu-Yan Yeung ... [et al.]. ch. 19. Microangiography for studying neovascularization during long bone fracture repair in a rat model / Xiao-Zhong Zhou ... [et al]. ch. 20. High-resolution imaging of organs and tissues by in vivo micro-computed tomography / Engin Ozcivici ... [et al.]. ch. 21. Positron emission tomography of bone in small animals / Erik Mittra, Shahriar S. Yaghoubi and Yi-Xian Qin -- pt. IV. Laboratory animal models. ch. 22. Surgical anesthesia and analgesia for animals in musculoskeletal research / Dewi K. Rowlands and Anthony E. James. ch. 23. Mouse model of calvarial osteolysis / Chao Zhang and Ting-Ting Tang. ch. 24. Distraction osteogenesis model / Chun-Wai Chan and Kwok-Sui Leung. ch. 25. Fracture nonunion animal model / Xia Guo ... [et al.]. ch. 26. Establishment of osteoporosis model in goats / Wing-Sum Siu, Ling Qin and Kwok-Sui Leung. ch. 27. Posterior spinal fusion model / Chun-Wai Chan and Jack Chun-Yiu Cheng. ch. 28. Functional disuse model for musculoskeletal adaptation / Ho-Yan Lam and Yi-Xian Qin. ch. 29. Neurogenic limb disuse animal models / Xia Guo, Xiao-Yun Wang and Wai-Ling Lam. ch. 30. Establishment of steroid-associated osteonecrosis rabbit model / Ge Zhang, Ling Qin and Hui Sheng. ch. 31. Establishment of anterior cruciate ligament reconstruction model in rabbit / Chun-Yi Wen and Kai-Ming Chan. ch. 32. Establishment of normal and delayed bone-tendon junction repair models / Kwok-Sui Leung and Ling Qin. ch. 33. Anterior cruciate ligament transection (ACLT)-induced osteoarthritis in rats / Ya-Feng Zhang, Jun-Fei Wang and Ge Zhang. ch. 34. Establishment of rabbit partial growth plate defect model / Kwoon-Ho Chow ... [et al.] -- pt. V. X-ray- and MRI-based densitometry. ch. 35. Micro-CT 3D image analysis techniques for orthopedic applications: metal implant-to-bone contact surface and porosity of biomaterials / Phil Salmon. ch. 36. Application of DXA to assess orthopedic implants / Tom V. Sanchez and Jing-Mei Wang. ch. 37. Clinical monitoring of bone mineralization in distraction osteogenesis using DXA / Vivian Wing-Yin Hung, Bobby Kin-Wah Ng and Jack Chun-Yiu Cheng. ch. 38. In vivo and ex vivo bone mineral density and structure measurements using XtremeCT[symbol] -- a high-resolution pQCT (HRpQCT) / Maurus Neff ... [et al.]. ch. 39. Advanced 3D image processing methods for quantifying proximal femur and vertebra structures from QCT images / Wen-Jun Li, Ying Lu and Thomas Lang. ch. 40. Micro-finite element analysis of b-one / He Gong, Ming Zhang and Ling Qin. ch. 41. The characterization of cortical bone water distribution and structure changes on age, microdamage, and disuse by nuclear magnetic resonance / Qing-Wen Ni ... [et al.]. ch. 42. Dynamic contrast-enhanced magnetic resonance imaging of the musculoskeletal system: basic principles and clinical applications in bone sarcomas and pheumatoid arthritis / Yi-Xiang Wang. ch. 43. Noninvasive evaluation of knee cartilage morphology by magnetic resonance imaging / Yi-Xiang Wang -- pt. VI. Biomechanics and motion analysis. ch. 44. Cell traction force microscopy for musculoskeletal research / James Hui-Cong Wang, Bin Li and Jeen-Shang Lin. ch. 45. Nanoindentation: techniques and technical considerations for musculoskeletal research / Suzanne Ferreri, Stefan Judex and Yi-Xian Qin. ch. 46. Micromechanical testing of bone tissues in tension / Xiao-Du Wang ... [et al.]. ch. 47. Technical manual for biomechanical testing of musculoskeletal tissues / Daniel Hung-Kay Chow ... [et al.]. ch. 48. Motion analysis in musculoskeletal research / Zong-Ming Li.

This manual provides technical protocols for musculoskeletal research on a translational basis, i.e. a disease-orientated approach. It offers guidance on various laboratory techniques, including cell culture and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal models, CT- and MRI-based densitometry and microarchitectural analysis, biomechanics and functional analysis of orthopedic kinesiology, etc. The content is simple and straightforward, with illustrations and step-by-step procedures as an easy experimental reference for personnel in basic and clinical musculoskeletal research and education. This book will provide a unique multidisciplinary platform for various professions - not only orthopedics, but also biomedical engineering and biomaterial sciences - involving both basic and clinical medicine.

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