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Platelets and megakaryocytes. Volume 1, Functional assays / edited by Jonathan M. Gibbins, Martyn P. Mahaut-Smith.

Contributor(s): Gibbins, Jonathan M | Mahaut-Smith, Martyn PMaterial type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 272.Publication details: Totowa, N.J. : Humana Press, ©2004. Description: 1 online resource (1 volume) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 1592597823; 9781592597826; 1588291014; 9781588291011Other title: Functional assaysSubject(s): Blood platelets -- Laboratory manuals | Megakaryocytes -- Laboratory manuals | Histology -- Technique | Blood Platelets -- physiology | Histological Techniques | Megakaryocytes -- physiology | Sang -- Plaquettes -- Manuels de laboratoire | Mégacaryocytes -- Manuels de laboratoire | Histologie -- Technique | MEDICAL -- Physiology | SCIENCE -- Life Sciences -- Human Anatomy & Physiology | Blood platelets | Megakaryocytes | bloedplaatjes | platelets | beenmergcellen | bone marrow cells | bloedvorming | haematopoiesis | bloedcellen | blood cells | bloedanalyse | blood analysis | moleculaire biologie | molecular biology | biologische technieken | biological techniques | protocollen | protocols | Histology | Molecular Biology (General) | Histologie | Moleculaire biologie (algemeen)Genre/Form: Electronic books. | Laboratory manuals.Additional physical formats: Print version:: Platelets and megakaryocytes. Volume 1, Functional assays.DDC classification: 612.1/17 LOC classification: QP97 | .P568 2004ebNLM classification: W1 | WH 300Online resources: Click here to access online
Contents:
v. 1. Functional assays -- v. 2. Perspectives and techniques.
Effects of anticoagulants used during blood collection on human platelet function / J.A. May and S. Heptinstall -- Preparation of washed platelet suspensions from human and rodent blood / J.P. Cazenave [and others] -- Platelet counting / P. Harrison, C. Briggs and S.J. Machin -- Electron microscopy methods for studying platelet structure and function / J.G. White -- Platelet aggregation: Turbidimetric measurements / G.E. Jarvis -- Platelet aggregation in whole blood: Impedance and particle counting methods / G.E. Jarvis -- Secretion from dense granules: Luminescence method for adenine nucleotides / M.F. Heath -- Platelet dense-granule secretion: The [3H]-5-HT secretion assay / D. Crosby and A.W. Poole -- Analysis of the releasable nucleotides of platelets / J. Jankowski, L. Henning and H. Schluter -- Studies of secretion using permeabilized platelets / T.W. Rutledge and S.W. Whiteheart -- Measurement of platelet arachidonic acid metabolism / R.W. Farndale [and others] -- Measurement of the platelet procoagulant response / J.W. Heemskerk [and others] -- Platelet adhesion assays performed under static conditions / J.M. Stevens -- Platelet adhesion assays under flow using matrix protein-coupled adhesion columns / R. Polanowska-Grabowska and A.R. Gear -- Techniques to examine platelet adhesive interactions under flow / S. Kulkarni [and others] -- In vivo models of platelet function and thrombosis: Study of real-time thrombus formation / S. Falati [and others] -- Adhesion between platelets and leukocytes or endothelial cells / G.B. Nash -- In vitro measurement of high-shear platelet adhesion and aggregation by the PFA-100 / P. Harrison -- Flow-cytometric analysis of platelet-membrane glycoprotein expression and platelet activation / A.H. Goodall and J. Appleby -- Flow-cytometric analysis of mouse platelet function / B. Nieswandt, V. Schulte and W. Bergmeier -- Platelet microparticles / S. Nomura and S. Fukuhara -- Isolation of primary megakaryocytes and studies of proplatelet formation / R.M. Leven -- Isolation and culture of megakaryocyte precursors / N. Debili, F. Louache and W. Vainchenker -- Assays of megakaryocyte development: Surface antigen expression, ploidy, and size / A. Mathur [and others] -- Assays of megakaryocyte development: Cytoplasm, storage granules, and demarcation membranes / A. Drouin, G. Alimardani and E.M. Cramer -- Thrombopoietin bioassay / W.S. Alexander and C. Hyland -- Culture of megakaryocytic cell lines: Uses and limitations / N. Komatsu.
Summary: Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 1: Functional Assays, leading expert researchers-many responsible for seminal discoveries in the field-describe basic and advanced techniques for analyzing platelet and megakaryocyte function in step-by-step detail. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. Techniques presented for megakaryocyte function research include those used to study the development of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques offers a state-of-the-art survey of our understanding of these cells, with emphasis on the role of cell signaling in their regulation and function, as well as the use of molecular and post-genomic techniques for their study. Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.
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Includes bibliographical references and index.

v. 1. Functional assays -- v. 2. Perspectives and techniques.

Effects of anticoagulants used during blood collection on human platelet function / J.A. May and S. Heptinstall -- Preparation of washed platelet suspensions from human and rodent blood / J.P. Cazenave [and others] -- Platelet counting / P. Harrison, C. Briggs and S.J. Machin -- Electron microscopy methods for studying platelet structure and function / J.G. White -- Platelet aggregation: Turbidimetric measurements / G.E. Jarvis -- Platelet aggregation in whole blood: Impedance and particle counting methods / G.E. Jarvis -- Secretion from dense granules: Luminescence method for adenine nucleotides / M.F. Heath -- Platelet dense-granule secretion: The [3H]-5-HT secretion assay / D. Crosby and A.W. Poole -- Analysis of the releasable nucleotides of platelets / J. Jankowski, L. Henning and H. Schluter -- Studies of secretion using permeabilized platelets / T.W. Rutledge and S.W. Whiteheart -- Measurement of platelet arachidonic acid metabolism / R.W. Farndale [and others] -- Measurement of the platelet procoagulant response / J.W. Heemskerk [and others] -- Platelet adhesion assays performed under static conditions / J.M. Stevens -- Platelet adhesion assays under flow using matrix protein-coupled adhesion columns / R. Polanowska-Grabowska and A.R. Gear -- Techniques to examine platelet adhesive interactions under flow / S. Kulkarni [and others] -- In vivo models of platelet function and thrombosis: Study of real-time thrombus formation / S. Falati [and others] -- Adhesion between platelets and leukocytes or endothelial cells / G.B. Nash -- In vitro measurement of high-shear platelet adhesion and aggregation by the PFA-100 / P. Harrison -- Flow-cytometric analysis of platelet-membrane glycoprotein expression and platelet activation / A.H. Goodall and J. Appleby -- Flow-cytometric analysis of mouse platelet function / B. Nieswandt, V. Schulte and W. Bergmeier -- Platelet microparticles / S. Nomura and S. Fukuhara -- Isolation of primary megakaryocytes and studies of proplatelet formation / R.M. Leven -- Isolation and culture of megakaryocyte precursors / N. Debili, F. Louache and W. Vainchenker -- Assays of megakaryocyte development: Surface antigen expression, ploidy, and size / A. Mathur [and others] -- Assays of megakaryocyte development: Cytoplasm, storage granules, and demarcation membranes / A. Drouin, G. Alimardani and E.M. Cramer -- Thrombopoietin bioassay / W.S. Alexander and C. Hyland -- Culture of megakaryocytic cell lines: Uses and limitations / N. Komatsu.

Print version record.

English.

Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 1: Functional Assays, leading expert researchers-many responsible for seminal discoveries in the field-describe basic and advanced techniques for analyzing platelet and megakaryocyte function in step-by-step detail. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. Techniques presented for megakaryocyte function research include those used to study the development of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques offers a state-of-the-art survey of our understanding of these cells, with emphasis on the role of cell signaling in their regulation and function, as well as the use of molecular and post-genomic techniques for their study. Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

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Platelets and megakaryocytes. ©2012
Platelets and megakaryocytes.

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