Chemotaxis : methods and protocols / edited by Tian Jin and Dale Hereld.

Includes bibliographical references and index.

Microfluidic techniques for the analysis of bacterial chemotaxis / D.L. Englert, A. Jayaraman and M.D. Manson -- Prokaryotic phototaxis / W.D. Hoff [and others] -- Photoorientation in photosynthetic flagellates / D.P. Hader and M. Lebert -- Dictyostelium slug phototaxis / S.J. Annesley and P.R. Fisher -- Electrotaxis and wound healing: experimental methods to study electric fields as a directional signal for cell migration / G. Tai [and others] -- Chemotropism during yeast mating / P.J. Follette and R.A. Arkowitz -- Group migration and signal relay in Dictyostelium / P.W. Kriebel and C.A. Parent -- Quantitative analysis of distal tip cell migration in C. elegans / M. Lee and E.J. Cram -- Inflammation and wound healing in Drosophila / B. Stramer and W. Wood -- Neutrophil motility in vivo using zebrafish / J.R. Mathias, K.B. Walters and A. Huttenlocher -- Chemotaxis in neutrophil-like HL-60 cells / A. Millius and O.D. Weiner -- Chemokine receptor dimerization and chemotaxis / J.M. Rodriguez-Frade [and others] -- Intravital two-photon imaging of adoptively transferred B lymphocytes in inguinal lymph nodes / C. Park, I.Y. Hwang and J.H. Kehrl -- Breast cancer cell movement: imaging invadopodia by TIRF and IRM microscopy / X. Xu, P. Johnson and S.C. Mueller -- In vivo assay for tumor cell invasion / L. Hernandez [and others] -- Quantitative studies of neuronal chemotaxis in 3D / W.J. Rosoff [and others] -- Assays for chemotaxis and chemoattractant-stimulated TorC2 activation and PKB substrate phosphorylation in Dictyostelium / Y. Kamimura, M. Tang and P. Devreotes -- Biochemical responses to chemoattractants in Dictyostelium: ligand-receptor interactions and downstream kinase activation / X.H. Liao and A.R. Kimmel -- Quantifying in vivo phosphoinositide turnover in chemotactically competent Dictyostelium cells / N. Pawolleck and R.S. Williams -- In vivo measurements of cytosolic calcium in Dictyostelium discoideum / C.Y. Allan and P.R. Fisher -- Chemokine receptor signaling and HIV infection / Y. Wu -- Spatiotemporal stimulation of single cells using flow photolysis / C. Beta -- Spatiotemporal regulation of Ras-GTPases during chemotaxis / A.T. Sasaki and R.A. Firtel -- FRAP analysis of chemosensory components of Dictyostelium / C.A. Elzie and C. Janetopoulos -- Monitoring dynamic GPCR signaling events using fluorescence microscopy, FRET imaging, and single-molecule imaging / X. Xu, J.A. Brzostowski and T. Jin -- Imaging actin cytoskeleton dynamics in Dictyostelium chemotaxis / G. Gerisch -- Analysis of actin assembly by in vitro TIRF microscopy / D. Breitsprecher [and others] -- Single-molecule imaging techniques to visualize chemotactic signaling events on the membrane of living Dictyostelium cells / Y. Miyanaga, S. Matsuoka and M. Ueda -- Imaging B-cell receptor signaling by single-molecule techniques / P. Tolar and T. Meckel -- Light microscopy to image and quantify cell movement / D.J. Wessels, S. Kuhl and D.R. Soll -- Mathematics of experimentally generated chemoattractant gradients / M. Postma and P.J. van Haastert -- Modeling spatial and temporal dynamics of chemotactic networks / L. Yang and P.A. Iglesias -- Computational modeling of signaling networks for eukaryotic chemosensing / M. Meier-Schellersheim, F. Klauschen and B. Angermann.

"Fundamental to the development and vital functions of organisms, the migration of motile cells due to the detection of shallow gradients of specific chemical signals in their environments, or chemotaxis, can be clearly seen as a major force in cell biology. In Chemotaxis: Methods and Protocols, expert researchers in the field provide state-of-the-art methods for investigating cell migration behaviors, studying molecular components involved in detecting extracellular signals and directing cell movement, visualizing spatiotemporal dynamics of the components in signaling networks of chemotaxis in real time, and constructing quantitative models that simulate chemoattractant-induced cell responses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls." "Comprehensive and cutting-edge, Chemotaxis: Methods and Protocols serves scientists with practical guidance on the diverse methodologies that are currently propelling chemotaxis research forward in order to further our understanding of this vital biological system."--Jacket

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