Ras signaling : methods and protocols / edited by Lorenza Trabalzini, Saverio Francesco Retta.Material type: TextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1120.Publisher: New York : Humana Press, Copyright date: ©2014Description: 1 online resource (xii, 425 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781627037914; 1627037918; 162703790X; 9781627037907Subject(s): Ras proteins -- Laboratory manuals | Guanosine triphosphate -- Laboratory manuals | Cell physiology | G proteins | Proteins | Carrier proteins | Guanosine triphosphatase | Hydrolases | Enzymes | Ras proteins | Cellular signal transduction | Cell Physiological Phenomena | Monomeric GTP-Binding Proteins | Publication Formats | Biochemical Phenomena | GTP-Binding Proteins | Intracellular Signaling Peptides and Proteins | Chemical Phenomena | Publication Characteristics | Proteins | Carrier Proteins | Peptides | GTP Phosphohydrolases | Phenomena and Processes | Amino Acids, Peptides, and Proteins | Acid Anhydride Hydrolases | Chemicals and Drugs | Hydrolases | Enzymes | Enzymes and Coenzymes | ras Proteins | Signal Transduction | Laboratory Manuals | Protéines ras -- Manuels de laboratoire | Guanosine triphosphate -- Manuels de laboratoire | Cellules -- Physiologie | Protéines G | Protéines | Protéines de liaison | GTPases | Hydrolases | Enzymes | Protéines ras | Transduction du signal cellulaire | protein | enzyme | Guanosine triphosphate | Ras proteins | celbiologie | cellular biology | eiwitexpressieanalyse | proteomics | Cellular Biology | CelbiologieGenre/Form: Electronic books. | Laboratory manuals.Additional physical formats: Print version:: Ras signalingDDC classification: 572/.793 LOC classification: QP609.G83NLM classification: W1 | QU 25Online resources: Click here to access online
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Ras superfamily of small GTPases : the unlocked secrets / Luca Goitre [and others] -- Novel method for the production of fully modified K-Ras 4B / Tanmay S. Chavan [and others] -- Metabolic labeling of ras with tritiated palmitate to monitor palmitoylation and depalmitoylation / Frederick D. Tsai [and others] -- Ubiquitination of Rac1 by inhibitors of apoptosis (IAPs) / Tripat Kaur Oberoi-Khanuja and Krishnaraj Rajalingam -- Ras GTPases are both regulators and effectors of redox agents / Elisa Ferro [and others] -- Biophysical and proteomic characterization strategies for cysteine modifications in Ras GTPases / G. Aaron Hobbs, Harsha P. Gunawardena, and Sharon L. Campbell -- Use of the yeast two-hybrid technology to isolate molecular interactions of Ras GTPases / Elisa Ferro, Eva Baldini, and Lorenza Trabalzini -- Screening for MAPK modulators using an in-cell western assay / Simon Schnaiter [and others] -- Behavioral methods for the study of the Ras-ERK pathway in memory formation and consolidation : passive avoidance and novel object recognition tests / Raffaele d'Isa, Riccardo Brambilla, and Stefania Fasano -- Functional phosphoproteomics of oncogenic KRAS signaling / Putty-Reddy Sudhir and Jeou-Yuan Chen -- Pull-down assay for analysis of integrin-mediated activation of Rap proteins in adherent platelets / Gianni Francesco Guidetti and Mauro Torti -- Combined pulldown and time-lapse microscopy studies for determining the role of Rap1 in the crosstalk between integrins and cadherins / Luca Goitre and Saverio Francesco Retta -- Fluorescence microscopy study of Rap1 subcellular localization / Luca Goitre, Valentina Cutano, and Saverio Francesco Retta -- In vitro system to evaluate the scaffold function of the RalA effector protein RalBP1 / David F. Kashatus -- Analysis of the rit subfamily GTPase-mediated signaling and neuronal differentiation and survival / Geng-Xian Shi and Catherine N. Kaminski -- Immunofluorescence methods in studies of the GTPase ran and its effectors in interphase and in mitotic cells / Giulia Guarguaglini, Valeria de Turris, and Patrizia Lavia -- High-resolution scanning electron microscopy for the imaging of nuclear pore complexes and ran-mediated transport / Lihi Shaulov, Boris Fichtman, and Amnon Harel -- Effector recruitment method to study spatially regulated activation of ras and Rho GTPases / Lauren P. Huff, Molly J. DeCristo, and Adrienne D. Cox -- Real-time visualization and quantification of native Ras activation in single living cells / Christoph Biskup and Ignacio Rubio -- Nanoclustering and heterogeneous membrane diffusion of Ras studied by FRAP and RICS analysis / Camilo Guzmán, Maja Šolman, and Daniel Abankwa -- Analyzing the roles of Rho GTPases in cancer cell migration with a live cell imaging 3D-morphology-based assay / Audrey Colomba and Anne J. Ridley -- Analysis of Rho GTPase-induced localization of nanoscale adhesions using fluorescence nanoscopy / Annica K.B. Gad [and others] -- Yeast as a model for ras signalling / Renata Tisi, Fiorella Belotti, and Enzo Martegani -- Methods to study the Ras2 protein activation state and the subcellular localization of Ras-GTP in Saccharomyces cerevisiae / Sonia Colombo and Enzo Martegani -- Ras proteins signaling in the early metazoan Dictyostelium discoideum / Enrico Bracco and Barbara Pergolizzi.
Includes bibliographical references and index.
Featuring experimental approaches that shed light on the complexity of Ras GTPase biological functions, Ras Signaling: Methods and Protocols contains general overviews and detailed applications of both well-established and recently developed research techniques, including biochemical, biophysical, molecular biology, genetic and behavioral approaches, advanced high resolution fluorescence and electron microscopy imaging, and omics technologies. Through this, the detailed volume provides information on expression, post-translational modifications, subcellular localization and dynamics, regulatory mechanisms of upstream and downstream signaling pathways, and, ultimately, biological activities and functions of RasGTPases in different model systems, including high and low eukaryotic organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Wide-ranging and authoritative, Ras Signaling: Methods and Protocols serves as an aid for investigators of different backgrounds and interests related to the multiple physiological and pathological functions of the large superfamily of RasGTPases.
Print version record.