Platelets and megakaryocytes. Volume 4, Advanced protocols and perspectives / edited by Jonathan M. Gibbins, Martyn Mahaut-Smith.Material type: TextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 1812.Publisher: New York, NY : Humana Press, 2018Description: 1 online resource (xi, 290 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781493985852; 149398585XOther title: Advanced protocols and perspectivesSubject(s): Blood platelets -- Laboratory manuals | Megakaryocytes -- Laboratory manuals | Science -- Life Sciences -- Biology -- Molecular Biology | Cellular biology (cytology) | Blood platelets | MegakaryocytesGenre/Form: Electronic books. | Laboratory manuals.DDC classification: 573.1/59 LOC classification: QP97Online resources: Click here to access online
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Includes bibliographical references and index.
Immobilization of nonactivated unfixed platelets for real-time single-cell analysis / Alexander P. Bye, Zeki Ilkan, Amanda J. Unsworth, and Chris I. Jones -- Imaging platelets and megakaryocytes by high-resolution laser fluorescence microscopy / Fred G. Pluthero and Walter H.A. Kahr -- Single-molecule localization and structured illumination microscopy of platelet proteins / Natalie S. Poulter, Abdullah O. Khan, Chiara Pallini, and Steven G. Thomas -- Electron tomography and correlative approaches in platelet studies / Kasia B. Engberts, Cor Seinen, Willie J.C. Geerts, and Harry F.G. Heijnen -- Screening and high-throughput platelet assays / Alexander P. Bye, Amanda J. Unsworth, and Jonathan M. Gibbins -- High-throughput signaling profiling in blood platelets by multiplexed phosphoflow cytometry / Benjamin E.J. Spurgeon and Khalid M. Naseem -- Precise quantification of platelet proteins and their phosphorylation states / Francoise Mazet and Michael J. Fry -- Study of platelet receptors using artificial lipid bilayers / Michael L. Dustin and Alice Y. Pollitt -- Three-dimensional culture in a methylcellulose-based hydrogel to study the impact of stiffness on megakaryocyte differentiation / Alicia Aguilar, Julie Boscher, Fabien Pertuy, Christian Gachet, and Catherine Léon -- Differentiation of human pluripotent stem cells to megakaryocytes by transcription factor-driven forward programming / Thomas Moreau, Amanda L. Evans, and Cedric J.G. Ghevaert -- Three-dimensional tissue models for studying ex vivo megakaryocytopoiesis and platelet production / Christian A. Di Buduo, Vittorio Abbonante, Lorenzo Tozzi, David L. Kaplan, and Alessandra Balduini -- Fluorescence approaches to image and quantify the demarcation membrane system in living megakaryocytes / Sangar Osman, Daniel Dalmay, and Martyn Mahaut-Smith -- High-resolution 3D imaging of megakaryocytes using focused ion beam-scanning electron microscopy / Anita Eckly, Jean-Yves Rinckel, Fabienne Proamer, and Christian Gachet -- Optical clearing of murine bones to study megakaryocytes in intact bone marrow using light-sheet fluorescence microscopy / Maximilian G. Gorelashvili, Katrin G. Heinze, and David Stegner -- Mathematical techniques for understanding platelet regulation and the development of new pharmacological approaches / Joanna L. Dunster, Mikhail A. Panteleev, Jonathan M. Gibbins, and Anastacia N. Sveshnikova.
Online resource; title from PDF title page (SpringerLink, viewed September 5, 2018).