TY - BOOK AU - Stoddart,Martin J. TI - Mammalian cell viability: methods and protocols T2 - Methods in molecular biology, SN - 9781617791086 AV - QH582.4 .M36 2011 U1 - 571.6 22 PY - 2011/// CY - New York PB - Springer KW - Cells KW - Physiology KW - Analysis KW - Aging KW - Cell death KW - Mammals KW - Cell Survival KW - physiology KW - fast KW - celbiologie KW - cellular biology KW - celkweek KW - cell culture KW - levenswetenschappen KW - life sciences KW - Cellular Biology KW - Celbiologie KW - Electronic books N1 - Includes bibliographical references and index; Cell viability assays : introduction; Martin J. Stoddart --; Cell viability analysis using trypan blue : manual and automated methods; Kristine S. Louis and Andre C. Siegel --; Estimation of cell number based on metabolic activity : the MTT reduction assay; Laszlo Kupcsik --; WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells; Martin J. Stoddart --; Assessment of cell proliferation with resazurin-based fluorescent dye; Ewa M. Czekanska --; xCELLigence system for real-time and label-free monitoring of cell viability; Ning Ke [and others] --; Analysis of tumor and endothelial cell viability and survival using sulforhodamine B and clonogenic assays; Caroline Woolston and Stewart Martin --; Annexin V/7-AAD staining in keratinocytes; Maya Zimmermann and Norbert Meyer --; Measurement of caspase activity : from cell populations to individual cells; Gabriela Paroni and Claudio Brancolini --; Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes; Donald Wlodkowic, Joanna Skommer, and Zbigniew Darzynkiewicz --; Multiplexing cell viability assays; Helga H.J. Gerets, Stephane Dhalluin, and Franck A. Atienzar --; Cytotoxicity testing : measuring viable cells, dead cells, and detecting mechanism of cell death; Terry L. Riss, Richard A. Moravec, and Andrew L. Niles --; Fluorescein diacetate for determination of cell viability in 3D fibroblast-collagen-GAG constructs; Heather M. Powell, Alexis D. Armour, and Steven T. Boyce --; Confocal imaging protocols for live/dead staining in three-dimensional carriers; Benjamin Gantenbein-Ritter . [and others] --; Viability assessment of osteocytes using histological lactate dehydrogenase activity staining on human cancellous bone sections; Katharina Jahn and Martin J. Stoddart --; Measuring glutamate receptor activation-induced apoptotic cell death in ischemic rat retina using the TUNEL assay; Won-Kyu Ju and Keun-Young Kim --; Exploiting the liberation of Zn2+ to measure cell viability; Christian J. Stork and Yang V. Li --; Noninvasive bioluminescent quantification of viable stem cells in engineered constructs; Karim Oudina, Adeline Cambon-Binder, and Delphine Logeart-Avramoglou --; Raman micro-spectroscopy as a non-invasive cell viability test; Sophie Verrier, Alina Zoladek, and Ioan Notingher --; Closed ampoule isothermal microcalorimetry for continuous real-time detection and evaluation of cultured mammalian cell activity and responses; Olivier Braissant and Alma U. "Dan" Daniels --; Digital image processing of live/dead staining; Pieter Spaepen [and others] UR - https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-61779-108-6 ER -