TY - BOOK AU - Taxman,Debra J. TI - SiRNA design: methods and protocols T2 - Methods in molecular biology, SN - 9781627031196 AV - QP623.5.S63 U1 - 660.6 23 PY - 2013/// CY - New York PB - Humana Press, Springer [distributor] KW - Small interfering RNA KW - Investigative Techniques KW - RNA, Small Untranslated KW - RNA, Antisense KW - Publication Formats KW - Genetic Techniques KW - Epigenesis, Genetic KW - RNA KW - Publication Characteristics KW - Biology KW - Antisense Elements (Genetics) KW - Gene Expression Regulation KW - Analytical, Diagnostic and Therapeutic Techniques and Equipment KW - Biological Science Disciplines KW - Nucleic Acids, Nucleotides, and Nucleosides KW - RNA, Untranslated KW - Genetic Phenomena KW - Disciplines and Occupations KW - Chemicals and Drugs KW - Phenomena and Processes KW - Natural Science Disciplines KW - RNA, Small Interfering KW - Nucleic Acids KW - Genetics KW - Methods KW - Gene Targeting KW - Gene Silencing KW - Laboratory Manuals KW - fast KW - chemie KW - chemistry KW - biotechnologie KW - biotechnology KW - humane genetica KW - human genetics KW - Chemistry (General) KW - Chemie (algemeen) KW - Laboratory Manual KW - Electronic books N1 - Includes bibliographical references and index; What parameters to consider and which software tools to use for target selection and molecular design of small interfering RNAs; Olga Matveeva --; Methods for selecting effective siRNA target sequences using a variety of statistical and analytical techniques; Shigeru Takasaki --; Designing functional siRNA with reduced off-target effects; Yuki Naito and Kumiko Ui-Tei --; Design and screening of siRNAs against highly structured RNA targets; Neda Nasheri, John Paul Pezacki, and Selena M. Sagan --; Engineering small interfering RNAs by strategic chemical modification; Jesper B. Bramsen and Jørgen Kjems --; Design, selection, and evaluation of highly specific and functional siRNA incorporating unlocked nucleobase analogs; Narendra Vaish and Pinky Agarwal --; Design, preparation and evaluation of asymmetric small interfering RNA for specific gene silencing in mammalian cells; Chanil Chang [and others] --; Design of nuclease-resistant fork-like small interfering RNA (fsiRNA); Elena L. Chernolovskaya and Marina A. Zenkova --; Designing dual-targeting siRNA duplexes having two active strands that combine siRNA and MicroRNA-like targeting; Pal Sætrom --; Strategies for designing and Validating immunostimulatory siRNAs; Michael P. Gantier --; Designing efficient and specific endoribonuclease-prepared siRNAs; Vineeth Surendranath [and others] --; Short hairpin RNA-mediated gene silencing; Luke S. Lambeth and Craig A. Smith --; Design of lentivirally expressed siRNAs; Ying Poi Liu and Ben Berkhout --; Bifunctional short hairpin RNA (bi-shRNA) : design and pathway to clinical application; Donald D. Rao [and others] --; Design and chemical modification of synthetic short shRNAs as potent RNAi triggers; Anne Dallas and Brian H. Johnston --; Production and application of long dsRNA in mammalian cells; Katerina Chalupnikova, Jana Nejepinska, and Petr Svoboda --; Design of RNAi reagents for invertebrate model organisms and human disease vectors; Thomas Horn and Michael Boutros --; Construction of shRNA expression plasmids for silkworm cell lines using single-stranded DNA and Bst DNA polymerase; Hiromitsu Tanaka --; Designing effective amiRNA and multimeric amiRNA against plant viruses; Muhammad Fahim and Philip J. Larkin --; Downregulation of plant genes with miRNA-induced gene silencing; Felipe Fenselau de Felippes N2 - The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance. The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume. Written for the highly successful Methods in Molecular Biology series, this work contains the kind of detailed description and implementation advice that guarantees successful results. Authoritative and easy to use, siRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design UR - https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-62703-119-6 ER -