TY  - BOOK
AU  - Turksen,Kursad
TI  - Stem cells and aging: methods and protocols 
T2  - Methods in molecular biology,
SN  - 9781627033176
AV  - QH588.S83 S74 2013
U1  - 616.02/774 23
PY  - 2013///
CY  - New York
PB  - Humana Press, Springer
KW  - Stem cells
KW  - Laboratory manuals
KW  - Aging
KW  - Molecular aspects
KW  - Stem Cells
KW  - Biology
KW  - Health & Biological Sciences
KW  - Cytology
KW  - fast
KW  - celbiologie
KW  - cellular biology
KW  - stamcellen
KW  - stem cells
KW  - geneeskunde
KW  - medicine
KW  - Medicine (General)
KW  - Geneeskunde (algemeen)
KW  - Electronic books
N1  - Includes bibliographical references and index; Comprehensive hematopoietic stem cell isolation methods; Kyle Rector, Yi Liu, and Gary Van Zant --; Serial transplantation of bone marrow to test self-renewal capacity of hematopoietic stem cells in vivo; Charusheila Ramkumar, Rachel M. Gerstein, and Hong Zhang --; Mouse hematopoietic stem cell transplantation; Hui Cheng, Paulina H. Liang, and Tao Cheng --; Isolation, characterization, and transplantation of adult liver progenitor cells; Mladen I. Yovchev, Mariana D. Dabeva, and Michael Oertel --; Isolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces; Mitra Lavasani [and others] --; Human myoblasts from skeletal muscle biopsies : in vitro culture preparations for morphological and cytochemical analyses at light and electron microscopy; Manuela Malatesta [and others] --; Cardiac stem cell senescence; Daniela Cesselli [and others] --; Isolation of mesenchymal stem cells from human bone and long-term cultivation under physiologic oxygen conditions; Sebastian Klepsch [and others] --; Methods for assessing effects of Wnt/b-catenin signaling in senescence of mesenchymal stem cells; Hai-jie Wang and Yu-zhen Tan --; Intra-femoral injection of human mesenchymal stem cells; Sindhu T. Mohanty and Ilaria Bellantuono --; Tracking of replicative senescence in mesenchymal stem cells by colony-forming unit frequency; Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner --; Chromatin immunoprecipitation coupled by quantitative real-time PCR as a tool for analyzing epigenetic regulation of stem cell aging; Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang --; Quantitative fluorescence in situ hybridization on paraffin embedded tissue; Mario Ricciardi, Mauro Krampera, and Marco Chilosi
N2  - Over time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome. Stem Cells and Aging: Methods and Protocols brings together chapters from expert contributors with protocols critical for exploring the biology of stem cell aging, all of which is key for understanding these age-related stem cell changes at a basic biology level and at the level of their impacts for regenerative medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy to use, Stem Cells and Aging: Methods and Protocols serves as an ideal reference to guide investigators toward further valuable answers to the problems of our aging population
UR  - https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-62703-317-6
ER  -