TY - BOOK AU - Peterson,Thomas TI - Plant transposable elements: methods and protocols T2 - Methods in molecular biology, SN - 9781627035682 AV - QH452.3 U1 - 572.8/69 23 PY - 2013///] CY - New York PB - Humana Press KW - Transposons KW - Laboratory manuals KW - Plant genetic transformation KW - Publication Formats KW - Biology KW - Interspersed Repetitive Sequences KW - Genome KW - DNA KW - Publication Characteristics KW - Repetitive Sequences, Nucleic Acid KW - Nucleic Acids KW - Genetic Structures KW - Biological Science Disciplines KW - Genome Components KW - Natural Science Disciplines KW - Nucleic Acids, Nucleotides, and Nucleosides KW - Genetic Phenomena KW - Base Sequence KW - Disciplines and Occupations KW - Chemicals and Drugs KW - Phenomena and Processes KW - Molecular Structure KW - Biochemical Phenomena KW - Chemical Phenomena KW - DNA Transposable Elements KW - Genetics KW - Laboratory Manuals KW - Genome, Plant KW - fast KW - Laboratory Manual KW - Electronic books N1 - Includes bibliographical references and index; Historical overview of transposable element research; P.A. Peterson --; Distinguishing variable phenotypes from variegation caused by transposon activities; V. Walbot --; Using transposons for genetic mosaic analysis of plant development; P.W. Becraft --; Survey of natural and transgenic gene markers used to monitor transposon activity; L. Krishnaswamy and T. Peterson --; Molecular biology of maize Ac/Ds elements: An overview; K. Lazarow, M.L. Doll and R. Kunze --; Gene tagging with engineered Ds elements in maize; Y. Li, G. Segal, Q. Wangm H.K. Donner --; Plant regeneration methods for rapid generation of a large scale Ds transposant population in rice; Y.H. Xuan [and nine others] --; Isolation of sequences flanking Ac insertion sites by Ac casting; D. Wang and T. Peterson --; Regulation of the mutator system of transposons in maize; D. Lisch --; Using MuDR/Mu transposons in directed tagging strategies; V. Walbot and J. Questa --; Genetic and molecular analyses of UniformMu transposon insertion lines; D.R. McCarty, M. Suzuki, C. Hunter, J. Collins, W.T. Avigne, K.E. Koch --; Digestion-ligation-amplification (DLA): A simple genome walking method to amplify unknown sequences flanking mutator (Mu) transposons and thereby facilitate gene cloning; S. Liu, A.P. Hsia and P.S. Schnable --; Molecular genetics and epigenetics of CACTA elements; N.V. Fedoroff --; Activation tagging using the maize En-I transposon system for the identification of abiotic stress resistance genes in arabidopsis; A. Harb and A. Pereira --; Reverse genetics in rice using Tos17; D. Mieulet, A. Dievart, G. Droc, N. Lanau, E. Guiderdoni --; Identification and applications of the Petunia Class II Act1/dTph1 transposable element system; T. Gerats, J. Zethof and M. Vandenbussche --; Transposon display: A versatile method for transposon tagging; M. Vandenbussche, J. Zethof and T. Gerats --; Massive indexed parallel identification of transposon flanking sequences; M. Vandenbussche, J. Zethof and T. Gerats --; Use of next generation sequencing (NGS) technologies for the genome-wide detection of transposition; M. Elbaidouri, C. Chaparro and O. Panaud --; Overview of repeat annotation and de novo repeat identification; N. Jiang --; Computational methods for identification of DNA transposons; N. Jiang --; Tenest 2.0: Computational annotation and visualization of nested transposable elements; B.A. Kronmiller and R.P. Wise N2 - Transposable elements have played a major role in shaping plant genome structure and gene expression. Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome. In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient transposon remnants that comprise the bulk of plant genomes, as well as laboratory techniques to identify recent and ongoing transposition events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Transposable Elements: Methods and Protocols seeks to aid scientists in the further study of transposons by providing essential background information and specific experimental protocols UR - https://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-62703-568-2 ER -