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001 ocn776046223
003 OCoLC
005 20220614132116.0
006 m o d
007 cr un||||a|a||
008 120212s1990 caua ob 001 0 eng d
040 _aOCLCE
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020 _a0121820866
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020 _a9780121820862
_q(electronic bk.)
020 _z012287045X
020 _z9780122870453
035 _a(OCoLC)776046223
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042 _adlr
050 4 _aQP601
_b.M49 v.185
060 4 _aW1
_bME9615K v.185 1990
060 4 _aQH 450
_bG3262 1990
070 _aQP601.M49
_bv.185
072 0 _aX300
082 0 4 _a574.87322
_220
084 _a42.13
_2bcl
084 _aVK 8700
_2rvk
084 _aWC 4355
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084 _aWD 5050
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084 _aBIO 180f
_2stub
084 _aCHE 810f
_2stub
084 _aCHE 860f
_2stub
084 _aCHE 885f
_2stub
049 _aMAIN
245 0 0 _aGene expression technology /
_cedited by David V. Goeddel.
260 _aSan Diego :
_bAcademic Press,
_c©1990.
300 _a1 online resource (xxxi, 681 pages) :
_billustrations
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
490 1 _aMethods in enzymology,
_x0076-6879 ;
_vv. 185
504 _aIncludes bibliographical references and indexes.
505 0 0 _tSystems for heterologous gene expression /
_rDavid V. Goeddel and David V. Goeddel --
_tExpression of heterologous proteins in escherichia coli /
_rLarry Gold and David V. Goeddel --
_tDesign and construction of expression plasmid vectors in escherichia coli /
_rPaulina Balbas, Francisco Bolivar and David V. Goeddel --
_tPromoter recognition and mRNA initiation by Escherichia coli E[sigma]70 /
_rJay D. Gralla and David V. Goeddel --
_tUse of Escherichia coli trp promoter for direct expression of proteins /
_rDaniel G. Yansura, Dennis J. Henner and David V. Goeddel --
_tUse of T7 RNA polymerase to direct expression of cloned genes /
_rF. William Studier [and others] --
_tHigh-level translation initiation /
_rLarry Gold, Gary D. Stormo and David V. Goeddel --
_tEnhanced translational efficiency with two-cistron expression system /
_rBrigitte E. Schoner [and others] --
_tSequences within ribosome binding site affecting messenger RNA translatability and method to direct ribosomes to single messenger RNA species /
_rHerman A. De Boer, Anna S. Hui and David V. Goeddel --
_tVector for enhanced translation of foreign genes in Escherichia coli /
_rPeter O. Olins, Shaukat H. Rangwala and David V. Goeddel --
_tMinimizing proteolysis in Escherichia coli: genetic solutions /
_rSusan Gottesman and David V. Goeddel --
_tGene fusions for purpose of expression: An introduction /
_rMathias Uhlén, Tomas Moks and David V. Goeddel --
_tFusions to staphylococcal protein A /
_rBjörn Nilsson, Lars Abrahmsén and David V. Goeddel --
_tExpression as trpE fusion /
_rDaniel G. Yansura and David V. Goeddel --
_tEngineering Escherchia coli to secrete heterologous gene products /
_rJoan A. Stader, Thomas J. Silhavy and David V. Goeddel --
_tRefolding of recombinant proteins /
_rTadahiko Kohno [and others] --
_tExpression of heterologous genes in Bacillus subtilis /
_rDennis J. Henner and David V. Goeddel --
_tRegulated promoter for high-level expression of heterologous genes in Bacillus subtilis /
_rStuart F.J. Le Grice and David V. Goeddel --
_tSystem for secretion of heterologous proteins in Bacillus subtilis /
_rVasantha Nagarajan and David V. Goeddel --
_tInducible expression of regulatory genes in Bacillus subtilis /
_rDennis J. Henner and David V. Goeddel.
505 0 0 _tHeterologous gene expression in yeast /
_rScott D. Emr and David V. Goeddel --
_tPropagation and expression of cloned genes in yeast: 2-[mu]m circle-based vectors /
_rAlan B. Rose, James R. Broach and David V. Goeddel --
_tManipulating yeast genome using plasmid vectors /
_rTim Stearns [and others] --
_tRegulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast /
_rLawrence M. Mylin [and others] --
_tExpression of heterologous proteins in Saccharomyces cerevisiae using the ADH2 promoter /
_rVirginia L. Price [and others] --
_tInduced expression using yeast copper metallothionein promoter /
_rTina Etcheverry and David V. Goeddel --
_tHigh-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene /
_rS.M. Kingsman [and others] --
_tGlyceraldehyde-3-phosphate dehydrogenase-derived expression cassettes for constitutive synthesis of heterologous proteins /
_rSteven Rosenberg [and others] --
_tSuperimposition of temperature regulation on yeast promoters /
_rAndrzej Z. Sledziewski [and others] --
_tSequence and structural requirements for efficient translation in yeast /
_rThomas F. Donahue, Mark A. Cigan and David V. Goeddel --
_tVacuolar proteases in yeast Saccharomyces cerevisiae /
_rElizabeth W. Jones and David V. Goeddel --
_tDetection and inhibition of ubiquitin-dependent proteolysis /
_rKeith D. Wilkinson and David V. Goeddel --
_tContranslational amino-terminal processing /
_rRichard L. Kendall [and others] --
_t[alpha]-Factor leader-directed secretion of heterologous proteins from yeast /
_rAnthony J. Brake and David V. Goeddel --
_tUse of heterologous and homologous signal sequences for secretion of heterologous proteins from yeast /
_rRonald A. Hitzeman [and others] --
_tIsolation, characterization, and properties of Saccharomyces cerevisiae mnn mutants with nonconditional protein glycosylation defects /
_rClinton E. Ballou and David V. Goeddel --
_tMolecular and genetic approach to enhancing protein secretion /
_rVanessa Chisholm [and others].
505 0 0 _tExpression of heterologous genes in mammalian cells /
_rArthur D. Levinson and David V. Goeddel --
_tVectors used for expression in mammalian cells /
_rRandal J. Kaufman and David V. Goeddel --
_tAssembly of enhancers, promoters, and splice signals to control expression of transferred genes /
_rMichael Kriegler and David V. Goeddel --
_tMethods for introducing DNA into mammalian cells /
_rWayne A. Keown [and others] --
_tSelection and coamplification of heterologous genes in mammalian cells /
_rRandal J. Kaufman and David V. Goeddel --
_tOptimizing cell and culture environment for production of recombinant proteins /
_rJennie P. Mather and David V. Goeddel --
_tAnalysis of synthesis, processing, and secretion of proteins expressed in mammalian cells /
_rAndrew J. Dorner, Kaufman Randal J. and David V. Goeddel --
_tSaturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil /
_rBruce H. Horwitz, Daniel Dimaio and David V. Goeddel.
520 _aThe methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter.
588 0 _aPrint version record.
506 _3Use copy
_fRestrictions unspecified
_2star
_5MiAaHDL
533 _aElectronic reproduction.
_b[Place of publication not identified] :
_cHathiTrust Digital Library,
_d2012.
_5MiAaHDL
538 _aMaster and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
_uhttp://purl.oclc.org/DLF/benchrepro0212
_5MiAaHDL
583 1 _adigitized
_c2012
_hHathiTrust Digital Library
_lcommitted to preserve
_2pda
_5MiAaHDL
546 _aEnglish.
650 0 _aGene expression
_xTechnique.
_984560
650 0 _aMolecular cloning.
_980487
650 0 _aGene expression.
_922751
650 0 _aGenetics
_xTechnique.
_984274
650 2 _aCloning, Molecular
_980483
650 2 _aGene Expression
_922751
650 2 _aGenetic Techniques
_92075
650 6 _aExpressivité génétique
_xTechnologie.
_9170536
650 6 _aExpression génique
_xTechnique.
_9966417
650 6 _aClonage moléculaire.
_9152871
650 6 _aExpression génique.
_9101514
650 6 _aGénétique
_xTechnique.
_984554
650 7 _aEnzymes.
_2eclas
_981046
650 7 _aBiochimie.
_2eclas
650 7 _aGene expression
_xTechnique.
_2fast
_0(OCoLC)fst00939618
_984560
650 7 _aMolecular cloning.
_2fast
_0(OCoLC)fst01024762
_980487
650 7 _aGenexpression
_2gnd
_985883
650 7 _aMikroorganismus
_2gnd
_91673
650 7 _aAufsatzsammlung
_2gnd
650 7 _aSäugetiere
_2gnd
_9128722
650 7 _acDNS
_2gnd
_998031
650 7 _aGenklonierung
_2gnd
_9160207
650 7 _aTechnologie
_2gnd
_920942
650 1 7 _aGenexpressie.
_2gtt
_982961
650 1 7 _aWetenschappelijke technieken.
_2gtt
650 1 7 _aLaboratoriumonderzoek.
_2gtt
_980323
650 0 7 _aGenexpression.
_2swd
_985883
650 0 7 _aMikroorganismus.
_2swd
_91673
650 0 7 _aAufsatzsammlung.
_2swd
650 0 7 _aSäugetiere.
_2swd
_9128722
650 0 7 _acDNS.
_2swd
_998031
650 0 7 _aGenklonierung.
_2swd
_9160207
650 0 7 _aTechnologie.
_2swd
_920942
653 0 _aGenetic engineering
655 4 _aElectronic books.
_9396
700 1 _aGoeddel, David V.,
_eeditor.
_9170540
776 0 8 _iPrint version:
_tGene expression technology.
_dSan Diego : Academic Press, ©1990
_z0121820866
_w(OCoLC)22957344
830 0 _aMethods in enzymology ;
_vv. 185.
_x0076-6879
_997896
856 4 0 _uhttps://www-sciencedirect-com.libraryproxy.ist.ac.at/science/bookseries/00766879/185
938 _aInternet Archive
_bINAR
_ngeneexpressionte0000unse
938 _aYBP Library Services
_bYANK
_n12369580
994 _a92
_bATIST
999 _c172167
_d172167