000			07954cam a2200733Ka 4500
001			ocn776046223
003			OCoLC
005			20160315124813.0
006			m o d
007			cr un||||a|a||
008			120212s1990 caua ob 001 0 eng d
040			_aOCLCE _beng _epn _cOCLCE _dCUSER _dUIU _dOPELS _dOCLCQ _dOPELS _dOCLCF _dOCLCQ _dOCLCO _dKUK _dOCLCQ
019			_a56597283 _a326630763 _a441500554
020			_a0121820866 _q(electronic bk.)
020			_a9780121820862 _q(electronic bk.)
035			_a(OCoLC)776046223 _z(OCoLC)56597283 _z(OCoLC)326630763 _z(OCoLC)441500554
050		4	_aQP601 _b.M49 v.185
060		4	_aW1 _bME9615K v.185 1990
060		4	_aQH 450 _bG3262 1990
070			_aQP601.M49 _bv.185
072		0	_aX300
082	0	4	_a574.87322 _220
049			_aMAIN
245	0	0	_aGene expression technology _h[electronic resource] / _cedited by David V. Goeddel.
260			_aSan Diego : _bAcademic Press, _c�1990.
300			_a1 online resource (xxxi, 681 pages) : _billustrations.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
490	1		_aMethods in enzymology ; _vv. 185
500			_aIncludes indexes.
504			_aIncludes bibliographical references.
505	0	0	_tSystems for heterologous gene expression / _rDavid V. Goeddel and David V. Goeddel -- _tExpression of heterologous proteins in escherichia coli / _rLarry Gold and David V. Goeddel -- _tDesign and construction of expression plasmid vectors in escherichia coli / _rPaulina Balbas, Francisco Bolivar and David V. Goeddel -- _tPromoter recognition and mRNA initiation by Escherichia coli E[sigma]70 / _rJay D. Gralla and David V. Goeddel -- _tUse of Escherichia coli trp promoter for direct expression of proteins / _rDaniel G. Yansura, Dennis J. Henner and David V. Goeddel -- _tUse of T7 RNA polymerase to direct expression of cloned genes / _rF. William Studier [and others] -- _tHigh-level translation initiation / _rLarry Gold, Gary D. Stormo and David V. Goeddel -- _tEnhanced translational efficiency with two-cistron expression system / _rBrigitte E. Schoner [and others] -- _tSequences within ribosome binding site affecting messenger RNA translatability and method to direct ribosomes to single messenger RNA species / _rHerman A. De Boer, Anna S. Hui and David V. Goeddel -- _tVector for enhanced translation of foreign genes in Escherichia coli / _rPeter O. Olins, Shaukat H. Rangwala and David V. Goeddel -- _tMinimizing proteolysis in Escherichia coli: genetic solutions / _rSusan Gottesman and David V. Goeddel -- _tGene fusions for purpose of expression: An introduction / _rMathias Uhl�en, Tomas Moks and David V. Goeddel -- _tFusions to staphylococcal protein A / _rBj�orn Nilsson, Lars Abrahms�en and David V. Goeddel -- _tExpression as trpE fusion / _rDaniel G. Yansura and David V. Goeddel -- _tEngineering Escherchia coli to secrete heterologous gene products / _rJoan A. Stader, Thomas J. Silhavy and David V. Goeddel -- _tRefolding of recombinant proteins / _rTadahiko Kohno [and others] -- _tExpression of heterologous genes in Bacillus subtilis / _rDennis J. Henner and David V. Goeddel -- _tRegulated promoter for high-level expression of heterologous genes in Bacillus subtilis / _rStuart F.J. Le Grice and David V. Goeddel -- _tSystem for secretion of heterologous proteins in Bacillus subtilis / _rVasantha Nagarajan and David V. Goeddel -- _tInducible expression of regulatory genes in Bacillus subtilis / _rDennis J. Henner and David V. Goeddel.
505	0	0	_tHeterologous gene expression in yeast / _rScott D. Emr and David V. Goeddel -- _tPropagation and expression of cloned genes in yeast: 2-[mu]m circle-based vectors / _rAlan B. Rose, James R. Broach and David V. Goeddel -- _tManipulating yeast genome using plasmid vectors / _rTim Stearns [and others] -- _tRegulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast / _rLawrence M. Mylin [and others] -- _tExpression of heterologous proteins in Saccharomyces cerevisiae using the ADH2 promoter / _rVirginia L. Price [and others] -- _tInduced expression using yeast copper metallothionein promoter / _rTina Etcheverry and David V. Goeddel -- _tHigh-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene / _rS.M. Kingsman [and others] -- _tGlyceraldehyde-3-phosphate dehydrogenase-derived expression cassettes for constitutive synthesis of heterologous proteins / _rSteven Rosenberg [and others] -- _tSuperimposition of temperature regulation on yeast promoters / _rAndrzej Z. Sledziewski [and others] -- _tSequence and structural requirements for efficient translation in yeast / _rThomas F. Donahue, Mark A. Cigan and David V. Goeddel -- _tVacuolar proteases in yeast Saccharomyces cerevisiae / _rElizabeth W. Jones and David V. Goeddel -- _tDetection and inhibition of ubiquitin-dependent proteolysis / _rKeith D. Wilkinson and David V. Goeddel -- _tContranslational amino-terminal processing / _rRichard L. Kendall [and others] -- _t[alpha]-Factor leader-directed secretion of heterologous proteins from yeast / _rAnthony J. Brake and David V. Goeddel -- _tUse of heterologous and homologous signal sequences for secretion of heterologous proteins from yeast / _rRonald A. Hitzeman [and others] -- _tIsolation, characterization, and properties of Saccharomyces cerevisiae mnn mutants with nonconditional protein glycosylation defects / _rClinton E. Ballou and David V. Goeddel -- _tMolecular and genetic approach to enhancing protein secretion / _rVanessa Chisholm [and others].
505	0	0	_tExpression of heterologous genes in mammalian cells / _rArthur D. Levinson and David V. Goeddel -- _tVectors used for expression in mammalian cells / _rRandal J. Kaufman and David V. Goeddel -- _tAssembly of enhancers, promoters, and splice signals to control expression of transferred genes / _rMichael Kriegler and David V. Goeddel -- _tMethods for introducing DNA into mammalian cells / _rWayne A. Keown [and others] -- _tSelection and coamplification of heterologous genes in mammalian cells / _rRandal J. Kaufman and David V. Goeddel -- _tOptimizing cell and culture environment for production of recombinant proteins / _rJennie P. Mather and David V. Goeddel -- _tAnalysis of synthesis, processing, and secretion of proteins expressed in mammalian cells / _rAndrew J. Dorner, Kaufman Randal J. and David V. Goeddel -- _tSaturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil / _rBruce H. Horwitz, Daniel Dimaio and David V. Goeddel.
520			_aThe methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter.
588	0		_aPrint version record.
590			_aElsevier _bScienceDirect All Books
650		0	_aGene expression _xTechnique.
650		0	_aMolecular cloning.
650		2	_aCloning, Molecular.
650		2	_aGene Expression.
650		2	_aGenetic Techniques.
650		6	_aExpressivit�e g�en�etique _xTechnologie.
650		7	_aGene expression _xTechnique. _2fast _0(OCoLC)fst00939618
650		7	_aMolecular cloning. _2fast _0(OCoLC)fst01024762
650	1	7	_aGenexpressie. _2gtt
650	1	7	_aWetenschappelijke technieken. _2gtt
650	1	7	_aLaboratoriumonderzoek. _2gtt
650	0	7	_aGenexpression. _2swd
650	0	7	_aMikroorganismus. _2swd
650	0	7	_aAufsatzsammlung. _2swd
650	0	7	_aS�augetiere. _2swd
650	0	7	_acDNS. _2swd
650	0	7	_aGenklonierung. _2swd
650	0	7	_aTechnologie. _2swd
653	0		_aGenetic engineering
655		4	_aElectronic books.
700	1		_aGoeddel, David V.
776	0	8	_iPrint version: _w(OCoLC)22957344
830		0	_aMethods in enzymology ; _vv. 185.
856	4	0	_uhttp://www.sciencedirect.com/science/bookseries/00766879/185
994			_a92 _bATIST
999			_c172167 _d172167