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020 _a9781592596836
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020 _a1592596835
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020 _a1280831588
020 _a9781280831584
020 _a9786610831586
020 _a6610831580
020 _z9781617371462
020 _z0896037134
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020 _z9780896037137
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024 7 _a10.1385/1592596835.
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029 1 _aAU@
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029 1 _aNZ1
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035 _a(OCoLC)170932237
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050 4 _aQP364.7
_b.N635 1999eb
060 4 _aW1
_bME9616J v.128 1999
060 4 _aWL 102.8
_bN7384 1999
070 _aQH506.M45
_bv.128
072 7 _aSCI
_x049000
_2bisacsh
072 0 _aX300
072 7 _aPSAN.
_2bicssc
082 0 4 _a572.8
_222
049 _aMAIN
245 0 0 _aNMDA receptor protocols /
_cedited by Min Li.
260 _aTotowa, N.J. :
_bHumana Press,
_c©1999.
300 _a1 online resource (x, 196 pages) :
_billustrations
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
490 1 _aMethods in molecular biology ;
_vv. 128
504 _aIncludes bibliographical references and index.
505 0 _aIsolation of receptor clones by expression screening in xenopus oocytes / F. Nakamura [and others] -- Expression of nmda receptor channel subunit proteins using baculovirus and herpesvirus vectors / S. Kawamoto [and others] -- Transient expression of functional nmda receptors in mammalian cells / P.L. Chazot, M. Cik and F.A. Stephenson -- Stable expression of human nmda receptors in cultured mammalian cells / M.A. Varney [and others] -- Expression of extracellular n-terminal domain of nmda receptor in mammalian cells / E.A. Whitehorn, W.J. Dower and M. Li -- Immunocytochemistry of nmda receptors / R.S. Petralia and R.J. Wenthold -- The use of ligand binding in assays of nmda receptor function / I.J. Reynolds and T.A. Sharma -- Calmodulin modification of nmda receptors / S. Zhang and R.L. Huganir -- Detergent solubilization and immunoprecipitation of native nmda receptors / R.J. Wenthold [and others] -- Redox sensitivity of nmda receptors / S.A. Lipton -- In vitro selection of peptides acting on nmda receptors / S.E. Cwirla, W.J. Dower and M. Li -- Nmda receptor pharmacology and analysis of patch-clamp recordings / J.M. Wright and R.W. Peoples -- Determination of membrane topology of glutamate receptors / H.M. VanDongen and A.M. VanDongen -- Engineering the nmda receptor channel lining / A.V. Ferrer-Montiel and M. Montal -- High-throughput functional detection of nmda receptor activity / M.D. Cunningham and S.M. Cunningham.
588 0 _aPrint version record.
520 _aIn NMDA Receptor Protocols, Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane receptors or signaling receptors. NMDA Receptor Protocols provides today's scientists at all skill levels a diverse collection of highly reproducible molecular techniques for ion channel research and biotherapeutic development. Eminently practical and reproducible, these techniques offer powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.
546 _aEnglish.
650 0 _aMethyl aspartate
_xReceptors
_vLaboratory manuals.
_983058
650 0 _aMethyl aspartate.
_983059
650 0 _aNeurotransmitter receptors.
_92130
650 2 _aGenetics.
_92539
650 2 _aReceptors, N-Methyl-D-Aspartate.
_983060
650 2 _aReceptors, Ionotropic Glutamate.
_983061
650 2 _aBiology.
_94937
650 2 _aBiological Science Disciplines.
_912496
650 2 _aLigand-Gated Ion Channels.
_983062
650 2 _aReceptors, Glutamate.
_983063
650 2 _aIon Channels.
_982312
650 2 _aNatural Science Disciplines.
_912499
650 2 _aReceptors, Amino Acid.
_983064
650 2 _aReceptors, Neurotransmitter.
_92134
650 2 _aMembrane Glycoproteins.
_982310
650 2 _aMembrane Transport Proteins.
_982311
650 2 _aDisciplines and Occupations
_912501
650 2 _aReceptors, Cell Surface.
_983065
650 2 _aMembrane Proteins.
_980240
650 2 _aCarrier Proteins.
_980391
650 2 _aProteins.
_979920
650 2 _aAmino Acids, Peptides, and Proteins
_979921
650 2 _aChemicals and Drugs
_913222
650 7 _aSCIENCE
_xLife Sciences
_xMolecular Biology.
_2bisacsh
_919012
650 7 _aMethyl aspartate.
_2fast
_0(OCoLC)fst01018741
_983059
650 7 _aMethyl aspartate
_xReceptors.
_2fast
_0(OCoLC)fst01018742
_983066
650 7 _aNeurotransmitter receptors.
_2fast
_0(OCoLC)fst01036563
_92130
650 1 7 _aNMDA-receptors.
_2gtt
_983067
650 1 7 _aWetenschappelijke technieken.
_2gtt
_9957261
650 7 _aAminoácidos.
_2larpcal
_980897
650 7 _aBioquímica.
_2larpcal
_981528
653 0 0 _acelmembranen
653 0 0 _acell membranes
653 0 0 _areceptoren
653 0 0 _areceptors
653 0 0 _aglutamaten
653 0 0 _aglutamates
653 0 0 _asynapsis
653 0 0 _aneurologie
653 0 0 _aneurology
653 0 0 _abiologische technieken
653 0 0 _abiological techniques
653 0 0 _aprotocollen
653 0 0 _aprotocols
653 1 0 _aMolecular Biology (General)
653 1 0 _aMoleculaire biologie (algemeen)
655 4 _aElectronic books.
_9396
655 7 _aLaboratory manuals.
_2fast
_0(OCoLC)fst01920776
_9957262
700 1 _aLi, Min,
_d1962-
_983070
773 0 _tSpringer Protocols
776 0 8 _iPrint version:
_tNMDA receptor protocols.
_dTotowa, N.J. : Humana Press, ©1999
_z0896037134
_z9780896037137
_w(DLC) 98053445
_w(OCoLC)40453084
830 0 _aMethods in molecular biology (Clifton, N.J.) ;
_vv. 128.
_9957263
856 4 0 _uhttps://link-springer-com.libraryproxy.ist.ac.at/10.1385/1592596835
938 _aebrary
_bEBRY
_nebr10181840
938 _aEBSCOhost
_bEBSC
_n201483
938 _aInternet Archive
_bINAR
_nnmdareceptorprot00minl
938 _aYBP Library Services
_bYANK
_n2704358
994 _a92
_bATIST
999 _c390151
_d390151