| 000 | 08077cam a2201237Ia 4500 | ||
|---|---|---|---|
| 001 | ocn170932237 | ||
| 003 | OCoLC | ||
| 005 | 20220204092602.0 | ||
| 006 | m o d | ||
| 007 | cr cnu---unuuu | ||
| 008 | 070911s1999 njua ob 001 0 eng d | ||
| 040 |
_aN$T _beng _epn _cN$T _dYDXCP _dOCLCQ _dYUS _dYNG _dCUV _dCNTRU _dGW5XE _dIDEBK _dOCLCQ _dCUS _dOCLCQ _dE7B _dOCLCO _dOCLCQ _dOCLCA _dOCLCF _dVVL _dOCLCQ _dCOO _dAZU _dOCLCQ _dEUW _dEBLCP _dOCLCQ _dOCL _dVT2 _dOCLCA _dOTZ _dOCLCQ _dOCLCA _dBUF _dOCLCO _dINARC _dCASUM _dMERER _dOCLCQ _dU3W _dOCLCO _dOCLCQ _dYOU _dUWO _dCANPU _dAU@ _dLIP _dOCLCQ _dOCLCO _dLEAUB _dOCLCQ _dOCLCO _dOCLCQ _dHS0 _dGW5XE _dUKBTH _dWURST _dOCLCQ _dOCLCA _dU3G _dOCLCA _dOCLCQ |
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| 019 |
_a55028426 _a234537005 _a320968557 _a642460848 _a648274921 _a771203236 _a923699199 _a964911162 _a967612799 _a990543449 _a994758557 _a1005797203 _a1036787029 _a1058943729 _a1069528814 _a1078839139 _a1078854446 _a1081259111 _a1086895985 _a1109065579 _a1113547649 _a1120896309 _a1228573901 _a1264850460 |
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| 020 |
_a9781592596836 _q(electronic bk.) |
||
| 020 |
_a1592596835 _q(electronic bk.) |
||
| 020 | _a1280831588 | ||
| 020 | _a9781280831584 | ||
| 020 | _a9786610831586 | ||
| 020 | _a6610831580 | ||
| 020 | _z9781617371462 | ||
| 020 |
_z0896037134 _q(acid-free paper) |
||
| 020 |
_z9780896037137 _q(acid-free paper) |
||
| 024 | 7 |
_a10.1385/1592596835. _2doi |
|
| 029 | 1 |
_aAU@ _b000061130043 |
|
| 029 | 1 |
_aNZ1 _b15212026 |
|
| 035 |
_a(OCoLC)170932237 _z(OCoLC)55028426 _z(OCoLC)234537005 _z(OCoLC)320968557 _z(OCoLC)642460848 _z(OCoLC)648274921 _z(OCoLC)771203236 _z(OCoLC)923699199 _z(OCoLC)964911162 _z(OCoLC)967612799 _z(OCoLC)990543449 _z(OCoLC)994758557 _z(OCoLC)1005797203 _z(OCoLC)1036787029 _z(OCoLC)1058943729 _z(OCoLC)1069528814 _z(OCoLC)1078839139 _z(OCoLC)1078854446 _z(OCoLC)1081259111 _z(OCoLC)1086895985 _z(OCoLC)1109065579 _z(OCoLC)1113547649 _z(OCoLC)1120896309 _z(OCoLC)1228573901 _z(OCoLC)1264850460 |
||
| 050 | 4 |
_aQP364.7 _b.N635 1999eb |
|
| 060 | 4 |
_aW1 _bME9616J v.128 1999 |
|
| 060 | 4 |
_aWL 102.8 _bN7384 1999 |
|
| 070 |
_aQH506.M45 _bv.128 |
||
| 072 | 7 |
_aSCI _x049000 _2bisacsh |
|
| 072 | 0 | _aX300 | |
| 072 | 7 |
_aPSAN. _2bicssc |
|
| 082 | 0 | 4 |
_a572.8 _222 |
| 049 | _aMAIN | ||
| 245 | 0 | 0 |
_aNMDA receptor protocols / _cedited by Min Li. |
| 260 |
_aTotowa, N.J. : _bHumana Press, _c©1999. |
||
| 300 |
_a1 online resource (x, 196 pages) : _billustrations |
||
| 336 |
_atext _btxt _2rdacontent |
||
| 337 |
_acomputer _bc _2rdamedia |
||
| 338 |
_aonline resource _bcr _2rdacarrier |
||
| 490 | 1 |
_aMethods in molecular biology ; _vv. 128 |
|
| 504 | _aIncludes bibliographical references and index. | ||
| 505 | 0 | _aIsolation of receptor clones by expression screening in xenopus oocytes / F. Nakamura [and others] -- Expression of nmda receptor channel subunit proteins using baculovirus and herpesvirus vectors / S. Kawamoto [and others] -- Transient expression of functional nmda receptors in mammalian cells / P.L. Chazot, M. Cik and F.A. Stephenson -- Stable expression of human nmda receptors in cultured mammalian cells / M.A. Varney [and others] -- Expression of extracellular n-terminal domain of nmda receptor in mammalian cells / E.A. Whitehorn, W.J. Dower and M. Li -- Immunocytochemistry of nmda receptors / R.S. Petralia and R.J. Wenthold -- The use of ligand binding in assays of nmda receptor function / I.J. Reynolds and T.A. Sharma -- Calmodulin modification of nmda receptors / S. Zhang and R.L. Huganir -- Detergent solubilization and immunoprecipitation of native nmda receptors / R.J. Wenthold [and others] -- Redox sensitivity of nmda receptors / S.A. Lipton -- In vitro selection of peptides acting on nmda receptors / S.E. Cwirla, W.J. Dower and M. Li -- Nmda receptor pharmacology and analysis of patch-clamp recordings / J.M. Wright and R.W. Peoples -- Determination of membrane topology of glutamate receptors / H.M. VanDongen and A.M. VanDongen -- Engineering the nmda receptor channel lining / A.V. Ferrer-Montiel and M. Montal -- High-throughput functional detection of nmda receptor activity / M.D. Cunningham and S.M. Cunningham. | |
| 588 | 0 | _aPrint version record. | |
| 520 | _aIn NMDA Receptor Protocols, Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane receptors or signaling receptors. NMDA Receptor Protocols provides today's scientists at all skill levels a diverse collection of highly reproducible molecular techniques for ion channel research and biotherapeutic development. Eminently practical and reproducible, these techniques offer powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds. | ||
| 546 | _aEnglish. | ||
| 650 | 0 |
_aMethyl aspartate _xReceptors _vLaboratory manuals. _983058 |
|
| 650 | 0 |
_aMethyl aspartate. _983059 |
|
| 650 | 0 |
_aNeurotransmitter receptors. _92130 |
|
| 650 | 2 |
_aGenetics. _92539 |
|
| 650 | 2 |
_aReceptors, N-Methyl-D-Aspartate. _983060 |
|
| 650 | 2 |
_aReceptors, Ionotropic Glutamate. _983061 |
|
| 650 | 2 |
_aBiology. _94937 |
|
| 650 | 2 |
_aBiological Science Disciplines. _912496 |
|
| 650 | 2 |
_aLigand-Gated Ion Channels. _983062 |
|
| 650 | 2 |
_aReceptors, Glutamate. _983063 |
|
| 650 | 2 |
_aIon Channels. _982312 |
|
| 650 | 2 |
_aNatural Science Disciplines. _912499 |
|
| 650 | 2 |
_aReceptors, Amino Acid. _983064 |
|
| 650 | 2 |
_aReceptors, Neurotransmitter. _92134 |
|
| 650 | 2 |
_aMembrane Glycoproteins. _982310 |
|
| 650 | 2 |
_aMembrane Transport Proteins. _982311 |
|
| 650 | 2 |
_aDisciplines and Occupations _912501 |
|
| 650 | 2 |
_aReceptors, Cell Surface. _983065 |
|
| 650 | 2 |
_aMembrane Proteins. _980240 |
|
| 650 | 2 |
_aCarrier Proteins. _980391 |
|
| 650 | 2 |
_aProteins. _979920 |
|
| 650 | 2 |
_aAmino Acids, Peptides, and Proteins _979921 |
|
| 650 | 2 |
_aChemicals and Drugs _913222 |
|
| 650 | 7 |
_aSCIENCE _xLife Sciences _xMolecular Biology. _2bisacsh _919012 |
|
| 650 | 7 |
_aMethyl aspartate. _2fast _0(OCoLC)fst01018741 _983059 |
|
| 650 | 7 |
_aMethyl aspartate _xReceptors. _2fast _0(OCoLC)fst01018742 _983066 |
|
| 650 | 7 |
_aNeurotransmitter receptors. _2fast _0(OCoLC)fst01036563 _92130 |
|
| 650 | 1 | 7 |
_aNMDA-receptors. _2gtt _983067 |
| 650 | 1 | 7 |
_aWetenschappelijke technieken. _2gtt _9957261 |
| 650 | 7 |
_aAminoácidos. _2larpcal _980897 |
|
| 650 | 7 |
_aBioquímica. _2larpcal _981528 |
|
| 653 | 0 | 0 | _acelmembranen |
| 653 | 0 | 0 | _acell membranes |
| 653 | 0 | 0 | _areceptoren |
| 653 | 0 | 0 | _areceptors |
| 653 | 0 | 0 | _aglutamaten |
| 653 | 0 | 0 | _aglutamates |
| 653 | 0 | 0 | _asynapsis |
| 653 | 0 | 0 | _aneurologie |
| 653 | 0 | 0 | _aneurology |
| 653 | 0 | 0 | _abiologische technieken |
| 653 | 0 | 0 | _abiological techniques |
| 653 | 0 | 0 | _aprotocollen |
| 653 | 0 | 0 | _aprotocols |
| 653 | 1 | 0 | _aMolecular Biology (General) |
| 653 | 1 | 0 | _aMoleculaire biologie (algemeen) |
| 655 | 4 |
_aElectronic books. _9396 |
|
| 655 | 7 |
_aLaboratory manuals. _2fast _0(OCoLC)fst01920776 _9957262 |
|
| 700 | 1 |
_aLi, Min, _d1962- _983070 |
|
| 773 | 0 | _tSpringer Protocols | |
| 776 | 0 | 8 |
_iPrint version: _tNMDA receptor protocols. _dTotowa, N.J. : Humana Press, ©1999 _z0896037134 _z9780896037137 _w(DLC) 98053445 _w(OCoLC)40453084 |
| 830 | 0 |
_aMethods in molecular biology (Clifton, N.J.) ; _vv. 128. _9957263 |
|
| 856 | 4 | 0 | _uhttps://link-springer-com.libraryproxy.ist.ac.at/10.1385/1592596835 |
| 938 |
_aebrary _bEBRY _nebr10181840 |
||
| 938 |
_aEBSCOhost _bEBSC _n201483 |
||
| 938 |
_aInternet Archive _bINAR _nnmdareceptorprot00minl |
||
| 938 |
_aYBP Library Services _bYANK _n2704358 |
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| 994 |
_a92 _bATIST |
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| 999 |
_c390151 _d390151 |
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