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024 7 _a10.1007/978-1-61779-188-8
_2doi
035 _a(OCoLC)746342570
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050 4 _aQH345
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072 7 _aPSB
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072 7 _aSCI007000
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082 0 4 _a572.8/633
_223
049 _aMAIN
245 0 0 _aTherapeutic oligonucleotides :
_bmethods and protocols /
_cedited by John Goodchild.
260 _aNew York :
_bHumana Press :
_bSpringer,
_c©2011.
300 _a1 online resource (xi, 340 pages) :
_billustrations
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_2rda
490 1 _aMethods in molecular biology,
_x1064-3745 ;
_v764
490 1 _aSpringer protocols
588 0 _aPrint version record.
504 _aIncludes bibliographical references and index.
505 0 0 _tTherapeutic oligonucleotides /
_rJohn Goodchild --
_tDinucleotides containing 3-S-phosphorothiolate linkages /
_rJames W. Gaynor and Richard Cosstick --
_t2-O,4-C-methyleneoxymethylene bridged nucleic acids (2,4-BNACOC) /
_rYoshiyuki Hari [and others] --
_tNon-covalent peptide-based strategy for ex vivo and in vivo oligonucleotide delivery /
_rLaurence Crombez [and others] --
_tCell-penetrating peptides-based strategies for the delivery of splice redirecting antisense oligonucleotides /
_rSamir El Andaloussi [and others] --
_tNanoparticle for tumor targeted delivery of oligomers /
_rXinrong Liu, Yi Wang, and Donald J. Hnatowich --
_tLight-directed delivery of nucleic acids /
_rSigurd Bøe [and others] --
_tAntibody targeted siRNA delivery /
_rMasoud M. Toloue and Lance P. Ford --
_tAptamer-drug conjugation for targeted tumor cell therapy /
_rMichael J. Donovan [and others] --
_tFive-step process for screening antisense compounds for efficacy: gene target IL-12Rb2 /
_rNikki B. Marshall, Laura L. Hauck, and Dan V. Mourich --
_tDiverse small non-coding RNAs in RNA interference pathways /
_rLiande Li and Yi Liu --
_tQuantification of siRNAs in vitro and in vivo /
_rAngie Cheng, Alexander V. Vlassov, and Susan Magdaleno --
_tOptimization of transfection conditions and analysis of siRNA potency using real-time PCR /
_rAngie Cheng, Susan Magdaleno, and Alexander V. Vlassov --
_tsiRNA knockdown of gene expression in endothelial cells /
_rEmily Dennstedt and Brad Bryan --
_tUsing RNA interference in Schistosoma mansoni /
_rRita Bhardwaj, Greice Krautz-Peterson, and Patrick J. Skelly --
_tPerforming the labeled microRNA pull-down (LAMP) assay system : an experimental approach for high-throughput identification of microRNA-target mRNAs /
_rRen-Jun Hsu and Huai-Jen Tsai --
_tSynthesis, purification, and characterization of oligoribonucleotides that act as agonists of TLR7 and/or TLR8 /
_rTao Lan and Ekambar R. Kandimalla --
_tSynthesis, purification, and characterization of immune-modulatory oligodeoxynucleotides that act as agonists of toll-like receptor 9 /
_rMallikarjuna Reddy Putta, Dong Yu, and Ekambar R. Kandimalla --
_tSurface plasmon resonance investigation of RNA aptamer-RNA ligand interactions /
_rCarmelo Di Primo, Eric Dausse, and Jean-Jacques Toulme --
_tPractical considerations for analyzing antigene RNAs (agRNAs) : RNA immunoprecipitation of argonaute protein /
_rJacob C. Schwartz and David R. Corey --
_tInhibition of human papillomavirus expression using DNAzymes /
_rMaria Luisa Benitez-Hess, Pablo Reyes-Gutierrez, and Luis Marat Alvarez-Salas.
520 _aThe field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.
546 _aEnglish.
650 0 _aOligonucleotides
_xTherapeutic use.
_987237
650 2 _aPolynucleotides.
_983858
650 2 _aNucleotides.
_983859
650 2 _aNucleic Acids, Nucleotides, and Nucleosides
_979966
650 2 _aChemicals and Drugs
_913222
650 2 _aOligonucleotides.
_983856
650 7 _aOligonucleotides
_xTherapeutic use.
_2fast
_0(OCoLC)fst01045458
_987237
653 0 0 _alevenswetenschappen
653 0 0 _alife sciences
653 0 0 _abiochemie
653 0 0 _abiochemistry
653 0 0 _anucleic acids
653 0 0 _achemie
653 0 0 _achemistry
653 1 0 _aBiology (General)
653 1 0 _aBiologie (algemeen)
655 4 _aElectronic books.
_9396
700 1 _aGoodchild, John,
_cchemist.
_987238
776 0 8 _iPrint version:
_tTherapeutic oligonucleotides.
_dNew York : Humana Press ; Springer, ©2011
_z1617791873
_w(DLC) 2011931964
_w(OCoLC)728580587
830 0 _aMethods in molecular biology (Clifton, N.J.) ;
_vv. 764.
_987239
830 0 _aSpringer protocols (Series)
_980328
856 4 0 _uhttps://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-61779-188-8
938 _aYBP Library Services
_bYANK
_n7476222
994 _a92
_bATIST
999 _c390809
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