000 07199cam a2200793Ia 4500
001 ocn828180138
003 OCoLC
005 20220204094831.0
006 m o d
007 cr cnu---unuuu
008 130222s2013 nyua ob 001 0 eng d
040 _aGW5XE
066 _c(S
016 7 _a016205914
019 _a964905115
020 _a9781627033176
_q(electronic bk.)
020 _a1627033173
_q(electronic bk.)
020 _z9781627033169
020 _z1627033165
024 7 _a10.1007/978-1-62703-317-6
029 1 _aAU@
029 1 _aNZ1
035 _a(OCoLC)828180138
050 4 _aQH588.S83
_bS74 2013
060 4 _aW1
_bME9616J v.978 2013
060 4 _aQU 375
072 7 _aPSF
072 7 _aSCI049000
082 0 4 _a616.02/774
049 _aMAIN
245 0 0 _aStem cells and aging :
_bmethods and protocols /
_cedited by Kursad Turksen.
260 _aNew York :
_bHumana Press :
300 _a1 online resource (xi, 179 pages) :
_billustrations (some color)
336 _atext
337 _acomputer
338 _aonline resource
347 _atext file
490 1 _aMethods in molecular biology,
_x1940-6029 ;
504 _aIncludes bibliographical references and index.
505 0 0 _tComprehensive hematopoietic stem cell isolation methods /
_rKyle Rector, Yi Liu, and Gary Van Zant --
_tSerial transplantation of bone marrow to test self-renewal capacity of hematopoietic stem cells in vivo /
_rCharusheila Ramkumar, Rachel M. Gerstein, and Hong Zhang --
_tMouse hematopoietic stem cell transplantation /
_rHui Cheng, Paulina H. Liang, and Tao Cheng --
_tIsolation, characterization, and transplantation of adult liver progenitor cells /
_rMladen I. Yovchev, Mariana D. Dabeva, and Michael Oertel --
_tIsolation of muscle-derived stem/progenitor cells based on adhesion characteristics to collagen-coated surfaces /
_rMitra Lavasani [and others] --
_tHuman myoblasts from skeletal muscle biopsies : in vitro culture preparations for morphological and cytochemical analyses at light and electron microscopy /
_rManuela Malatesta [and others] --
_tCardiac stem cell senescence /
_rDaniela Cesselli [and others] --
_tIsolation of mesenchymal stem cells from human bone and long-term cultivation under physiologic oxygen conditions /
_rSebastian Klepsch [and others] --
_tMethods for assessing effects of Wnt/b-catenin signaling in senescence of mesenchymal stem cells /
_rHai-jie Wang and Yu-zhen Tan --
_tIntra-femoral injection of human mesenchymal stem cells /
_rSindhu T. Mohanty and Ilaria Bellantuono --
_tTracking of replicative senescence in mesenchymal stem cells by colony-forming unit frequency /
_rAnne Schellenberg, Hatim Hemeda, and Wolfgang Wagner --
_tChromatin immunoprecipitation coupled by quantitative real-time PCR as a tool for analyzing epigenetic regulation of stem cell aging /
_rSeunghee Lee, Ji-Won Jung, and Kyung-Sun Kang --
_tQuantitative fluorescence in situ hybridization on paraffin embedded tissue /
_rMario Ricciardi, Mauro Krampera, and Marco Chilosi.
588 0 _aPrint version record.
520 _aOver time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome. Stem Cells and Aging: Methods and Protocols brings together chapters from expert contributors with protocols critical for exploring the biology of stem cell aging, all of which is key for understanding these age-related stem cell changes at a basic biology level and at the level of their impacts for regenerative medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy to use, Stem Cells and Aging: Methods and Protocols serves as an ideal reference to guide investigators toward further valuable answers to the problems of our aging population.
546 _aEnglish.
650 0 _aStem cells
_vLaboratory manuals.
650 0 _aAging
_xMolecular aspects
_vLaboratory manuals.
650 2 _aStem Cells.
650 4 _aBiology.
650 4 _aHealth & Biological Sciences.
650 4 _aCytology.
650 7 _aAging
_xMolecular aspects.
650 7 _aStem cells.
653 0 0 _acelbiologie
653 0 0 _acellular biology
653 0 0 _astamcellen
653 0 0 _astem cells
653 0 0 _ageneeskunde
653 0 0 _amedicine
653 1 0 _aMedicine (General)
653 1 0 _aGeneeskunde (algemeen)
655 4 _aElectronic books.
655 7 _aLaboratory manuals.
700 1 _aTurksen, Kursad.
776 0 8 _iPrint version:
_tStem cells and aging.
_dNew York : Humana Press ; Springer, ©2013
830 0 _aMethods in molecular biology (Clifton, N.J.) ;
_vv. 976.
856 4 0 _uhttps://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-62703-317-6
880 0 _6505-00/(S
_aComprehensive Hematopoietic Stem Cell Isolation Methods -- Serial Transplantation of Bone Marrow to Test Self-Renewal Capacity of Hematopoietic Stem Cells In Vivo -- Mouse Hematopoietic Stem Cell Transplantation -- Isolation, Characterization, and Transplantation of Adult Liver Progenitor Cells -- Isolation of Muscle-Derived Stem/Progenitor Cells Based on Adhesion Characteristics to Collagen-Coated Surfaces -- Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and Cytochemical Analyses at Light and Electron Microscopy -- Cardiac Stem Cell Senescence -- Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions -- Methods for Assessing Effects of Wnt/β-Catenin Signaling in Senescence of Mesenchymal Stem Cells -- Intra-Femoral Injection of Human Mesenchymal Stem Cells -- Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency -- Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging -- Quantitative Fluorescence In Situ Hybridization (Q-FISH) on Paraffine Embedded Tissue.
938 _aYBP Library Services
994 _a92
999 _c391059