000 05774cam a2200757Ii 4500
001 ocn830553847
003 OCoLC
003 OCoLC
005 20200626150524.0
006 m o d
007 cr |||||||||||
008 130320s2013 nyuac ob 001 0 eng d
040 _aSCB
_beng
_erda
_epn
_cSCB
_dSCB
_dGW5XE
_dCOO
_dOHS
_dMYG
_dOCLCQ
_dOCLCO
_dOCLCA
_dOCLCF
_dOCLCQ
_dYDXCP
_dUBY
_dEUW
_dVT2
_dLVT
_dOCLCO
_dOTZ
_dTFH
_dOCLCO
_dOCLCA
_dBUF
_dKIJ
_dOCLCO
_dOCLCQ
_dCASUM
_dMERER
_dOCLCQ
_dU3W
_dOCLCO
_dOCLCQ
_dOCLCO
_dOCLCQ
_dYOU
_dOCLCO
_dOCLCQ
_dOCLCO
_dOCLCQ
_dWURST
_dOCLCA
019 _a853260387
_a964891724
_a967623289
_a985051264
_a1005765115
_a1027514878
_a1081248833
020 _a9781627033459
_q(electronic bk.)
020 _a1627033459
_q(electronic bk.)
020 _z9781627033442
020 _z1627033440
024 7 _a10.1007/978-1-62703-345-9
_2doi
029 1 _aAU@
_b000061127226
029 1 _aNZ1
_b15178258
029 1 _aNZ1
_b16094300
035 _a(OCoLC)830553847
_z(OCoLC)853260387
_z(OCoLC)964891724
_z(OCoLC)967623289
_z(OCoLC)985051264
_z(OCoLC)1005765115
_z(OCoLC)1027514878
_z(OCoLC)1081248833
050 4 _aQP356
060 4 _aW1
_bME9616J v.995 2013
060 4 _aQU 25
072 7 _aPSAN
_2bicssc
072 7 _aMED057000
_2bisacsh
082 0 4 _a573.8
_223
049 _aMAIN
245 0 0 _aChemical neurobiology :
_bmethods and protocols /
_cedited by Matthew R. Banghart.
264 1 _aNew York :
_bHumana Press,
_c[2013]
264 2 _aNew York :
_bSpringer Science+Business Media
264 4 _c©2013
300 _a1 online resource (xii, 219 pages) :
_billustrations
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_2rda
490 1 _aMethods in molecular biology,
_x1940-6029 ;
_vv. 995
504 _aIncludes bibliographical references and index.
505 0 0 _tEngineering k(+) channels using semisynthesis --
_tChemical derivatization and purification of peptide-toxins for probing ion channel complexes --
_tUsing yeast to study potassium channel function and interactions with small molecules --
_tA FLIPR assay for evaluating agonists and antagonists of GPCR heterodimers --
_tCharacterizing caged molecules through flash photolysis and transient absorption spectroscopy --
_tCharacterization of one- and two-photon photochemical uncaging efficiency --
_tPhotochromic potassium channel blockers: Design and electrophysiological characterization --
_tA (1)h NMR assay for measuring the photostationary states of photoswitchable ligands --
_tDeveloping a photoreactive antagonist --
_tDevelopment and in vitro characterization of ratiometric and intensity-based fluorescent ion sensors --
_tCharacterization of voltage-sensitive dyes in living cells using two-photon excitation --
_tCharacterization and validation of fluorescent receptor ligands: A case study of the ionotropic serotonin receptor --
_tImaging single synaptic vesicles in mammalian central synapses with quantum dots --
_tDirected evolution of protein-based neurotransmitter sensors for MRI.
588 0 _aPrint version record.
520 _aMany advances in modern neuroscience are enabled by the availability of chemical tools that allow sensitive, precise, and quantitative measurements of, and control over, biological processes. These powerful reagents are widely used for investigating the nervous system at levels of detail ranging from ion channel structure to neural network dynamics. Recent advances in photochemistry, microscopy, and protein engineering have triggered a surge in the development and application of these interdisciplinary techniques. Chemical Neurobiology: Methods and Protocols assists with the design, characterization and validation of new chemical tools for neurobiology by providing detailed protocols of procedures and assays deemed essential for the successful development and implementation of such tools. Divided into three sections, topics cover chemical probes of membrane protein structure and function, photochemical control of protein and cellular function, and chemical probes for imaging in the nervous system. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Chemical Neurobiology: Methods and Protocols serves scientists at many levels, including students aiming to expand their perspective, laboratory researchers seeking technical guidance, and established investigators looking for creative solutions to their research problems in molecular, cellular and systems neuroscience.
650 0 _aNeurobiology.
_9837
650 0 _aNeurochemistry.
_92867
650 2 _aNeurobiology.
_9837
650 2 _aNeurochemistry.
_92867
650 7 _aNeurobiology.
_2fast
_0(OCoLC)fst01036315
_9837
650 7 _aNeurochemistry.
_2fast
_0(OCoLC)fst01036329
_92867
653 0 0 _abiomedische wetenschappen
653 0 0 _abiomedicine
653 0 0 _aneurowetenschap
653 0 0 _aneuroscience
653 0 0 _aneurobiologie
653 0 0 _aneurobiology
653 1 0 _aMedicine (General)
653 1 0 _aGeneeskunde (algemeen)
655 4 _aElectronic books.
_9396
700 1 _aBanghart, Matthew R.,
_eeditor.
_988662
776 0 8 _iPrint version:
_tChemical neurobiology.
_d[S.l.] : Humana, 2013
_z1627033440
_w(OCoLC)821217443
830 0 _aMethods in molecular biology (Clifton, N.J.) ;
_vv. 995.
_x1064-3745
_988663
856 4 0 _uhttps://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-62703-345-9
938 _aYBP Library Services
_bYANK
_n10436099
994 _a92
_bATIST
999 _c391066
_d391066