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008 130624s2013 nyua ob 001 0 eng d
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020 _a9781627034111
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020 _a1627034110
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020 _a1627034102
020 _a9781627034104
020 _z9781627034104
024 7 _a10.1007/978-1-62703-411-1
_2doi
029 1 _aAU@
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029 1 _aCHBIS
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035 _a(OCoLC)849902404
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050 4 _aQP625.N89
_bT75 2013
060 0 0 _aW1
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060 1 0 _aQU 25
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049 _aMAIN
245 0 0 _aTrinucleotide repeat protocols /
_cedited by Yoshinori Kohwi, Cynthia T. McMurray.
250 _a2nd ed.
260 _aNew York :
_bHumana Press,
_c©2013.
300 _a1 online resource (xiii, 296 pages) :
_billustrations.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in molecular biology,
_x1940-6029 ;
_vv. 1010
504 _aIncludes bibliographical references and index.
505 0 0 _tA brief history of triplet repeat diseases --
_tHuntington's disease and cell therapies: Past, present, and future --
_tTranscranial two-photon imaging of synaptic structures in the cortex of awake head-restrained mice --
_tIn vivo imaging of synapse plasticity in the mouse motor cortex --
_tPluripotent hybrid stem cells from transgenic Huntington's disease monkey --
_tMesenchymal stem cells for trinucleotide repeat disorders --
_tLentiviral-mediated gene transfer of siRNAs for the treatment of Huntington's disease --
_tPurification of dFMR1-containing complexes using tandem affinity purification --
_tCombined FISH and immunofluorescent staining methods to co-localize proteins and mRNA in neurons and brain tissue --
_tEpigenetic modifications of the FMR1 gene --
_tThe CGG repeat and the FMR1 gene --
_tStudy of oxidative damage and antioxidant systems in two Huntington's disease rodent models --
_tRegulation of ataxin-1 phosphorylation and its impact on biology --
_tTowards understanding region-specificity of triplet repeat diseases: Coupled immunohistology and mass spectrometry imaging --
_tAntibodies and intrabodies against huntingtin: Production and screening of monoclonals and single-chain recombinant forms --
_tCell recovery from DM1 transgenic mouse tissue to study (CTG) n instability and DM1 pathogenesis --
_tMarkerless modification of trinucleotide repeat loci in BACs --
_tDRPLA: Recent advances in research using transgenic mouse models.
520 _aTwo decades have passed since trinucleotide repeat expansion was first discovered in genes responsible for certain neurological diseases. Since then, new technologies have developed and innovative concepts have emerged, which may prove useful in devising therapeutic approaches to neurological diseases. Divided into six convenient sections, Trinucleotide Repeat Protocols, Second Edition covers a wide range of topics such as an overview of trinucleotide repeat diseases, synaptic plasticity, embryonic stem (ES) cell-related protocols with a focus on HD, RNA-related protocols, and analysis of epigenetic modification in fragile X syndrome. This edition focuses not only on direct analysis of trinucleotide repeat diseases but also on alternative approaches for the analysis of trinucleotide repeat diseases, with the hope that this will result in a better understanding of the mechanisms and future therapeutic prospects for treatment of these diseases. Written in the successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Trinucleotide Repeat Protocols, Second Edition seeks to serve researchers with its thorough methodologies on this expanding field.
546 _aEnglish.
650 0 _aTrinucleotide repeats
_vLaboratory manuals.
_981969
650 2 _aPublication Formats.
_915957
650 2 _aBiology.
_94937
650 2 _aMicrosatellite Repeats.
_988682
650 2 _aNeurodegenerative Diseases.
_980235
650 2 _aGenetic Diseases, Inborn.
_987701
650 2 _aTandem Repeat Sequences.
_988820
650 2 _aPublication Characteristics.
_915959
650 2 _aCongenital, Hereditary, and Neonatal Diseases and Abnormalities.
_988858
650 2 _aBiological Science Disciplines.
_912496
650 2 _aNervous System Diseases.
_980239
650 2 _aDisease.
_942313
650 2 _aGenome Components.
_986826
650 2 _aRepetitive Sequences, Nucleic Acid.
_988818
650 2 _aNatural Science Disciplines.
_912499
650 2 _aGenome.
_985892
650 2 _aBase Sequence.
_982573
650 2 _aDisciplines and Occupations.
_912501
650 2 _aGenetic Structures.
_980975
650 2 _aMolecular Structure.
_913251
650 2 _aBiochemical Phenomena.
_95597
650 2 _aGenetic Phenomena.
_94405
650 2 _aPhenomena and Processes.
_912502
650 2 _aChemical Phenomena.
_988859
650 2 _aLaboratory Manuals.
_981487
650 2 _aHeredodegenerative Disorders, Nervous System.
_988860
650 2 _aTrinucleotide Repeats.
_988861
650 2 _aGenetics.
_92539
650 7 _aTrinucleotide repeats.
_2fast
_0(OCoLC)fst01199837
_988862
650 7 _aHuman Anatomy & Physiology.
_2hilcc
_970648
650 7 _aHealth & Biological Sciences.
_2hilcc
_924083
650 7 _aAnimal Biochemistry.
_2hilcc
_970647
653 4 _aMedicine.
653 4 _aGene expression.
653 4 _aNeurology.
653 4 _aBiomedicine.
653 4 _aMolecular Medicine.
653 0 0 _ageneeskunde
653 0 0 _abiomedische wetenschappen
653 0 0 _agenexpressie
653 0 0 _aneurologie
653 1 0 _aMedicine (General)
653 1 0 _aGeneeskunde (algemeen)
655 4 _aElectronic books.
_9396
655 7 _aLaboratory manuals.
_2fast
_0(OCoLC)fst01920776
_988863
700 1 _aKohwi, Yoshinori.
_981974
700 1 _aMcMurray, Cynthia T.
_988864
776 0 8 _iPrint version:
_tTrinucleotide repeat protocols.
_b2nd ed.
_dNew York : Humana Press, ©2013
_w(DLC) 2013934713
830 0 _aMethods in molecular biology (Clifton, N.J.) ;
_vvolume 1010.
_x1064-3745
_988865
856 4 0 _uhttps://link-springer-com.libraryproxy.ist.ac.at/10.1007/978-1-62703-411-1
938 _aYBP Library Services
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